Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange
The aim of the presented work was to assess the potential of Medicago polymorpha extract to synthesize silver nanoparticles (AgNPs) as a green method. It was a simple one-step synthesis approach and the product obtained was characterized by UV-visible spectroscopy, Fourier transform infrared (FTIR),...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2019-01-01
|
| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2018-0030 |
| _version_ | 1818722095891218432 |
|---|---|
| author | Ismail Muhammad Gul Saima Khan M.I. Khan Murad Ali Asiri Abdullah M. Khan Sher Bahadar |
| author_facet | Ismail Muhammad Gul Saima Khan M.I. Khan Murad Ali Asiri Abdullah M. Khan Sher Bahadar |
| author_sort | Ismail Muhammad |
| collection | DOAJ |
| description | The aim of the presented work was to assess the potential of Medicago polymorpha extract to synthesize silver nanoparticles (AgNPs) as a green method. It was a simple one-step synthesis approach and the product obtained was characterized by UV-visible spectroscopy, Fourier transform infrared (FTIR), powder X-ray diffraction, thermogravimetric analysis, and field-emission scanning electron microscopy (FE-SEM). At room temperature, the optimum time for the completion of the reaction (i.e. the formation colloidal solution) was just 5 min. FE-SEM images showed that AgNPs were predominantly in spheres, whereas FTIR spectrum analysis inferred that gallic acid present in the extract initially reduced silver ions to elemental silver. The carboxylic and hydroxyl groups of biomolecules present in the extract stabilized AgNPs by passivating the surface to prevent aggregation, resulting in uniform distribution. The antibacterial activity of synthesized AgNPs showed effective inhibitory effects against waterborne pathogens, including Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), at a minimum inhibitory concentration of 10 μg/ml. Membrane permeability and respiration studies were also performed to assess the surface role of the synthesized AgNPs. The prepared AgNPs exhibited excellent antioxidant activity and catalytic reduction of methyl orange with a rate constant of 6.8×10−3 s−1. |
| first_indexed | 2024-12-17T20:49:11Z |
| format | Article |
| id | doaj.art-40f51f5701df42a881284282e9722a80 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-12-17T20:49:11Z |
| publishDate | 2019-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-40f51f5701df42a881284282e9722a802022-12-21T21:33:04ZengDe GruyterGreen Processing and Synthesis2191-95502019-01-018111812710.1515/gps-2018-0030gps-2018-0030Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orangeIsmail Muhammad0Gul Saima1Khan M.I.2Khan Murad Ali3Asiri Abdullah M.4Khan Sher Bahadar5Department of Chemistry, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, PakistanDepartment of Chemistry, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, PakistanDepartment of Chemistry, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, PakistanDepartment of Chemistry, Kohat University of Science and Technology Kohat-26000, Khyber Pakhtunkhwa, PakistanCenter of Excellence for Advanced Materials Research and Chemistry Department, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi ArabiaCenter of Excellence for Advanced Materials Research and Chemistry Department, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi ArabiaThe aim of the presented work was to assess the potential of Medicago polymorpha extract to synthesize silver nanoparticles (AgNPs) as a green method. It was a simple one-step synthesis approach and the product obtained was characterized by UV-visible spectroscopy, Fourier transform infrared (FTIR), powder X-ray diffraction, thermogravimetric analysis, and field-emission scanning electron microscopy (FE-SEM). At room temperature, the optimum time for the completion of the reaction (i.e. the formation colloidal solution) was just 5 min. FE-SEM images showed that AgNPs were predominantly in spheres, whereas FTIR spectrum analysis inferred that gallic acid present in the extract initially reduced silver ions to elemental silver. The carboxylic and hydroxyl groups of biomolecules present in the extract stabilized AgNPs by passivating the surface to prevent aggregation, resulting in uniform distribution. The antibacterial activity of synthesized AgNPs showed effective inhibitory effects against waterborne pathogens, including Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), at a minimum inhibitory concentration of 10 μg/ml. Membrane permeability and respiration studies were also performed to assess the surface role of the synthesized AgNPs. The prepared AgNPs exhibited excellent antioxidant activity and catalytic reduction of methyl orange with a rate constant of 6.8×10−3 s−1.https://doi.org/10.1515/gps-2018-0030biogenic silver nanoparticlesdye removalmembrane permeability |
| spellingShingle | Ismail Muhammad Gul Saima Khan M.I. Khan Murad Ali Asiri Abdullah M. Khan Sher Bahadar Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange Green Processing and Synthesis biogenic silver nanoparticles dye removal membrane permeability |
| title | Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| title_full | Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| title_fullStr | Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| title_full_unstemmed | Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| title_short | Medicago polymorpha-mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| title_sort | medicago polymorpha mediated antibacterial silver nanoparticles in the reduction of methyl orange |
| topic | biogenic silver nanoparticles dye removal membrane permeability |
| url | https://doi.org/10.1515/gps-2018-0030 |
| work_keys_str_mv | AT ismailmuhammad medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange AT gulsaima medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange AT khanmi medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange AT khanmuradali medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange AT asiriabdullahm medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange AT khansherbahadar medicagopolymorphamediatedantibacterialsilvernanoparticlesinthereductionofmethylorange |