Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure.
Marburg virus infection in humans causes a hemorrhagic disease with a high case fatality rate. Countermeasure development requires the use of well-characterized animal models that mimic human disease. To further characterize the cynomolgus macaque model of MARV/Angola, two independent dose response...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4586374?pdf=render |
| _version_ | 1818236990372446208 |
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| author | Sara C Johnston Kenny L Lin Nancy A Twenhafel Jo Lynne W Raymond Joshua D Shamblin Suzanne E Wollen Carly B Wlazlowski Eric R Wilkinson Miriam A Botto Arthur J Goff |
| author_facet | Sara C Johnston Kenny L Lin Nancy A Twenhafel Jo Lynne W Raymond Joshua D Shamblin Suzanne E Wollen Carly B Wlazlowski Eric R Wilkinson Miriam A Botto Arthur J Goff |
| author_sort | Sara C Johnston |
| collection | DOAJ |
| description | Marburg virus infection in humans causes a hemorrhagic disease with a high case fatality rate. Countermeasure development requires the use of well-characterized animal models that mimic human disease. To further characterize the cynomolgus macaque model of MARV/Angola, two independent dose response studies were performed using the intramuscular or aerosol routes of exposure. All animals succumbed at the lowest target dose; therefore, a dose effect could not be determined. For intramuscular-exposed animals, 100 PFU was the first target dose that was not significantly different than higher target doses in terms of time to disposition, clinical pathology, and histopathology. Although a significant difference was not observed between aerosol-exposed animals in the 10 PFU and 100 PFU target dose groups, 100 PFU was determined to be the lowest target dose that could be consistently obtained and accurately titrated in aerosol studies. |
| first_indexed | 2024-12-12T12:18:38Z |
| format | Article |
| id | doaj.art-40ea2eb5b327405fba8c961b00b1b7b6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T12:18:38Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-40ea2eb5b327405fba8c961b00b1b7b62022-12-22T00:24:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013884310.1371/journal.pone.0138843Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure.Sara C JohnstonKenny L LinNancy A TwenhafelJo Lynne W RaymondJoshua D ShamblinSuzanne E WollenCarly B WlazlowskiEric R WilkinsonMiriam A BottoArthur J GoffMarburg virus infection in humans causes a hemorrhagic disease with a high case fatality rate. Countermeasure development requires the use of well-characterized animal models that mimic human disease. To further characterize the cynomolgus macaque model of MARV/Angola, two independent dose response studies were performed using the intramuscular or aerosol routes of exposure. All animals succumbed at the lowest target dose; therefore, a dose effect could not be determined. For intramuscular-exposed animals, 100 PFU was the first target dose that was not significantly different than higher target doses in terms of time to disposition, clinical pathology, and histopathology. Although a significant difference was not observed between aerosol-exposed animals in the 10 PFU and 100 PFU target dose groups, 100 PFU was determined to be the lowest target dose that could be consistently obtained and accurately titrated in aerosol studies.http://europepmc.org/articles/PMC4586374?pdf=render |
| spellingShingle | Sara C Johnston Kenny L Lin Nancy A Twenhafel Jo Lynne W Raymond Joshua D Shamblin Suzanne E Wollen Carly B Wlazlowski Eric R Wilkinson Miriam A Botto Arthur J Goff Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. PLoS ONE |
| title | Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. |
| title_full | Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. |
| title_fullStr | Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. |
| title_full_unstemmed | Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. |
| title_short | Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure. |
| title_sort | dose response of marv angola infection in cynomolgus macaques following im or aerosol exposure |
| url | http://europepmc.org/articles/PMC4586374?pdf=render |
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