Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro
The effect of an extremely low frequency alternating magnetic field (ELF AMF) at frequencies of 17, 48, and 95 Hz at 100 mT on free and internalized 4T1 breast cancer cell submicron magnetic mineral carriers with an anticancer drug, mitoxantrone, was shown. The alternating magnetic field (100 mT; 17...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/2/443 |
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| author | Anatolii A. Abalymov Roman A. Anisimov Polina A. Demina Veronika A. Kildisheva Alexandra E. Kalinova Alexey A. Serdobintsev Nadezhda G. Novikova Dmitry B. Petrenko Alexandr V. Sadovnikov Denis V. Voronin Maria V. Lomova |
| author_facet | Anatolii A. Abalymov Roman A. Anisimov Polina A. Demina Veronika A. Kildisheva Alexandra E. Kalinova Alexey A. Serdobintsev Nadezhda G. Novikova Dmitry B. Petrenko Alexandr V. Sadovnikov Denis V. Voronin Maria V. Lomova |
| author_sort | Anatolii A. Abalymov |
| collection | DOAJ |
| description | The effect of an extremely low frequency alternating magnetic field (ELF AMF) at frequencies of 17, 48, and 95 Hz at 100 mT on free and internalized 4T1 breast cancer cell submicron magnetic mineral carriers with an anticancer drug, mitoxantrone, was shown. The alternating magnetic field (100 mT; 17, 48, 95 Hz; time of treatment—10.5 min with a 30 s delay) does not lead to the significant destruction of carrier shells and release of mitoxantrone or bovine serum albumin from them according to the data of spectrophotometry, or the heating of carriers in the process of exposure to magnetic fields. The most optimal set of factors that would lead to the suppression of proliferation and survival of cells with anticancer drug carriers on the third day (in comparison with the control and first day) is exposure to an alternating magnetic field of 100 mT in a pulsed mode with a frequency of 95 Hz. The presence of magnetic nanocarriers in cell lines was carried out by a direct label-free method, space-resolved Brillouin light scattering (BLS) spectrometry, which was realized for the first time. The analysis of the series of integrated BLS spectra showed an increase in the magnetic phase in cells with a growth in the number of particles per cell (from 10 to 100) after their internalization. The safety of magnetic carriers in the release of their constituent ions has been evaluated using atomic absorption spectrometry. |
| first_indexed | 2024-03-07T22:40:31Z |
| format | Article |
| id | doaj.art-40e349cbd9c24e5f91afd79d44ccb153 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-07T22:40:31Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-40e349cbd9c24e5f91afd79d44ccb1532024-02-23T15:08:51ZengMDPI AGBiomedicines2227-90592024-02-0112244310.3390/biomedicines12020443Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In VitroAnatolii A. Abalymov0Roman A. Anisimov1Polina A. Demina2Veronika A. Kildisheva3Alexandra E. Kalinova4Alexey A. Serdobintsev5Nadezhda G. Novikova6Dmitry B. Petrenko7Alexandr V. Sadovnikov8Denis V. Voronin9Maria V. Lomova10Science Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaScience Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaScience Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaScience Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaInstitute of Physics, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaInstitute of Physics, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaInstitute of Comprehensive Exploitation, Mineral Resources Russian Academy of Sciences, Moscow 111020, RussiaGeological Institute, Russian Academy of Sciences, Moscow 119017, RussiaInstitute of Physics, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaDepartment of Physical and Colloid Chemistry, National University of Oil and Gas “Gubkin University”, Moscow 119991, RussiaScience Medical Centre, Saratov State University, 83 Astrakhanskayast, Saratov 410012, RussiaThe effect of an extremely low frequency alternating magnetic field (ELF AMF) at frequencies of 17, 48, and 95 Hz at 100 mT on free and internalized 4T1 breast cancer cell submicron magnetic mineral carriers with an anticancer drug, mitoxantrone, was shown. The alternating magnetic field (100 mT; 17, 48, 95 Hz; time of treatment—10.5 min with a 30 s delay) does not lead to the significant destruction of carrier shells and release of mitoxantrone or bovine serum albumin from them according to the data of spectrophotometry, or the heating of carriers in the process of exposure to magnetic fields. The most optimal set of factors that would lead to the suppression of proliferation and survival of cells with anticancer drug carriers on the third day (in comparison with the control and first day) is exposure to an alternating magnetic field of 100 mT in a pulsed mode with a frequency of 95 Hz. The presence of magnetic nanocarriers in cell lines was carried out by a direct label-free method, space-resolved Brillouin light scattering (BLS) spectrometry, which was realized for the first time. The analysis of the series of integrated BLS spectra showed an increase in the magnetic phase in cells with a growth in the number of particles per cell (from 10 to 100) after their internalization. The safety of magnetic carriers in the release of their constituent ions has been evaluated using atomic absorption spectrometry.https://www.mdpi.com/2227-9059/12/2/443magnetic nanoparticlesmitoxantronealternative magnetic fieldcalcium carbonatebreast cancer |
| spellingShingle | Anatolii A. Abalymov Roman A. Anisimov Polina A. Demina Veronika A. Kildisheva Alexandra E. Kalinova Alexey A. Serdobintsev Nadezhda G. Novikova Dmitry B. Petrenko Alexandr V. Sadovnikov Denis V. Voronin Maria V. Lomova Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro Biomedicines magnetic nanoparticles mitoxantrone alternative magnetic field calcium carbonate breast cancer |
| title | Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro |
| title_full | Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro |
| title_fullStr | Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro |
| title_full_unstemmed | Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro |
| title_short | Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro |
| title_sort | time delayed anticancer effect of an extremely low frequency alternating magnetic field and multimodal protein tannin mitoxantrone carriers with brillouin microspectroscopy visualization in vitro |
| topic | magnetic nanoparticles mitoxantrone alternative magnetic field calcium carbonate breast cancer |
| url | https://www.mdpi.com/2227-9059/12/2/443 |
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