Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
ObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)th...
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Frontiers Media S.A.
2022-11-01
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Series: | Frontiers in Endocrinology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/full |
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author | Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Mingming Shu Mingming Shu Yiming Guo Shun Yao Shun Yao Shun Yao Yiran Wang Yiran Wang Yiran Wang Shujie Han Chunlan Song Chunlan Song Yunhai Chuai Yunhai Chuai Qihang Wang Fu Ma Fu Chen Ming Zhou Ming Zhou Wei Shang Wei Shang Wei Shang Wei Shang Wei Shang |
author_facet | Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Mingming Shu Mingming Shu Yiming Guo Shun Yao Shun Yao Shun Yao Yiran Wang Yiran Wang Yiran Wang Shujie Han Chunlan Song Chunlan Song Yunhai Chuai Yunhai Chuai Qihang Wang Fu Ma Fu Chen Ming Zhou Ming Zhou Wei Shang Wei Shang Wei Shang Wei Shang Wei Shang |
author_sort | Xinyu Zhai |
collection | DOAJ |
description | ObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)the luteal phase support.Main outcome measure(s)According to whether human chorionic gonadotropin(HCG) was added, the two groups were divided into an observation group and a control group, and the clinical outcomes of the two groups were compared. Furthermore, 20 patients were selected for whole exome sequencing to investigate the mechanism.ResultsThe observation group’s clinical pregnancy rate and live birth rate were significantly higher than those in the control group (P=0.004). Functional enrichment analysis showed that these genes were significantly enriched in embryo implantation or endometrial receptivity processes, such as microtubule-based movement, NABA CORE MATRISOME, superoxide anion generation, protein localization to vacuole, extracellular matrix organization, fertilization, microtubule-based transport, cell junction organization, microtubule cytoskeleton organization. Furthermore, variants detected in these pathway genes were missense mutations that affect the protein’s biological activity but do not effectuate its inactivation.ConclusionsAdding HCG in the luteal phase might improve the clinical pregnancy and live birth rates in RIF patients. The potential pathogenesis of RIF genetic level may be caused by microtubule-based movement, extracellular matrix organization, and the Superoxide Anion generation pathway. |
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issn | 1664-2392 |
language | English |
last_indexed | 2024-04-11T13:51:22Z |
publishDate | 2022-11-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Endocrinology |
spelling | doaj.art-40ef4cba7c0447439b66e3ccf5382f1b2022-12-22T04:20:34ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-11-011310.3389/fendo.2022.10535921053592Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failureXinyu Zhai0Xinyu Zhai1Xinyu Zhai2Xinyu Zhai3Xinyu Zhai4Mingming Shu5Mingming Shu6Yiming Guo7Shun Yao8Shun Yao9Shun Yao10Yiran Wang11Yiran Wang12Yiran Wang13Shujie Han14Chunlan Song15Chunlan Song16Yunhai Chuai17Yunhai Chuai18Qihang Wang19Fu Ma20Fu Chen21Ming Zhou22Ming Zhou23Wei Shang24Wei Shang25Wei Shang26Wei Shang27Wei Shang28Navy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Biology, Kenneth P. Dietrich School of Art and Science, University of Pittsburgh, Pittsburgh, PA, United StatesNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, First Hospital of Tsinghua University, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)the luteal phase support.Main outcome measure(s)According to whether human chorionic gonadotropin(HCG) was added, the two groups were divided into an observation group and a control group, and the clinical outcomes of the two groups were compared. Furthermore, 20 patients were selected for whole exome sequencing to investigate the mechanism.ResultsThe observation group’s clinical pregnancy rate and live birth rate were significantly higher than those in the control group (P=0.004). Functional enrichment analysis showed that these genes were significantly enriched in embryo implantation or endometrial receptivity processes, such as microtubule-based movement, NABA CORE MATRISOME, superoxide anion generation, protein localization to vacuole, extracellular matrix organization, fertilization, microtubule-based transport, cell junction organization, microtubule cytoskeleton organization. Furthermore, variants detected in these pathway genes were missense mutations that affect the protein’s biological activity but do not effectuate its inactivation.ConclusionsAdding HCG in the luteal phase might improve the clinical pregnancy and live birth rates in RIF patients. The potential pathogenesis of RIF genetic level may be caused by microtubule-based movement, extracellular matrix organization, and the Superoxide Anion generation pathway.https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/fullhuman chorionic gonadotropinrecurrent implantation failure (RIF)whole exome sequencing (WES)functional enrichment analysisgene mutation |
spellingShingle | Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Xinyu Zhai Mingming Shu Mingming Shu Yiming Guo Shun Yao Shun Yao Shun Yao Yiran Wang Yiran Wang Yiran Wang Shujie Han Chunlan Song Chunlan Song Yunhai Chuai Yunhai Chuai Qihang Wang Fu Ma Fu Chen Ming Zhou Ming Zhou Wei Shang Wei Shang Wei Shang Wei Shang Wei Shang Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure Frontiers in Endocrinology human chorionic gonadotropin recurrent implantation failure (RIF) whole exome sequencing (WES) functional enrichment analysis gene mutation |
title | Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure |
title_full | Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure |
title_fullStr | Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure |
title_full_unstemmed | Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure |
title_short | Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure |
title_sort | efficacy of low dose hcg on fet cycle in patients with recurrent implantation failure |
topic | human chorionic gonadotropin recurrent implantation failure (RIF) whole exome sequencing (WES) functional enrichment analysis gene mutation |
url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/full |
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