Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications
In this study, an aqueous extract of Gracilaria crassa and Grateloupia lithophila was used for the green synthesis of silver nanoparticles (AgNPs) to control plant disease and support plant growth. The synthesised AgNPs were characterised using a UV–visible spectrophotometer, FTIR, XRD, SEM, EDAX, T...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154323002764 |
| _version_ | 1797385077768847360 |
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| author | Thynraj Antony Roseline Muthiyal Prabakaran Sudhakar Kulanthaiyesu Arunkumar |
| author_facet | Thynraj Antony Roseline Muthiyal Prabakaran Sudhakar Kulanthaiyesu Arunkumar |
| author_sort | Thynraj Antony Roseline |
| collection | DOAJ |
| description | In this study, an aqueous extract of Gracilaria crassa and Grateloupia lithophila was used for the green synthesis of silver nanoparticles (AgNPs) to control plant disease and support plant growth. The synthesised AgNPs were characterised using a UV–visible spectrophotometer, FTIR, XRD, SEM, EDAX, TEM and DLS analysis. The TEM image revealed that synthesised nanoparticles with average size of 51 nm and 20 nm were observed in G. crassa and Grateloupia lithophila extract, respectively. The green synthesised AgNPs exhibited a strong antibacterial activity against Xanthomonas axonopodis pv. citri and Xanthomonas oryzae pv. oryzae, and investigated the effect of biogenic AgNPs on seed germination and seedling growth of rice plants (Oryzae sativa L). Both the sizes (51 and 20 nm) of AgNPs treatments (50 μg/ml conc.) resulted in the highest seed germination rate (98 and 99%), longer roots (17.51 ± 0.50 and 17.68 ± 0.10 cm) and shoot lengths (5.68 ± 0.12 and 6.18 ± 0.10 cm), and produced higher amount of fresh (8.98 ± 0.34 and 9.00 ± 0.14 mg) and dry (3.68 ± 0.05 and 3.80 ± 0.12 mg) biomass weights. On the other hand, rice seedlings treated with both sizes of AgNPs, particularly at 100 μg/ml concentration, responded with a lower rate of seed germination, shorter root and shoot length and a lower amount of fresh and dry biomass weight was observed. The nanocomposites synthesised from Grateloupia lithophila (20 nm) were more effective in seed germination and seedling growth than G. crassa (51 nm) nanocomposites. In this study, one-step synthesised AgNPs from red seaweeds extracts controlled the bacterial pathogens causing disease in rice and promoted the growth of the plant. |
| first_indexed | 2024-03-08T21:48:56Z |
| format | Article |
| id | doaj.art-40ffa7ca870d4a039ed5e09096ec2b27 |
| institution | Directory Open Access Journal |
| issn | 2666-1543 |
| language | English |
| last_indexed | 2024-03-08T21:48:56Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj.art-40ffa7ca870d4a039ed5e09096ec2b272023-12-20T07:37:20ZengElsevierJournal of Agriculture and Food Research2666-15432023-12-0114100769Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applicationsThynraj Antony Roseline0Muthiyal Prabakaran Sudhakar1Kulanthaiyesu Arunkumar2Marine Algae Research Division, Post Graduate and Research Department of Botany, Alagappa Government Arts and Science College (Alagappa University), Karaikudi, 630 003, Tamil Nadu, IndiaMarine Biotechnology Division, National Institute of Ocean Technology (MoES), Chennai, 600100, Tamil Nadu, IndiaMarine Algae Research Division, Post Graduate and Research Department of Botany, Alagappa Government Arts and Science College (Alagappa University), Karaikudi, 630 003, Tamil Nadu, India; Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kerala, India; Corresponding author. Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kasaragod, Kerala, India.In this study, an aqueous extract of Gracilaria crassa and Grateloupia lithophila was used for the green synthesis of silver nanoparticles (AgNPs) to control plant disease and support plant growth. The synthesised AgNPs were characterised using a UV–visible spectrophotometer, FTIR, XRD, SEM, EDAX, TEM and DLS analysis. The TEM image revealed that synthesised nanoparticles with average size of 51 nm and 20 nm were observed in G. crassa and Grateloupia lithophila extract, respectively. The green synthesised AgNPs exhibited a strong antibacterial activity against Xanthomonas axonopodis pv. citri and Xanthomonas oryzae pv. oryzae, and investigated the effect of biogenic AgNPs on seed germination and seedling growth of rice plants (Oryzae sativa L). Both the sizes (51 and 20 nm) of AgNPs treatments (50 μg/ml conc.) resulted in the highest seed germination rate (98 and 99%), longer roots (17.51 ± 0.50 and 17.68 ± 0.10 cm) and shoot lengths (5.68 ± 0.12 and 6.18 ± 0.10 cm), and produced higher amount of fresh (8.98 ± 0.34 and 9.00 ± 0.14 mg) and dry (3.68 ± 0.05 and 3.80 ± 0.12 mg) biomass weights. On the other hand, rice seedlings treated with both sizes of AgNPs, particularly at 100 μg/ml concentration, responded with a lower rate of seed germination, shorter root and shoot length and a lower amount of fresh and dry biomass weight was observed. The nanocomposites synthesised from Grateloupia lithophila (20 nm) were more effective in seed germination and seedling growth than G. crassa (51 nm) nanocomposites. In this study, one-step synthesised AgNPs from red seaweeds extracts controlled the bacterial pathogens causing disease in rice and promoted the growth of the plant.http://www.sciencedirect.com/science/article/pii/S2666154323002764Red seaweedNanocompositesSeed germinationPlant growth promotionDisease controlXanthomonas sp. |
| spellingShingle | Thynraj Antony Roseline Muthiyal Prabakaran Sudhakar Kulanthaiyesu Arunkumar Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications Journal of Agriculture and Food Research Red seaweed Nanocomposites Seed germination Plant growth promotion Disease control Xanthomonas sp. |
| title | Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| title_full | Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| title_fullStr | Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| title_full_unstemmed | Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| title_short | Aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| title_sort | aqueous extraction of red seaweed bioactive compounds and synthesis of silver nanoparticles for agriculture applications |
| topic | Red seaweed Nanocomposites Seed germination Plant growth promotion Disease control Xanthomonas sp. |
| url | http://www.sciencedirect.com/science/article/pii/S2666154323002764 |
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