Detection of gene loci related to drought tolerance in Iranian rice recombinant inbred lines

Objectives: The aim of this study was to determine the linked markers to genes controlling drought tolerance using Iranian rice recombinant inbred lines. Materials and Methods:In this study, 99 Recombinant Inbred Lines of Iranian rice population derived from the cross of Tarom and Khazar were plante...

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Bibliographic Details
Main Authors: Fatemeh Amirkolaei, Hossein Sabouri, Liela Ahangar, Mehdi Zarei, Hossein Hossein Moghaddam
Format: Article
Language:fas
Published: Qom Islamic Azad University 2020-12-01
Series:بیولوژی کاربردی
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Online Access:https://sjoapb.qom.iau.ir/article_681442_8ea1df55dcf61ac11f1fae9d78fe8ba0.pdf
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Summary:Objectives: The aim of this study was to determine the linked markers to genes controlling drought tolerance using Iranian rice recombinant inbred lines. Materials and Methods:In this study, 99 Recombinant Inbred Lines of Iranian rice population derived from the cross of Tarom and Khazar were planted based on randomized complete design in 5 kg pots at the greenhouse of Gonbad Kavous University in 2017. The studied traits were grain weight, Panicle weight, Panicle length, number of Panicle fertile, number of Panicle infertile and number of primary branches. To prepare a genetic map 265 SSR markers, 12 ISSR markers (44 polymorphic alleles), 5 iPBS markers (22 polymorphic alleles) and 2 IRAP markers (8 polymorphic alleles) were used. Results:The markers were used belonged to 12 chromosomes and 1047.4 cM of rice genome were covered. Five QTLs for drought condition and nine QTLs in flooding were located. QTLs related to number of infertile panicle and panicle weight in drought condition mapped between ISSR57-6 and ISSR58-2 markers, on chromosome 1. Also, out of QTLs identified in flooding condition, two QTLs related to the number of primary branches collocated to panicle weights QTLs on chromosomes 5 in IRAP30-1-ISSR2-1 region. qFGW-5, qPB, qFCN-3 and qSP explained a high percentage of phenotypic variation. The detected major effect QTLs in this study can be used in marker-assisted selection breeding programs after validation.
ISSN:2538-3434