Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS
Background and Purpose Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify of Candida species isolated from vulvovaginitis woman suffering vulvovaginitis refered to Ghaem Hospital, Mashhad, Iran, by use of MALDI-TOF mass spectrometry. Mat...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2017-12-01
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| Series: | Current Medical Mycology |
| Subjects: | |
| Online Access: | http://cmm.mazums.ac.ir/browse.php?a_code=A-10-220-2&slc_lang=en&sid=1 |
| _version_ | 1828542875388870656 |
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| author | Majid Alizadeh Anna Kolecka Teun Boekhout Hossein Zarrinfar Mohamad Ali Ghanbari Nahzag Parisa Badiee Ali Rezaei-Matehkolaei Abdolmajid Fata Somayeh Dolatabadi Mohammad Javad Najafzadeh |
| author_facet | Majid Alizadeh Anna Kolecka Teun Boekhout Hossein Zarrinfar Mohamad Ali Ghanbari Nahzag Parisa Badiee Ali Rezaei-Matehkolaei Abdolmajid Fata Somayeh Dolatabadi Mohammad Javad Najafzadeh |
| author_sort | Majid Alizadeh |
| collection | DOAJ |
| description | Background and Purpose
Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify of Candida species isolated from vulvovaginitis woman suffering vulvovaginitis refered to Ghaem Hospital, Mashhad, Iran, by use of MALDI-TOF mass spectrometry.
Materials and Methods
The 65 clinical samples isolated from Vulvovaginitis women were collected in Ghaem Hospital. All specimens were identified using phenotypic techniques such as microscopy and culture on Sabouraud dextrose agar and corn meal agar medium,Then, All isolates were detected and were processed for MALDI TOF MS identification.
Results
Of the 65 isolates analyzed, 61 (93.8%) were recognised by MALDI-TOF mass spectrometry and for four isolates (6.1%) only not relabile identifications were achieved. In this study, the most frequently isolated species were Candida albicans (58.5%), followed by Candida tropicalis (16.9%), Candida glabrata (7.7%), Candida parapsilosis (7.7%) and Candida guillermondii (3.1%).
Conclusion
presented results demonstrate that the MALDI TOF mass spectrometry is a fast and reliable technique, and has the potential to replace conventional phenotypic identification of Candida species and other yeast strains routinely isolated in clinical microbiology laboratories. |
| first_indexed | 2024-12-12T02:03:52Z |
| format | Article |
| id | doaj.art-40e5377be7894810a5854ef362368c24 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-12-12T02:03:52Z |
| publishDate | 2017-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-40e5377be7894810a5854ef362368c242022-12-22T00:42:06ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202017-12-01342125Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MSMajid Alizadeh0Anna Kolecka1Teun Boekhout2Hossein Zarrinfar3Mohamad Ali Ghanbari Nahzag4Parisa Badiee5Ali Rezaei-Matehkolaei6Abdolmajid Fata7Somayeh Dolatabadi8Mohammad Javad Najafzadeh9 Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam, Amsterdam, The Netherlands Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Medical Mycology, School of Medicine, Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Department of Parasitology and Mycology, Research Center for Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Faculty of Engineering, Sabzevar University of New Technologies, Sabzevar, Iran Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Background and Purpose Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify of Candida species isolated from vulvovaginitis woman suffering vulvovaginitis refered to Ghaem Hospital, Mashhad, Iran, by use of MALDI-TOF mass spectrometry. Materials and Methods The 65 clinical samples isolated from Vulvovaginitis women were collected in Ghaem Hospital. All specimens were identified using phenotypic techniques such as microscopy and culture on Sabouraud dextrose agar and corn meal agar medium,Then, All isolates were detected and were processed for MALDI TOF MS identification. Results Of the 65 isolates analyzed, 61 (93.8%) were recognised by MALDI-TOF mass spectrometry and for four isolates (6.1%) only not relabile identifications were achieved. In this study, the most frequently isolated species were Candida albicans (58.5%), followed by Candida tropicalis (16.9%), Candida glabrata (7.7%), Candida parapsilosis (7.7%) and Candida guillermondii (3.1%). Conclusion presented results demonstrate that the MALDI TOF mass spectrometry is a fast and reliable technique, and has the potential to replace conventional phenotypic identification of Candida species and other yeast strains routinely isolated in clinical microbiology laboratories.http://cmm.mazums.ac.ir/browse.php?a_code=A-10-220-2&slc_lang=en&sid=1MALDI-TOF MS Candidiasis Vulvovaginitis identification |
| spellingShingle | Majid Alizadeh Anna Kolecka Teun Boekhout Hossein Zarrinfar Mohamad Ali Ghanbari Nahzag Parisa Badiee Ali Rezaei-Matehkolaei Abdolmajid Fata Somayeh Dolatabadi Mohammad Javad Najafzadeh Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS Current Medical Mycology MALDI-TOF MS Candidiasis Vulvovaginitis identification |
| title | Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS |
| title_full | Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS |
| title_fullStr | Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS |
| title_full_unstemmed | Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS |
| title_short | Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS |
| title_sort | identification of candida species isolated from vulvovaginitis in mashhad iran by use of maldi tof ms |
| topic | MALDI-TOF MS Candidiasis Vulvovaginitis identification |
| url | http://cmm.mazums.ac.ir/browse.php?a_code=A-10-220-2&slc_lang=en&sid=1 |
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