Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids.
Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid was tested as internal standard for high-pressure liquid chromatographic analysis of the five main glycine- and taurine-conjugated bile acids present in adult human serum and bile. When the standard is added to the samples before extraction, the...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1985-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520343066 |
| _version_ | 1818688753745526784 |
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| author | M Muraca Y Ghoos |
| author_facet | M Muraca Y Ghoos |
| author_sort | M Muraca |
| collection | DOAJ |
| description | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid was tested as internal standard for high-pressure liquid chromatographic analysis of the five main glycine- and taurine-conjugated bile acids present in adult human serum and bile. When the standard is added to the samples before extraction, the recovery rate throughout the procedure is similar to that of other bile acids. For all bile acids studied, the response, relative to the internal standard, is linear at 205 nm. Baseline separation is observed between the internal standard and all other bile acids, both in artificial mixtures and extracts of biological samples. Thus, glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid is a reliable internal standard for HPLC analysis of conjugated bile acids in serum and bile. |
| first_indexed | 2024-12-17T11:59:13Z |
| format | Article |
| id | doaj.art-40ed902d0d2b4efd8f56d3b74d8bb9c5 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T11:59:13Z |
| publishDate | 1985-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-40ed902d0d2b4efd8f56d3b74d8bb9c52022-12-21T21:49:54ZengElsevierJournal of Lipid Research0022-22751985-08-0126810091011Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids.M MuracaY GhoosGlyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid was tested as internal standard for high-pressure liquid chromatographic analysis of the five main glycine- and taurine-conjugated bile acids present in adult human serum and bile. When the standard is added to the samples before extraction, the recovery rate throughout the procedure is similar to that of other bile acids. For all bile acids studied, the response, relative to the internal standard, is linear at 205 nm. Baseline separation is observed between the internal standard and all other bile acids, both in artificial mixtures and extracts of biological samples. Thus, glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid is a reliable internal standard for HPLC analysis of conjugated bile acids in serum and bile.http://www.sciencedirect.com/science/article/pii/S0022227520343066 |
| spellingShingle | M Muraca Y Ghoos Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. Journal of Lipid Research |
| title | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. |
| title_full | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. |
| title_fullStr | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. |
| title_full_unstemmed | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. |
| title_short | Glyco-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard for high-pressure liquid chromatographic analysis of conjugated bile acids. |
| title_sort | glyco 7 alpha 12 alpha dihydroxy 5 beta cholanic acid as internal standard for high pressure liquid chromatographic analysis of conjugated bile acids |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520343066 |
| work_keys_str_mv | AT mmuraca glyco7alpha12alphadihydroxy5betacholanicacidasinternalstandardforhighpressureliquidchromatographicanalysisofconjugatedbileacids AT yghoos glyco7alpha12alphadihydroxy5betacholanicacidasinternalstandardforhighpressureliquidchromatographicanalysisofconjugatedbileacids |