Donkey Epididymal Transport for Semen Cooling and Freezing
The objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, <i>n</i> = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, <i>n</i> = 14) or dissec...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/10/12/2209 |
| _version_ | 1797546763972771840 |
|---|---|
| author | Yamilka Lago-Alvarez Giorgia Podico Lorenzo G. Segabinazzi Lais L. Cunha Leonardo Barbosa Carolyn E. Arnold Fabio S. Lima Luise T. King Amy K. McLean Igor F. Canisso |
| author_facet | Yamilka Lago-Alvarez Giorgia Podico Lorenzo G. Segabinazzi Lais L. Cunha Leonardo Barbosa Carolyn E. Arnold Fabio S. Lima Luise T. King Amy K. McLean Igor F. Canisso |
| author_sort | Yamilka Lago-Alvarez |
| collection | DOAJ |
| description | The objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, <i>n</i> = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, <i>n</i> = 14) or dissected apart (Experiment 3, <i>n</i> = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP). Data were analyzed with R using mixed models and Tukey’s test as posthoc. Results showed that the cooling of epididymal semen up to 24 h after harvesting did not affect motility parameters or plasma membrane integrity; furthermore, in Experiment 3, the post-thaw evaluation of both Centrif and Non-Centrif achieved similar TM and PM. Collectively, the post-thaw results revealed low motility parameters across groups; while, the PMI and HMMP did not reflect this trend, and the values remained high, suggesting that there was a lack of epididymal sperm activation with either centrifugation or extenders. In summary, freshly harvested and cooled-shipped and cooled semen had satisfactory semen parameters. Future studies need to address donkey epididymal semen fertility in mares and jennies. |
| first_indexed | 2024-03-10T14:34:46Z |
| format | Article |
| id | doaj.art-40fc212a1bfb4ea68b16cbde37f2ead3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:34:46Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-40fc212a1bfb4ea68b16cbde37f2ead32023-11-20T22:18:01ZengMDPI AGAnimals2076-26152020-11-011012220910.3390/ani10122209Donkey Epididymal Transport for Semen Cooling and FreezingYamilka Lago-Alvarez0Giorgia Podico1Lorenzo G. Segabinazzi2Lais L. Cunha3Leonardo Barbosa4Carolyn E. Arnold5Fabio S. Lima6Luise T. King7Amy K. McLean8Igor F. Canisso9Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77840, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USADepartment of Veterinary Clinical Medicine, University of Arizona, Oro Valley, AZ 85704, USADepartment of Animal Sciences, University of California, Davis, CA 95161, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801, USAThe objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, <i>n</i> = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, <i>n</i> = 14) or dissected apart (Experiment 3, <i>n</i> = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP). Data were analyzed with R using mixed models and Tukey’s test as posthoc. Results showed that the cooling of epididymal semen up to 24 h after harvesting did not affect motility parameters or plasma membrane integrity; furthermore, in Experiment 3, the post-thaw evaluation of both Centrif and Non-Centrif achieved similar TM and PM. Collectively, the post-thaw results revealed low motility parameters across groups; while, the PMI and HMMP did not reflect this trend, and the values remained high, suggesting that there was a lack of epididymal sperm activation with either centrifugation or extenders. In summary, freshly harvested and cooled-shipped and cooled semen had satisfactory semen parameters. Future studies need to address donkey epididymal semen fertility in mares and jennies.https://www.mdpi.com/2076-2615/10/12/2209epididymiscooled-shipped epididymidescastrationtestissemen cryopreservation |
| spellingShingle | Yamilka Lago-Alvarez Giorgia Podico Lorenzo G. Segabinazzi Lais L. Cunha Leonardo Barbosa Carolyn E. Arnold Fabio S. Lima Luise T. King Amy K. McLean Igor F. Canisso Donkey Epididymal Transport for Semen Cooling and Freezing Animals epididymis cooled-shipped epididymides castration testis semen cryopreservation |
| title | Donkey Epididymal Transport for Semen Cooling and Freezing |
| title_full | Donkey Epididymal Transport for Semen Cooling and Freezing |
| title_fullStr | Donkey Epididymal Transport for Semen Cooling and Freezing |
| title_full_unstemmed | Donkey Epididymal Transport for Semen Cooling and Freezing |
| title_short | Donkey Epididymal Transport for Semen Cooling and Freezing |
| title_sort | donkey epididymal transport for semen cooling and freezing |
| topic | epididymis cooled-shipped epididymides castration testis semen cryopreservation |
| url | https://www.mdpi.com/2076-2615/10/12/2209 |
| work_keys_str_mv | AT yamilkalagoalvarez donkeyepididymaltransportforsemencoolingandfreezing AT giorgiapodico donkeyepididymaltransportforsemencoolingandfreezing AT lorenzogsegabinazzi donkeyepididymaltransportforsemencoolingandfreezing AT laislcunha donkeyepididymaltransportforsemencoolingandfreezing AT leonardobarbosa donkeyepididymaltransportforsemencoolingandfreezing AT carolynearnold donkeyepididymaltransportforsemencoolingandfreezing AT fabioslima donkeyepididymaltransportforsemencoolingandfreezing AT luisetking donkeyepididymaltransportforsemencoolingandfreezing AT amykmclean donkeyepididymaltransportforsemencoolingandfreezing AT igorfcanisso donkeyepididymaltransportforsemencoolingandfreezing |