Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts
Background/purpose: Cigarette smoking is an important risk factor in the pathogenesis of periodontal disease. However, little is known about the effect of nicotine, the major component of cigarette smoke, on cementoblasts. The aim of this study was to investigate the pathological effects of nicotine...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790214000403 |
| _version_ | 1819296073610428416 |
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| author | Chun-San Chen Shiuan-Shinn Lee Hui-Chieh Yu Fu-Mei Huang Yu-Chao Chang |
| author_facet | Chun-San Chen Shiuan-Shinn Lee Hui-Chieh Yu Fu-Mei Huang Yu-Chao Chang |
| author_sort | Chun-San Chen |
| collection | DOAJ |
| description | Background/purpose: Cigarette smoking is an important risk factor in the pathogenesis of periodontal disease. However, little is known about the effect of nicotine, the major component of cigarette smoke, on cementoblasts. The aim of this study was to investigate the pathological effects of nicotine on the murine immortalized cementoblast cell line (OCCM.30).
Materials and methods: Cell viability was judged by using the Alamar Blue reduction assay. Cell migration was evaluated by transwell and wound-healing assays. The protein concentrations of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were measured by using enzyme linked immunosorbent assay (ELISA). The semiquantitative 2′,7′-dichlorfluorescein-diacetate (DCFH-DA) fluorescence technique was used to detect the intracellular level of reactive oxygen species (ROS).
Results: Concentrations of nicotine > 1.5mM demonstrated cytotoxicity to cementoblasts (P < 0.05). Nicotine attenuated cell migration in a dose-dependent manner (P < 0.05). In addition, nicotine augmented the production of IL-6 and TNF-α in a dose-dependent manner (P < 0.05). The concentration of 1mM nicotine enhanced the generation of intracellular ROS in a time-dependent manner (P < 0.05).
Conclusion: Taken together, these results suggest that nicotine could inhibit the growth and migration of cementoblasts. In addition, nicotine could also induce the generation of inflammatory cytokines and ROS by cementoblasts. |
| first_indexed | 2024-12-24T04:52:19Z |
| format | Article |
| id | doaj.art-40f4ebf4384a497cb0fac39fffc13cf9 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-24T04:52:19Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-40f4ebf4384a497cb0fac39fffc13cf92022-12-21T17:14:30ZengElsevierJournal of Dental Sciences1991-79022015-06-0110215416010.1016/j.jds.2014.04.002Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblastsChun-San Chen0Shiuan-Shinn Lee1Hui-Chieh Yu2Fu-Mei Huang3Yu-Chao Chang4School of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Public Health, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanBackground/purpose: Cigarette smoking is an important risk factor in the pathogenesis of periodontal disease. However, little is known about the effect of nicotine, the major component of cigarette smoke, on cementoblasts. The aim of this study was to investigate the pathological effects of nicotine on the murine immortalized cementoblast cell line (OCCM.30). Materials and methods: Cell viability was judged by using the Alamar Blue reduction assay. Cell migration was evaluated by transwell and wound-healing assays. The protein concentrations of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were measured by using enzyme linked immunosorbent assay (ELISA). The semiquantitative 2′,7′-dichlorfluorescein-diacetate (DCFH-DA) fluorescence technique was used to detect the intracellular level of reactive oxygen species (ROS). Results: Concentrations of nicotine > 1.5mM demonstrated cytotoxicity to cementoblasts (P < 0.05). Nicotine attenuated cell migration in a dose-dependent manner (P < 0.05). In addition, nicotine augmented the production of IL-6 and TNF-α in a dose-dependent manner (P < 0.05). The concentration of 1mM nicotine enhanced the generation of intracellular ROS in a time-dependent manner (P < 0.05). Conclusion: Taken together, these results suggest that nicotine could inhibit the growth and migration of cementoblasts. In addition, nicotine could also induce the generation of inflammatory cytokines and ROS by cementoblasts.http://www.sciencedirect.com/science/article/pii/S1991790214000403cementoblastscytotoxicityinflammatory cytokinesmigrationnicotineROS generation |
| spellingShingle | Chun-San Chen Shiuan-Shinn Lee Hui-Chieh Yu Fu-Mei Huang Yu-Chao Chang Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts Journal of Dental Sciences cementoblasts cytotoxicity inflammatory cytokines migration nicotine ROS generation |
| title | Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| title_full | Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| title_fullStr | Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| title_full_unstemmed | Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| title_short | Effects of nicotine on cell growth, migration, and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| title_sort | effects of nicotine on cell growth migration and production of inflammatory cytokines and reactive oxygen species by cementoblasts |
| topic | cementoblasts cytotoxicity inflammatory cytokines migration nicotine ROS generation |
| url | http://www.sciencedirect.com/science/article/pii/S1991790214000403 |
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