Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish
Zebrafish have an impressive capacity to regenerate neurons in the central nervous system. However, regeneration of the principal neuron of the evolutionary conserved cerebellum, the Purkinje cell (PC), is believed to be limited to developmental stages based on invasive lesions. In contrast, non-inv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/79672 |
| _version_ | 1797837629069197312 |
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| author | Sol Pose-Méndez Paul Schramm Barbara Winter Jochen C Meier Konstantinos Ampatzis Reinhard W Köster |
| author_facet | Sol Pose-Méndez Paul Schramm Barbara Winter Jochen C Meier Konstantinos Ampatzis Reinhard W Köster |
| author_sort | Sol Pose-Méndez |
| collection | DOAJ |
| description | Zebrafish have an impressive capacity to regenerate neurons in the central nervous system. However, regeneration of the principal neuron of the evolutionary conserved cerebellum, the Purkinje cell (PC), is believed to be limited to developmental stages based on invasive lesions. In contrast, non-invasive cell type-specific ablation by induced apoptosis closely represents a process of neurodegeneration. We demonstrate that the ablated larval PC population entirely recovers in number, quickly reestablishes electrophysiological properties, and properly integrates into circuits to regulate cerebellum-controlled behavior. PC progenitors are present in larvae and adults, and PC ablation in adult cerebelli results in an impressive PC regeneration of different PC subtypes able to restore behavioral impairments. Interestingly, caudal PCs are more resistant to ablation and regenerate more efficiently, suggesting a rostro-caudal pattern of de- and regeneration properties. These findings demonstrate that the zebrafish cerebellum is able to regenerate functional PCs during all stages of the animal’s life. |
| first_indexed | 2024-04-09T15:27:45Z |
| format | Article |
| id | doaj.art-40e18bb86e2b45ed8f30aba254501828 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T15:27:45Z |
| publishDate | 2023-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-40e18bb86e2b45ed8f30aba2545018282023-04-28T10:45:39ZengeLife Sciences Publications LtdeLife2050-084X2023-04-011210.7554/eLife.79672Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafishSol Pose-Méndez0Paul Schramm1https://orcid.org/0000-0002-0894-2348Barbara Winter2Jochen C Meier3Konstantinos Ampatzis4https://orcid.org/0000-0001-7998-6463Reinhard W Köster5https://orcid.org/0000-0001-6593-8196Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, GermanyCellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, GermanyCellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, GermanyCell Physiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, GermanyNeuroscience Department, Karolinska Institute, Stockholm, SwedenCellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, GermanyZebrafish have an impressive capacity to regenerate neurons in the central nervous system. However, regeneration of the principal neuron of the evolutionary conserved cerebellum, the Purkinje cell (PC), is believed to be limited to developmental stages based on invasive lesions. In contrast, non-invasive cell type-specific ablation by induced apoptosis closely represents a process of neurodegeneration. We demonstrate that the ablated larval PC population entirely recovers in number, quickly reestablishes electrophysiological properties, and properly integrates into circuits to regulate cerebellum-controlled behavior. PC progenitors are present in larvae and adults, and PC ablation in adult cerebelli results in an impressive PC regeneration of different PC subtypes able to restore behavioral impairments. Interestingly, caudal PCs are more resistant to ablation and regenerate more efficiently, suggesting a rostro-caudal pattern of de- and regeneration properties. These findings demonstrate that the zebrafish cerebellum is able to regenerate functional PCs during all stages of the animal’s life.https://elifesciences.org/articles/79672zebrafishcerebellumPurkinje cellscell ablationregeneration |
| spellingShingle | Sol Pose-Méndez Paul Schramm Barbara Winter Jochen C Meier Konstantinos Ampatzis Reinhard W Köster Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish eLife zebrafish cerebellum Purkinje cells cell ablation regeneration |
| title | Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish |
| title_full | Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish |
| title_fullStr | Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish |
| title_full_unstemmed | Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish |
| title_short | Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish |
| title_sort | lifelong regeneration of cerebellar purkinje cells after induced cell ablation in zebrafish |
| topic | zebrafish cerebellum Purkinje cells cell ablation regeneration |
| url | https://elifesciences.org/articles/79672 |
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