PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process
The challenges of formulating recombinant human growth hormone (rhGH) into sustained-release polymeric microspheres include two mutual causal factors, protein denaturing by the formulation process and severe initial burst release related with relative high dose. The stabilizers to protect the protei...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/7/683 |
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| author | Jebun Nessa Diana Ying Tao Qiran Du Meng Wang Chinta Uday Kumar Fei Wu Tuo Jin |
| author_facet | Jebun Nessa Diana Ying Tao Qiran Du Meng Wang Chinta Uday Kumar Fei Wu Tuo Jin |
| author_sort | Jebun Nessa Diana |
| collection | DOAJ |
| description | The challenges of formulating recombinant human growth hormone (rhGH) into sustained-release polymeric microspheres include two mutual causal factors, protein denaturing by the formulation process and severe initial burst release related with relative high dose. The stabilizers to protect the proteins must not evoke osmotic pressure inside the microspheres, and the contact of the protein with the interface between water and organic solution of the polymer must be minimized. To meet these criteria, rhGH was pre-formulated into polysaccharide particles via an aqueous–aqueous emulsion in the present study, followed by encapsulating the particles into microspheres through a self-regulated process to minimize the contact of the protein with the water–oil interface. Polysaccharides as the protein stabilizer did not evoke osmotic pressure as small sugar stabilizers, the cause of severe initial burst release. Reduced initial burst enabled reduced protein loading to 9% (from 22% of the once commercialized Nutropin depot), which in turn reduced the dosage form index from 80 to 8.7 and eased the initial burst. A series of physical chemical characterizations as well as biologic and pharmacokinetic assays confirmed that the present method is practically feasible for preparing microspheres of proteins. |
| first_indexed | 2024-03-10T18:21:42Z |
| format | Article |
| id | doaj.art-40fd261a1eb14bcab0a09aac72b2dcd3 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T18:21:42Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-40fd261a1eb14bcab0a09aac72b2dcd32023-11-20T07:17:34ZengMDPI AGPharmaceutics1999-49232020-07-0112768310.3390/pharmaceutics12070683PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated ProcessJebun Nessa Diana0Ying Tao1Qiran Du2Meng Wang3Chinta Uday Kumar4Fei Wu5Tuo Jin6School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaThe challenges of formulating recombinant human growth hormone (rhGH) into sustained-release polymeric microspheres include two mutual causal factors, protein denaturing by the formulation process and severe initial burst release related with relative high dose. The stabilizers to protect the proteins must not evoke osmotic pressure inside the microspheres, and the contact of the protein with the interface between water and organic solution of the polymer must be minimized. To meet these criteria, rhGH was pre-formulated into polysaccharide particles via an aqueous–aqueous emulsion in the present study, followed by encapsulating the particles into microspheres through a self-regulated process to minimize the contact of the protein with the water–oil interface. Polysaccharides as the protein stabilizer did not evoke osmotic pressure as small sugar stabilizers, the cause of severe initial burst release. Reduced initial burst enabled reduced protein loading to 9% (from 22% of the once commercialized Nutropin depot), which in turn reduced the dosage form index from 80 to 8.7 and eased the initial burst. A series of physical chemical characterizations as well as biologic and pharmacokinetic assays confirmed that the present method is practically feasible for preparing microspheres of proteins.https://www.mdpi.com/1999-4923/12/7/683recombinant human growth hormone (rhGH)sustained release microspheresprotein aggregationstability |
| spellingShingle | Jebun Nessa Diana Ying Tao Qiran Du Meng Wang Chinta Uday Kumar Fei Wu Tuo Jin PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process Pharmaceutics recombinant human growth hormone (rhGH) sustained release microspheres protein aggregation stability |
| title | PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process |
| title_full | PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process |
| title_fullStr | PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process |
| title_full_unstemmed | PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process |
| title_short | PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process |
| title_sort | plga microspheres of hgh of preserved native state prepared using a self regulated process |
| topic | recombinant human growth hormone (rhGH) sustained release microspheres protein aggregation stability |
| url | https://www.mdpi.com/1999-4923/12/7/683 |
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