Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure

ObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)th...

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Main Authors: Xinyu Zhai, Mingming Shu, Yiming Guo, Shun Yao, Yiran Wang, Shujie Han, Chunlan Song, Yunhai Chuai, Qihang Wang, Fu Ma, Fu Chen, Ming Zhou, Wei Shang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Endocrinology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/full
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author Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Mingming Shu
Mingming Shu
Yiming Guo
Shun Yao
Shun Yao
Shun Yao
Yiran Wang
Yiran Wang
Yiran Wang
Shujie Han
Chunlan Song
Chunlan Song
Yunhai Chuai
Yunhai Chuai
Qihang Wang
Fu Ma
Fu Chen
Ming Zhou
Ming Zhou
Wei Shang
Wei Shang
Wei Shang
Wei Shang
Wei Shang
author_facet Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Mingming Shu
Mingming Shu
Yiming Guo
Shun Yao
Shun Yao
Shun Yao
Yiran Wang
Yiran Wang
Yiran Wang
Shujie Han
Chunlan Song
Chunlan Song
Yunhai Chuai
Yunhai Chuai
Qihang Wang
Fu Ma
Fu Chen
Ming Zhou
Ming Zhou
Wei Shang
Wei Shang
Wei Shang
Wei Shang
Wei Shang
author_sort Xinyu Zhai
collection DOAJ
description ObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)the luteal phase support.Main outcome measure(s)According to whether human chorionic gonadotropin(HCG) was added, the two groups were divided into an observation group and a control group, and the clinical outcomes of the two groups were compared. Furthermore, 20 patients were selected for whole exome sequencing to investigate the mechanism.ResultsThe observation group’s clinical pregnancy rate and live birth rate were significantly higher than those in the control group (P=0.004). Functional enrichment analysis showed that these genes were significantly enriched in embryo implantation or endometrial receptivity processes, such as microtubule-based movement, NABA CORE MATRISOME, superoxide anion generation, protein localization to vacuole, extracellular matrix organization, fertilization, microtubule-based transport, cell junction organization, microtubule cytoskeleton organization. Furthermore, variants detected in these pathway genes were missense mutations that affect the protein’s biological activity but do not effectuate its inactivation.ConclusionsAdding HCG in the luteal phase might improve the clinical pregnancy and live birth rates in RIF patients. The potential pathogenesis of RIF genetic level may be caused by microtubule-based movement, extracellular matrix organization, and the Superoxide Anion generation pathway.
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spelling doaj.art-40ef4cba7c0447439b66e3ccf5382f1b2022-12-22T04:20:34ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-11-011310.3389/fendo.2022.10535921053592Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failureXinyu Zhai0Xinyu Zhai1Xinyu Zhai2Xinyu Zhai3Xinyu Zhai4Mingming Shu5Mingming Shu6Yiming Guo7Shun Yao8Shun Yao9Shun Yao10Yiran Wang11Yiran Wang12Yiran Wang13Shujie Han14Chunlan Song15Chunlan Song16Yunhai Chuai17Yunhai Chuai18Qihang Wang19Fu Ma20Fu Chen21Ming Zhou22Ming Zhou23Wei Shang24Wei Shang25Wei Shang26Wei Shang27Wei Shang28Navy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Biology, Kenneth P. Dietrich School of Art and Science, University of Pittsburgh, Pittsburgh, PA, United StatesNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, First Hospital of Tsinghua University, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaNavy Clinical Medical School, Anhui Medical University, Hefei, ChinaThe Fifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaObjectiveTo study patients’ new treatment methods and mechanisms of repeated implantation failure.DesignA retrospective study.SettingIn vitro fertilization (IVF) unit in a Three-A hospital.Patient(s)Ninety-three patients with repeated implantation failure in IVF and embryo transfer.Intervention(s)the luteal phase support.Main outcome measure(s)According to whether human chorionic gonadotropin(HCG) was added, the two groups were divided into an observation group and a control group, and the clinical outcomes of the two groups were compared. Furthermore, 20 patients were selected for whole exome sequencing to investigate the mechanism.ResultsThe observation group’s clinical pregnancy rate and live birth rate were significantly higher than those in the control group (P=0.004). Functional enrichment analysis showed that these genes were significantly enriched in embryo implantation or endometrial receptivity processes, such as microtubule-based movement, NABA CORE MATRISOME, superoxide anion generation, protein localization to vacuole, extracellular matrix organization, fertilization, microtubule-based transport, cell junction organization, microtubule cytoskeleton organization. Furthermore, variants detected in these pathway genes were missense mutations that affect the protein’s biological activity but do not effectuate its inactivation.ConclusionsAdding HCG in the luteal phase might improve the clinical pregnancy and live birth rates in RIF patients. The potential pathogenesis of RIF genetic level may be caused by microtubule-based movement, extracellular matrix organization, and the Superoxide Anion generation pathway.https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/fullhuman chorionic gonadotropinrecurrent implantation failure (RIF)whole exome sequencing (WES)functional enrichment analysisgene mutation
spellingShingle Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Xinyu Zhai
Mingming Shu
Mingming Shu
Yiming Guo
Shun Yao
Shun Yao
Shun Yao
Yiran Wang
Yiran Wang
Yiran Wang
Shujie Han
Chunlan Song
Chunlan Song
Yunhai Chuai
Yunhai Chuai
Qihang Wang
Fu Ma
Fu Chen
Ming Zhou
Ming Zhou
Wei Shang
Wei Shang
Wei Shang
Wei Shang
Wei Shang
Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
Frontiers in Endocrinology
human chorionic gonadotropin
recurrent implantation failure (RIF)
whole exome sequencing (WES)
functional enrichment analysis
gene mutation
title Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
title_full Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
title_fullStr Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
title_full_unstemmed Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
title_short Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure
title_sort efficacy of low dose hcg on fet cycle in patients with recurrent implantation failure
topic human chorionic gonadotropin
recurrent implantation failure (RIF)
whole exome sequencing (WES)
functional enrichment analysis
gene mutation
url https://www.frontiersin.org/articles/10.3389/fendo.2022.1053592/full
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