Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd
The aim of work was to study the influence of flurenizyd (antimicrobial, antituberculosis, antichlamydia, immunomodulatory, antioxidant, hepatoprotective, anti-inflammatory medicine) on the antioxidant homoeostasis of loach embryos (Misgurnus fossilis L.) during early embryogenesis. Glutathioneperox...
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| Format: | Article |
| Language: | English |
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V.N. Karazin Kharkiv National University
2016-07-01
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| Series: | Біофізичний вісник |
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| Online Access: | http://periodicals.karazin.ua/biophysvisnyk/article/view/6110 |
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| author | Н. О. Боднарчук Н. П. Гарасим Л. І. Петрух Д. І. Санагурський |
| author_facet | Н. О. Боднарчук Н. П. Гарасим Л. І. Петрух Д. І. Санагурський |
| author_sort | Н. О. Боднарчук |
| collection | DOAJ |
| description | The aim of work was to study the influence of flurenizyd (antimicrobial, antituberculosis, antichlamydia, immunomodulatory, antioxidant, hepatoprotective, anti-inflammatory medicine) on the antioxidant homoeostasis of loach embryos (Misgurnus fossilis L.) during early embryogenesis. Glutathioneperoxidase and glutathione-S-transferase activity was studied under the action of flurenizyd in concentrations 0.01; 0.05; 0.15; 1; 5; 15 mM, at the embryos of loach on the stage of development of the first (2 blastodmeres), fourth (16 blastodmeres), sixth (64 blastodmeres), eighth (256 blastodmeres), tenth (1024 blastodmeres) division of zygote (before the stage of de-synchronization). The two-factor analysis of variance was conducted to figure out the degree of flurenizyd influence, time of development and miscellaneous factors on activity of the enzymes of cell’s glutathione antioxidant defence system. It was shown that flurenizyd violates work of glutathioneperoxidase on all stages of development of loach embryos in particular predetermines the increase of their activity on the stage of development of 2, 16 and 256 blastomeres. The investigated antibiotic violates work of glutathione-S-transferase in the process of early embryogenesis of loach embryos Misgurnus fossilis L. In a maximal concentration (15 мМ) flurenizyd decreases activity of enzyme, starting from the initial stages of development of loach embryos. Two-factor analysis of variance indicated, that on glutathioneperoxidase and glutathione-S-transferase activity of loach embryos considerable influence is made by the miscellaneous factors, like external factors affecting the development of embryos. Time of development, in a greater degree, influences the work of glutathioneperoxidase during early embryogenesis, compared to flurenizyd that indicates indirect influence of the investigated antibiotic on glutathioneperoxidase. Considerable influence of flurenizyd on glutathione-S-transferase activity testifies its direct effect on a structure of the enzyme. |
| first_indexed | 2024-12-12T14:14:03Z |
| format | Article |
| id | doaj.art-40ecb0ad54d84b00a85ba0827ba358b6 |
| institution | Directory Open Access Journal |
| issn | 2075-3810 2075-3829 |
| language | English |
| last_indexed | 2024-12-12T14:14:03Z |
| publishDate | 2016-07-01 |
| publisher | V.N. Karazin Kharkiv National University |
| record_format | Article |
| series | Біофізичний вісник |
| spelling | doaj.art-40ecb0ad54d84b00a85ba0827ba358b62022-12-22T00:21:58ZengV.N. Karazin Kharkiv National UniversityБіофізичний вісник2075-38102075-38292016-07-0123443516032Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizydН. О. БоднарчукН. П. ГарасимЛ. І. ПетрухД. І. СанагурськийThe aim of work was to study the influence of flurenizyd (antimicrobial, antituberculosis, antichlamydia, immunomodulatory, antioxidant, hepatoprotective, anti-inflammatory medicine) on the antioxidant homoeostasis of loach embryos (Misgurnus fossilis L.) during early embryogenesis. Glutathioneperoxidase and glutathione-S-transferase activity was studied under the action of flurenizyd in concentrations 0.01; 0.05; 0.15; 1; 5; 15 mM, at the embryos of loach on the stage of development of the first (2 blastodmeres), fourth (16 blastodmeres), sixth (64 blastodmeres), eighth (256 blastodmeres), tenth (1024 blastodmeres) division of zygote (before the stage of de-synchronization). The two-factor analysis of variance was conducted to figure out the degree of flurenizyd influence, time of development and miscellaneous factors on activity of the enzymes of cell’s glutathione antioxidant defence system. It was shown that flurenizyd violates work of glutathioneperoxidase on all stages of development of loach embryos in particular predetermines the increase of their activity on the stage of development of 2, 16 and 256 blastomeres. The investigated antibiotic violates work of glutathione-S-transferase in the process of early embryogenesis of loach embryos Misgurnus fossilis L. In a maximal concentration (15 мМ) flurenizyd decreases activity of enzyme, starting from the initial stages of development of loach embryos. Two-factor analysis of variance indicated, that on glutathioneperoxidase and glutathione-S-transferase activity of loach embryos considerable influence is made by the miscellaneous factors, like external factors affecting the development of embryos. Time of development, in a greater degree, influences the work of glutathioneperoxidase during early embryogenesis, compared to flurenizyd that indicates indirect influence of the investigated antibiotic on glutathioneperoxidase. Considerable influence of flurenizyd on glutathione-S-transferase activity testifies its direct effect on a structure of the enzyme.http://periodicals.karazin.ua/biophysvisnyk/article/view/6110зародки в’юнафлуренізидембріогенезглутатіонпероксидазаглутатіон-S-трансфераза |
| spellingShingle | Н. О. Боднарчук Н. П. Гарасим Л. І. Петрух Д. І. Санагурський Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd Біофізичний вісник зародки в’юна флуренізид ембріогенез глутатіонпероксидаза глутатіон-S-трансфераза |
| title | Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd |
| title_full | Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd |
| title_fullStr | Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd |
| title_full_unstemmed | Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd |
| title_short | Comparative and variance analysis of activity of glutation dependent enzymes in loach Misgurnus fossilis L. embryos under the influence of flurenizyd |
| title_sort | comparative and variance analysis of activity of glutation dependent enzymes in loach misgurnus fossilis l embryos under the influence of flurenizyd |
| topic | зародки в’юна флуренізид ембріогенез глутатіонпероксидаза глутатіон-S-трансфераза |
| url | http://periodicals.karazin.ua/biophysvisnyk/article/view/6110 |
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