Detection of gene loci related to drought tolerance in Iranian rice recombinant inbred lines
Objectives: The aim of this study was to determine the linked markers to genes controlling drought tolerance using Iranian rice recombinant inbred lines. Materials and Methods:In this study, 99 Recombinant Inbred Lines of Iranian rice population derived from the cross of Tarom and Khazar were plante...
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| Format: | Article |
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Qom Islamic Azad University
2020-12-01
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| Series: | بیولوژی کاربردی |
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| Online Access: | https://sjoapb.qom.iau.ir/article_681442_8ea1df55dcf61ac11f1fae9d78fe8ba0.pdf |
| _version_ | 1797778159448358912 |
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| author | Fatemeh Amirkolaei Hossein Sabouri Liela Ahangar Mehdi Zarei Hossein Hossein Moghaddam |
| author_facet | Fatemeh Amirkolaei Hossein Sabouri Liela Ahangar Mehdi Zarei Hossein Hossein Moghaddam |
| author_sort | Fatemeh Amirkolaei |
| collection | DOAJ |
| description | Objectives: The aim of this study was to determine the linked markers to genes controlling drought tolerance using Iranian rice recombinant inbred lines. Materials and Methods:In this study, 99 Recombinant Inbred Lines of Iranian rice population derived from the cross of Tarom and Khazar were planted based on randomized complete design in 5 kg pots at the greenhouse of Gonbad Kavous University in 2017. The studied traits were grain weight, Panicle weight, Panicle length, number of Panicle fertile, number of Panicle infertile and number of primary branches. To prepare a genetic map 265 SSR markers, 12 ISSR markers (44 polymorphic alleles), 5 iPBS markers (22 polymorphic alleles) and 2 IRAP markers (8 polymorphic alleles) were used. Results:The markers were used belonged to 12 chromosomes and 1047.4 cM of rice genome were covered. Five QTLs for drought condition and nine QTLs in flooding were located. QTLs related to number of infertile panicle and panicle weight in drought condition mapped between ISSR57-6 and ISSR58-2 markers, on chromosome 1. Also, out of QTLs identified in flooding condition, two QTLs related to the number of primary branches collocated to panicle weights QTLs on chromosomes 5 in IRAP30-1-ISSR2-1 region. qFGW-5, qPB, qFCN-3 and qSP explained a high percentage of phenotypic variation. The detected major effect QTLs in this study can be used in marker-assisted selection breeding programs after validation. |
| first_indexed | 2024-03-12T23:14:19Z |
| format | Article |
| id | doaj.art-410003d3ce304ca7bf28f9fcf9adfceb |
| institution | Directory Open Access Journal |
| issn | 2538-3434 |
| language | fas |
| last_indexed | 2024-03-12T23:14:19Z |
| publishDate | 2020-12-01 |
| publisher | Qom Islamic Azad University |
| record_format | Article |
| series | بیولوژی کاربردی |
| spelling | doaj.art-410003d3ce304ca7bf28f9fcf9adfceb2023-07-17T14:56:47ZfasQom Islamic Azad Universityبیولوژی کاربردی2538-34342020-12-0110405370681442Detection of gene loci related to drought tolerance in Iranian rice recombinant inbred linesFatemeh Amirkolaei0Hossein Sabouri1Liela Ahangar2Mehdi Zarei3Hossein Hossein Moghaddam4MA., Agricultural Biotechnology, Gonbad Kavous University, Golestan, Iran.Associate Professor, Department of Plant Production, Gonbad Kavous University, Golestan, Iran.Assistants Professor, Department of Plant Production, Gonbad Kavous University, Golestan, Iran.Assistants Professor, Department of Plant Production, Gonbad Kavous University, Golestan, Iran.Assistants Professor, Department of Plant Production, Gonbad Kavous University, Golestan, Iran.Objectives: The aim of this study was to determine the linked markers to genes controlling drought tolerance using Iranian rice recombinant inbred lines. Materials and Methods:In this study, 99 Recombinant Inbred Lines of Iranian rice population derived from the cross of Tarom and Khazar were planted based on randomized complete design in 5 kg pots at the greenhouse of Gonbad Kavous University in 2017. The studied traits were grain weight, Panicle weight, Panicle length, number of Panicle fertile, number of Panicle infertile and number of primary branches. To prepare a genetic map 265 SSR markers, 12 ISSR markers (44 polymorphic alleles), 5 iPBS markers (22 polymorphic alleles) and 2 IRAP markers (8 polymorphic alleles) were used. Results:The markers were used belonged to 12 chromosomes and 1047.4 cM of rice genome were covered. Five QTLs for drought condition and nine QTLs in flooding were located. QTLs related to number of infertile panicle and panicle weight in drought condition mapped between ISSR57-6 and ISSR58-2 markers, on chromosome 1. Also, out of QTLs identified in flooding condition, two QTLs related to the number of primary branches collocated to panicle weights QTLs on chromosomes 5 in IRAP30-1-ISSR2-1 region. qFGW-5, qPB, qFCN-3 and qSP explained a high percentage of phenotypic variation. The detected major effect QTLs in this study can be used in marker-assisted selection breeding programs after validation.https://sjoapb.qom.iau.ir/article_681442_8ea1df55dcf61ac11f1fae9d78fe8ba0.pdfriceqtl markerrecombinant iranian rice linesgene locidrought stress |
| spellingShingle | Fatemeh Amirkolaei Hossein Sabouri Liela Ahangar Mehdi Zarei Hossein Hossein Moghaddam Detection of gene loci related to drought tolerance in Iranian rice recombinant inbred lines بیولوژی کاربردی rice qtl marker recombinant iranian rice lines gene loci drought stress |
| title | Detection of gene loci related to drought tolerance in Iranian rice recombinant
inbred lines |
| title_full | Detection of gene loci related to drought tolerance in Iranian rice recombinant
inbred lines |
| title_fullStr | Detection of gene loci related to drought tolerance in Iranian rice recombinant
inbred lines |
| title_full_unstemmed | Detection of gene loci related to drought tolerance in Iranian rice recombinant
inbred lines |
| title_short | Detection of gene loci related to drought tolerance in Iranian rice recombinant
inbred lines |
| title_sort | detection of gene loci related to drought tolerance in iranian rice recombinant inbred lines |
| topic | rice qtl marker recombinant iranian rice lines gene loci drought stress |
| url | https://sjoapb.qom.iau.ir/article_681442_8ea1df55dcf61ac11f1fae9d78fe8ba0.pdf |
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