Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components
Flammulina filiformis (F. filiformis) is one of the four major edible types of fungus in the world and has been cultivated in China since 800 CE (Anno Domini). Some of the most essential criteria for evaluating the quality of F. filiformis are the types and contents of volatile components present. A...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566222000843 |
| _version_ | 1797802099034030080 |
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| author | Huan Lu Wei Song Xiao-Dong Shang Jian-Yu Liu Dan Zhang Liang Li Rui-Juan Wang Xiao-Ting Zhai Tao Feng |
| author_facet | Huan Lu Wei Song Xiao-Dong Shang Jian-Yu Liu Dan Zhang Liang Li Rui-Juan Wang Xiao-Ting Zhai Tao Feng |
| author_sort | Huan Lu |
| collection | DOAJ |
| description | Flammulina filiformis (F. filiformis) is one of the four major edible types of fungus in the world and has been cultivated in China since 800 CE (Anno Domini). Some of the most essential criteria for evaluating the quality of F. filiformis are the types and contents of volatile components present. A focused study on screened the terpene synthase genes involved in the aroma of offspring and compared key terpenoids between parents and offspring, which is helpful for the development and application of F. filiformis. Firstly, the volatile aroma components of parent and offspring F. filiformis were extracted using two pretreatment procedures, and then were semi-quantified by an internal standard. Forty-eight, fifty-eight, and forty-eight volatile compounds were identified in parents and offspring of three different strains, and 15, 22, and 12 aroma compounds (OAVs ≥ 1) were further screened out via calculating their odor activity values (OAVs). Terpenoids, in particular linalool and eucalyptol, which contribute more to the aroma, result in the unique green and grassy aroma of the offspring. At last, the F. filiformis genome was resequenced and the coordinates of genes related to terpenoid synthase were determined. The results showed that Scaffolds, including scaffold3.t874 and scaffold9.t157 were connected to terpenoid synthesis of offspring (No. 61523). The variant genes g269 and g61 were related to terpenoid synthase sequences. This study provides a theoretical foundation for the cultivation of more diverse and unique varieties of F. filiformis. |
| first_indexed | 2024-03-13T05:00:32Z |
| format | Article |
| id | doaj.art-40fa949d4f1449f8a4231744b81d6423 |
| institution | Directory Open Access Journal |
| issn | 2666-5662 |
| language | English |
| last_indexed | 2024-03-13T05:00:32Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj.art-40fa949d4f1449f8a4231744b81d64232023-06-17T05:21:15ZengElsevierFood Chemistry: Molecular Sciences2666-56622023-07-016100156Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma componentsHuan Lu0Wei Song1Xiao-Dong Shang2Jian-Yu Liu3Dan Zhang4Liang Li5Rui-Juan Wang6Xiao-Ting Zhai7Tao Feng8National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, China; Corresponding author.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaFlammulina filiformis (F. filiformis) is one of the four major edible types of fungus in the world and has been cultivated in China since 800 CE (Anno Domini). Some of the most essential criteria for evaluating the quality of F. filiformis are the types and contents of volatile components present. A focused study on screened the terpene synthase genes involved in the aroma of offspring and compared key terpenoids between parents and offspring, which is helpful for the development and application of F. filiformis. Firstly, the volatile aroma components of parent and offspring F. filiformis were extracted using two pretreatment procedures, and then were semi-quantified by an internal standard. Forty-eight, fifty-eight, and forty-eight volatile compounds were identified in parents and offspring of three different strains, and 15, 22, and 12 aroma compounds (OAVs ≥ 1) were further screened out via calculating their odor activity values (OAVs). Terpenoids, in particular linalool and eucalyptol, which contribute more to the aroma, result in the unique green and grassy aroma of the offspring. At last, the F. filiformis genome was resequenced and the coordinates of genes related to terpenoid synthase were determined. The results showed that Scaffolds, including scaffold3.t874 and scaffold9.t157 were connected to terpenoid synthesis of offspring (No. 61523). The variant genes g269 and g61 were related to terpenoid synthase sequences. This study provides a theoretical foundation for the cultivation of more diverse and unique varieties of F. filiformis.http://www.sciencedirect.com/science/article/pii/S2666566222000843Flammulina filiformisHS-SPMESAFEAroma profilesTerpenoidsResequencing |
| spellingShingle | Huan Lu Wei Song Xiao-Dong Shang Jian-Yu Liu Dan Zhang Liang Li Rui-Juan Wang Xiao-Ting Zhai Tao Feng Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components Food Chemistry: Molecular Sciences Flammulina filiformis HS-SPME SAFE Aroma profiles Terpenoids Resequencing |
| title | Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components |
| title_full | Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components |
| title_fullStr | Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components |
| title_full_unstemmed | Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components |
| title_short | Expression of terpene synthase-related genes in parents and offspring of Flammulina filiformis based on differences in volatile aroma components |
| title_sort | expression of terpene synthase related genes in parents and offspring of flammulina filiformis based on differences in volatile aroma components |
| topic | Flammulina filiformis HS-SPME SAFE Aroma profiles Terpenoids Resequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2666566222000843 |
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