Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes
The plant growth regulator (PGR) effects on callus induction frequency (CIF) and plantlet regeneration were studied on four rice genotypes. Mature seeds were used as explant for callus induction in different combinations of 2, 4-D, Kn and NAA using MS and N6 medium. The maximum CIF recorded was 86...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2023-03-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4611 |
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| author | P. Sharmela1, N. Meenakshi ganesan1*, R. Saraswathi2, R. Gnanam3 and C. N. Chandrasekhar4 |
| author_facet | P. Sharmela1, N. Meenakshi ganesan1*, R. Saraswathi2, R. Gnanam3 and C. N. Chandrasekhar4 |
| author_sort | P. Sharmela1, N. Meenakshi ganesan1*, R. Saraswathi2, R. Gnanam3 and C. N. Chandrasekhar4 |
| collection | DOAJ |
| description | The plant growth regulator (PGR) effects on callus induction frequency (CIF) and plantlet regeneration were studied
on four rice genotypes. Mature seeds were used as explant for callus induction in different combinations of 2, 4-D,
Kn and NAA using MS and N6 medium. The maximum CIF recorded was 86.0% (Azucena) and 83.3% (Palwan)
in N6 media augmented with 2,4-D, Kn and NAA at 2.5, 0.5 and 1.0 mg/L, respectively. In genotypes CB174R and
CB87R, 66.7% and 71.6% CIF were obtained in N6 media supplemented with 2,4-D, Kn and NAA at 2.0, 0.25 and 0.5
mg/L, respectively. In MS medium a total plantlet regeneration of 70% was observed in Palwan and 10% in CB174R
augmented with NAA (0.5 mg/L) + BAP (1.0 mg/L) + Kn (0.5 mg/L) and NAA (0.5, 1.0 mg/L) + BAP (0.5, 1.0 mg/L) +
Kn (0.5, 2.0 mg/L), respectively. The efficiency of plantlet regeneration depended on genotype, media constituents
and PGR. |
| first_indexed | 2024-04-09T18:24:08Z |
| format | Article |
| id | doaj.art-40fbeba1a9cb4eda8f24b890795409d4 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-04-09T18:24:08Z |
| publishDate | 2023-03-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-40fbeba1a9cb4eda8f24b890795409d42023-04-12T05:20:16ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2023-03-01141314210.37992/2023.1401.012Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypesP. Sharmela1, N. Meenakshi ganesan1*, R. Saraswathi2, R. Gnanam3 and C. N. Chandrasekhar401Department of Genetics and Plant Breeding, TNAU, Coimbatore, Tamil Nadu, India. 2Department of Plant Genetic Resources, TNAU, Coimbatore, Tamil Nadu, India. 3Department of Plant Molecular Biology and Bioinformatics, TNAU, Coimbatore, Tamil Nadu, India. 4Directorate of Open and Distance Learning, TNAU, Coimbatore, Tamil Nadu, India. *E-Mail: meenakshignsncbe@gmail.comThe plant growth regulator (PGR) effects on callus induction frequency (CIF) and plantlet regeneration were studied on four rice genotypes. Mature seeds were used as explant for callus induction in different combinations of 2, 4-D, Kn and NAA using MS and N6 medium. The maximum CIF recorded was 86.0% (Azucena) and 83.3% (Palwan) in N6 media augmented with 2,4-D, Kn and NAA at 2.5, 0.5 and 1.0 mg/L, respectively. In genotypes CB174R and CB87R, 66.7% and 71.6% CIF were obtained in N6 media supplemented with 2,4-D, Kn and NAA at 2.0, 0.25 and 0.5 mg/L, respectively. In MS medium a total plantlet regeneration of 70% was observed in Palwan and 10% in CB174R augmented with NAA (0.5 mg/L) + BAP (1.0 mg/L) + Kn (0.5 mg/L) and NAA (0.5, 1.0 mg/L) + BAP (0.5, 1.0 mg/L) + Kn (0.5, 2.0 mg/L), respectively. The efficiency of plantlet regeneration depended on genotype, media constituents and PGR.https://www.ejplantbreeding.org/index.php/EJPB/article/view/4611riceembryo culturecallus inductionregenerationcallogenesisrhizogenesis |
| spellingShingle | P. Sharmela1, N. Meenakshi ganesan1*, R. Saraswathi2, R. Gnanam3 and C. N. Chandrasekhar4 Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes Electronic Journal of Plant Breeding rice embryo culture callus induction regeneration callogenesis rhizogenesis |
| title | Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes |
| title_full | Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes |
| title_fullStr | Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes |
| title_full_unstemmed | Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes |
| title_short | Optimization of in vitro culture system in tropical Japonica and Indica rice (Oryza sativa L.) genotypes |
| title_sort | optimization of in vitro culture system in tropical japonica and indica rice oryza sativa l genotypes |
| topic | rice embryo culture callus induction regeneration callogenesis rhizogenesis |
| url | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4611 |
| work_keys_str_mv | AT psharmela1nmeenakshiganesan1rsaraswathi2rgnanam3andcnchandrasekhar4 optimizationofinvitroculturesystemintropicaljaponicaandindicariceoryzasativalgenotypes |