Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss
Objectives Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant func...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2023-05-01
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| Series: | Clinical and Experimental Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.e-ceo.org/upload/pdf/ceo-2022-01235.pdf |
| _version_ | 1797821487221047296 |
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| author | Janghyun Kim Bora Lee Sungsu Lee Joon-Tae Kim Byeong C. Kim Hyong-Ho Cho |
| author_facet | Janghyun Kim Bora Lee Sungsu Lee Joon-Tae Kim Byeong C. Kim Hyong-Ho Cho |
| author_sort | Janghyun Kim |
| collection | DOAJ |
| description | Objectives Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant function are associated with subsequent cochlear senescence in response to aging stress. Long non-coding RNA (lncRNA) has been reported to play important roles in various diseases. However, the function of lncRNA in ARHL remains unclear. In this study, we analyzed the common expression profiles of messenger RNA (mRNA) and lncRNA through ARHL-related RNA-sequencing datasets. Methods We selected and downloaded three different sets of RNA-sequencing data for ARHL. We performed differential expression analysis to find common mRNA and lncRNA profiles in the cochleae of aged mice compared to young mice. Gene Ontology (GO) analysis was used for functional exploration. Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate mRNAs and lncRNAs. In addition, we performed trans target prediction analysis with differentially expressed mRNAs and lncRNAs to understand the function of these mRNAs and lncRNAs in ARHL. Results We identified 112 common mRNAs and 10 common lncRNAs in the cochleae of aged mice compared to young mice. GO analysis showed that the 112 upregulated mRNAs were enriched in the defense response pathway. When we performed qRT-PCR with 1 mM H2O2-treated House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, the qRT-PCR results were consistent with the RNA-sequencing analysis data. lncRNA-mRNA networks were constructed using the 10 common lncRNAs and 112 common mRNAs in ARHL. Conclusion Our study provides a comprehensive understanding of the common mRNA and lncRNA expression profiles in ARHL. Knowledge of ARHL-associated mRNAs and lncRNAs could be useful for better understanding ARHL and these mRNAs and lncRNAs might be a potential therapeutic target for preventing ARHL. |
| first_indexed | 2024-03-13T09:53:23Z |
| format | Article |
| id | doaj.art-40e5fab862cc4c16b2f01634096adbbc |
| institution | Directory Open Access Journal |
| issn | 1976-8710 2005-0720 |
| language | English |
| last_indexed | 2024-03-13T09:53:23Z |
| publishDate | 2023-05-01 |
| publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
| record_format | Article |
| series | Clinical and Experimental Otorhinolaryngology |
| spelling | doaj.art-40e5fab862cc4c16b2f01634096adbbc2023-05-24T05:26:20ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202023-05-0116211512410.21053/ceo.2022.01235853Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing LossJanghyun Kim0Bora Lee1Sungsu Lee2Joon-Tae Kim3Byeong C. Kim4Hyong-Ho Cho5 Department of Neurology, Chonnam National University Hospital, Gwangju, Korea Department of Biochemistry, Chonnam National University Medical School, Gwangju, Korea Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea Department of Neurology, Chonnam National University Hospital, Gwangju, Korea Department of Neurology, Chonnam National University Hospital, Gwangju, Korea Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, KoreaObjectives Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant function are associated with subsequent cochlear senescence in response to aging stress. Long non-coding RNA (lncRNA) has been reported to play important roles in various diseases. However, the function of lncRNA in ARHL remains unclear. In this study, we analyzed the common expression profiles of messenger RNA (mRNA) and lncRNA through ARHL-related RNA-sequencing datasets. Methods We selected and downloaded three different sets of RNA-sequencing data for ARHL. We performed differential expression analysis to find common mRNA and lncRNA profiles in the cochleae of aged mice compared to young mice. Gene Ontology (GO) analysis was used for functional exploration. Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate mRNAs and lncRNAs. In addition, we performed trans target prediction analysis with differentially expressed mRNAs and lncRNAs to understand the function of these mRNAs and lncRNAs in ARHL. Results We identified 112 common mRNAs and 10 common lncRNAs in the cochleae of aged mice compared to young mice. GO analysis showed that the 112 upregulated mRNAs were enriched in the defense response pathway. When we performed qRT-PCR with 1 mM H2O2-treated House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, the qRT-PCR results were consistent with the RNA-sequencing analysis data. lncRNA-mRNA networks were constructed using the 10 common lncRNAs and 112 common mRNAs in ARHL. Conclusion Our study provides a comprehensive understanding of the common mRNA and lncRNA expression profiles in ARHL. Knowledge of ARHL-associated mRNAs and lncRNAs could be useful for better understanding ARHL and these mRNAs and lncRNAs might be a potential therapeutic target for preventing ARHL.http://www.e-ceo.org/upload/pdf/ceo-2022-01235.pdfpresbycusishearing losslong noncoding rnamessenger rna |
| spellingShingle | Janghyun Kim Bora Lee Sungsu Lee Joon-Tae Kim Byeong C. Kim Hyong-Ho Cho Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss Clinical and Experimental Otorhinolaryngology presbycusis hearing loss long noncoding rna messenger rna |
| title | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
| title_full | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
| title_fullStr | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
| title_full_unstemmed | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
| title_short | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
| title_sort | identification and characterization of mrna and lncrna expression profiles in age related hearing loss |
| topic | presbycusis hearing loss long noncoding rna messenger rna |
| url | http://www.e-ceo.org/upload/pdf/ceo-2022-01235.pdf |
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