Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids
Hybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F<sub>1</sub> hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus <i>Nicotiana</i>, <i>N. debn...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/10/2062 |
| _version_ | 1797513334890692608 |
|---|---|
| author | Takahiro Tezuka Naoto Kitamura Sae Imagawa Akira Hasegawa Kumpei Shiragaki Hai He Masanori Yanase Yoshiyuki Ogata Toshinobu Morikawa Shuji Yokoi |
| author_facet | Takahiro Tezuka Naoto Kitamura Sae Imagawa Akira Hasegawa Kumpei Shiragaki Hai He Masanori Yanase Yoshiyuki Ogata Toshinobu Morikawa Shuji Yokoi |
| author_sort | Takahiro Tezuka |
| collection | DOAJ |
| description | Hybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F<sub>1</sub> hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus <i>Nicotiana</i>, <i>N. debneyi</i> has the dominant allele <i>Hla1-1</i> at the <i>HLA1</i> locus that causes hybrid lethality in F<sub>1</sub> hybrid seedlings by interaction with <i>N. tabacum</i> allele(s). Here, we mapped the <i>HLA1</i> locus using the F<sub>2</sub> population segregating for the <i>Hla1-1</i> allele derived from the interspecific cross between <i>N. debneyi</i> and <i>N. fragrans</i>. To map <i>HLA1</i>, several DNA markers including random amplified polymorphic DNA, amplified fragment length polymorphism, and simple sequence repeat markers, were used. Additionally, DNA markers were developed based on disease resistance gene homologs identified from the genome sequence of <i>N. benthamiana</i>. Linkage analysis revealed that <i>HLA1</i> was located between two cleaved amplified polymorphic sequence markers Nb14-CAPS and NbRGH1-CAPS at a distance of 10.8 and 10.9 cM, respectively. The distance between these markers was equivalent to a 682 kb interval in the genome sequence of <i>N. benthamiana</i>. |
| first_indexed | 2024-03-10T06:15:07Z |
| format | Article |
| id | doaj.art-40f3fbfcb8824ad999bb209c1645c01f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:07Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-40f3fbfcb8824ad999bb209c1645c01f2023-11-22T19:43:10ZengMDPI AGPlants2223-77472021-09-011010206210.3390/plants10102062Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific HybridsTakahiro Tezuka0Naoto Kitamura1Sae Imagawa2Akira Hasegawa3Kumpei Shiragaki4Hai He5Masanori Yanase6Yoshiyuki Ogata7Toshinobu Morikawa8Shuji Yokoi9Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanSchool of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanSchool of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, JapanHybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F<sub>1</sub> hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus <i>Nicotiana</i>, <i>N. debneyi</i> has the dominant allele <i>Hla1-1</i> at the <i>HLA1</i> locus that causes hybrid lethality in F<sub>1</sub> hybrid seedlings by interaction with <i>N. tabacum</i> allele(s). Here, we mapped the <i>HLA1</i> locus using the F<sub>2</sub> population segregating for the <i>Hla1-1</i> allele derived from the interspecific cross between <i>N. debneyi</i> and <i>N. fragrans</i>. To map <i>HLA1</i>, several DNA markers including random amplified polymorphic DNA, amplified fragment length polymorphism, and simple sequence repeat markers, were used. Additionally, DNA markers were developed based on disease resistance gene homologs identified from the genome sequence of <i>N. benthamiana</i>. Linkage analysis revealed that <i>HLA1</i> was located between two cleaved amplified polymorphic sequence markers Nb14-CAPS and NbRGH1-CAPS at a distance of 10.8 and 10.9 cM, respectively. The distance between these markers was equivalent to a 682 kb interval in the genome sequence of <i>N. benthamiana</i>.https://www.mdpi.com/2223-7747/10/10/2062hybrid lethalityinterspecific populationlinkage analysis<i>Nicotiana debneyi</i><i>Nicotiana fragrans</i>reproductive isolation |
| spellingShingle | Takahiro Tezuka Naoto Kitamura Sae Imagawa Akira Hasegawa Kumpei Shiragaki Hai He Masanori Yanase Yoshiyuki Ogata Toshinobu Morikawa Shuji Yokoi Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids Plants hybrid lethality interspecific population linkage analysis <i>Nicotiana debneyi</i> <i>Nicotiana fragrans</i> reproductive isolation |
| title | Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids |
| title_full | Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids |
| title_fullStr | Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids |
| title_full_unstemmed | Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids |
| title_short | Genetic Mapping of the <i>HLA1</i> Locus Causing Hybrid Lethality in <i>Nicotiana</i> Interspecific Hybrids |
| title_sort | genetic mapping of the i hla1 i locus causing hybrid lethality in i nicotiana i interspecific hybrids |
| topic | hybrid lethality interspecific population linkage analysis <i>Nicotiana debneyi</i> <i>Nicotiana fragrans</i> reproductive isolation |
| url | https://www.mdpi.com/2223-7747/10/10/2062 |
| work_keys_str_mv | AT takahirotezuka geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT naotokitamura geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT saeimagawa geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT akirahasegawa geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT kumpeishiragaki geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT haihe geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT masanoriyanase geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT yoshiyukiogata geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT toshinobumorikawa geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids AT shujiyokoi geneticmappingoftheihla1ilocuscausinghybridlethalityininicotianaiinterspecifichybrids |