Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus
Background: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific polyclonal antisera. Results: Out of 18 genotypes,...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722004840 |
| _version_ | 1811255589689360384 |
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| author | Sehrish Saba Muhammad Ashfaq Mirza Abid Mehmood Furqan Ahmad Saleh Alfarraj Mohammad Javed Ansari Amir Maqbool |
| author_facet | Sehrish Saba Muhammad Ashfaq Mirza Abid Mehmood Furqan Ahmad Saleh Alfarraj Mohammad Javed Ansari Amir Maqbool |
| author_sort | Sehrish Saba |
| collection | DOAJ |
| description | Background: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific polyclonal antisera. Results: Out of 18 genotypes, four genotypes viz., Loungi pepper, Sanam, Red Pepper, and CV-1 were categorized as highly susceptible followed by three susceptible (Soofi, Royal Wonder, Pepper PLSQ) and four (Yolo wonder, Bhakkar Local, CIM-506, CR-25) were moderately susceptible. The plants of these genotypes exhibited characteristic symptoms of ChiVMV and accumulated a high level of virus titer. The rest of three (California wonder, Gola Peshawar, Red top) and four (CV-7, Hot Pepper, Doni Pepper, Ghotki) genotypes were grouped as resistant and moderately resistant, respectively. Three resistant and three highly susceptible genotypes were further appraised for genetic diversity by RAPD markers and a total of 75 bands were scored, 76% of them unveiled polymorphism with an amplification range of 5–9 bands. Genetic analysis revealed 44–78.67% similarities with Mean Similarity Index (MSI) ranging from 59.46 to 65.86%. Most of the primers were highly informative with PIC value fluctuated from 0.388 to 0.666, with an average value of 0.512. Cluster analysis further confirmed and clustered both groups of genotypes in a separate clade. Conclusion: It is concluded that genotypes; California Wonder and Red Top showed diverse response from other genotypes, not only based on symptoms and ELISA but also on markers' basis. Therefore, these genotypes could be useful as ChiVMV resistant sources in the chilli breeding program. |
| first_indexed | 2024-04-12T17:27:13Z |
| format | Article |
| id | doaj.art-40f703ce572f4773af6c9c14b4a386f3 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T17:27:13Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-40f703ce572f4773af6c9c14b4a386f32022-12-22T03:23:16ZengElsevierJournal of King Saud University: Science1018-36472022-11-01348102303Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virusSehrish Saba0Muhammad Ashfaq1Mirza Abid Mehmood2Furqan Ahmad3Saleh Alfarraj4Mohammad Javed Ansari5Amir Maqbool6Department of Plant Pathology, PMAS-Arid Agriculture University Rawalpindi, PakistanDepartment of Plant Pathology, PMAS-Arid Agriculture University Rawalpindi, Pakistan; Plant Pathology, IPP, MNS-University of Agriculture, Multan, Pakistan; Corresponding author at: Plant Pathology, IPP, MNS-University of Agriculture, Multan, Pakistan.Plant Pathology, IPP, MNS-University of Agriculture, Multan, PakistanInstitute of Plant Breeding and Biotechnology, MNS-University of Agriculture, Multan, PakistanZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany, Hindu College Moradabad (Mahatma Jyotiba Phule Rohilkhand University Bareilly), 244001, IndiaDepartment of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, Nigde, Turkey; Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, 91198 Gif-sur-Yvette, FranceBackground: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific polyclonal antisera. Results: Out of 18 genotypes, four genotypes viz., Loungi pepper, Sanam, Red Pepper, and CV-1 were categorized as highly susceptible followed by three susceptible (Soofi, Royal Wonder, Pepper PLSQ) and four (Yolo wonder, Bhakkar Local, CIM-506, CR-25) were moderately susceptible. The plants of these genotypes exhibited characteristic symptoms of ChiVMV and accumulated a high level of virus titer. The rest of three (California wonder, Gola Peshawar, Red top) and four (CV-7, Hot Pepper, Doni Pepper, Ghotki) genotypes were grouped as resistant and moderately resistant, respectively. Three resistant and three highly susceptible genotypes were further appraised for genetic diversity by RAPD markers and a total of 75 bands were scored, 76% of them unveiled polymorphism with an amplification range of 5–9 bands. Genetic analysis revealed 44–78.67% similarities with Mean Similarity Index (MSI) ranging from 59.46 to 65.86%. Most of the primers were highly informative with PIC value fluctuated from 0.388 to 0.666, with an average value of 0.512. Cluster analysis further confirmed and clustered both groups of genotypes in a separate clade. Conclusion: It is concluded that genotypes; California Wonder and Red Top showed diverse response from other genotypes, not only based on symptoms and ELISA but also on markers' basis. Therefore, these genotypes could be useful as ChiVMV resistant sources in the chilli breeding program.http://www.sciencedirect.com/science/article/pii/S1018364722004840ChilliRAPDGenetic diversityELISAChiVMV |
| spellingShingle | Sehrish Saba Muhammad Ashfaq Mirza Abid Mehmood Furqan Ahmad Saleh Alfarraj Mohammad Javed Ansari Amir Maqbool Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus Journal of King Saud University: Science Chilli RAPD Genetic diversity ELISA ChiVMV |
| title | Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| title_full | Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| title_fullStr | Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| title_full_unstemmed | Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| title_short | Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| title_sort | sero rapd makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus |
| topic | Chilli RAPD Genetic diversity ELISA ChiVMV |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722004840 |
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