Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome
Purpose: To evaluate the changes in cornea in Sjögren’s syndrome (SS) with a novel confocal microscopy device. Methods: Twenty-three right eyes of patients with SS (23 women; mean age, 65.4 ± 11.4 years) and 13 right eyes of 13 age- and sex-matched control subjects (13 women; mean age, 68.8 ± 9.8 ye...
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MDPI AG
2020-07-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/7/497 |
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| author | Yukihiro Matsumoto Osama M. A. Ibrahim Takashi Kojima Murat Dogru Jun Shimazaki Kazuo Tsubota |
| author_facet | Yukihiro Matsumoto Osama M. A. Ibrahim Takashi Kojima Murat Dogru Jun Shimazaki Kazuo Tsubota |
| author_sort | Yukihiro Matsumoto |
| collection | DOAJ |
| description | Purpose: To evaluate the changes in cornea in Sjögren’s syndrome (SS) with a novel confocal microscopy device. Methods: Twenty-three right eyes of patients with SS (23 women; mean age, 65.4 ± 11.4 years) and 13 right eyes of 13 age- and sex-matched control subjects (13 women; mean age, 68.8 ± 9.8 years) were studied. Furthermore, eight right eyes of patients with SS (8 women; mean age, 66.9 ± 9.6 years) were studied to evaluate the corneal microscopic alterations after the treatment with topical 3% diquafosol sodium eye drops. All cases had tear quantity, tear breakup time (BUT), ocular surface staining measurements, and corneal in vivo laser-scanning confocal microscopy examinations. The density and area of corneal epithelial cells (superficial, wing, and basal), density of corneal stromal cells (anterior, intermediate, and posterior), density and area of corneal endothelial cells, density and morphology of corneal sub-basal nerve plexus, density of corneal sub-basal inflammatory cells were also assessed. Results: The tear quantity, stability, and vital staining scores were significantly worse in patients with SS than in control subjects (<i>p</i> < 0.0001). Corneal superficial epithelial cell density was significantly lower in SS compared with control subjects (<i>p</i> < 0.0001). Corneal superficial epithelial cell area was significantly larger in SS compared with control subjects (<i>p</i> = 0.007). Corneal sub-basal nerve fiber density was lower in SS compared with control subjects (<i>p</i> < 0.0001). Morphological abnormality of nerve fibers was observed in SS patients. Corneal sub-basal inflammatory cell density was significantly higher in SS patients compared with control subjects (<i>p</i> < 0.0001). Furthermore, the mean corneal superficial epithelial cell density and area, inflammatory cell density, corneal sub-basal nerve fiber density, and morphological abnormality of nerve fibers, were improved with topical 3% diquafosol sodium treatment in the dry eye patients with SS (<i>p</i> < 0.05). Conclusions: The diagnostic modality using in vivo laser-scanning confocal microscopy was a useful method for the evaluation of the corneal cell density and area, nerve fiber density and morphology, and inflammatory cell density in patients with SS and also a useful tool in the assessment of treatment effect with topical 3% diquafosol sodium in the SS patients. |
| first_indexed | 2024-03-10T18:20:39Z |
| format | Article |
| id | doaj.art-4100981bb6624513babd2e57dab0e75c |
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| language | English |
| last_indexed | 2024-03-10T18:20:39Z |
| publishDate | 2020-07-01 |
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| spelling | doaj.art-4100981bb6624513babd2e57dab0e75c2023-11-20T07:18:50ZengMDPI AGDiagnostics2075-44182020-07-0110749710.3390/diagnostics10070497Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s SyndromeYukihiro Matsumoto0Osama M. A. Ibrahim1Takashi Kojima2Murat Dogru3Jun Shimazaki4Kazuo Tsubota5Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Ophthalmology, Ichikawa General Hospital, Tokyo Dental College, Chiba 272-8513, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, JapanPurpose: To evaluate the changes in cornea in Sjögren’s syndrome (SS) with a novel confocal microscopy device. Methods: Twenty-three right eyes of patients with SS (23 women; mean age, 65.4 ± 11.4 years) and 13 right eyes of 13 age- and sex-matched control subjects (13 women; mean age, 68.8 ± 9.8 years) were studied. Furthermore, eight right eyes of patients with SS (8 women; mean age, 66.9 ± 9.6 years) were studied to evaluate the corneal microscopic alterations after the treatment with topical 3% diquafosol sodium eye drops. All cases had tear quantity, tear breakup time (BUT), ocular surface staining measurements, and corneal in vivo laser-scanning confocal microscopy examinations. The density and area of corneal epithelial cells (superficial, wing, and basal), density of corneal stromal cells (anterior, intermediate, and posterior), density and area of corneal endothelial cells, density and morphology of corneal sub-basal nerve plexus, density of corneal sub-basal inflammatory cells were also assessed. Results: The tear quantity, stability, and vital staining scores were significantly worse in patients with SS than in control subjects (<i>p</i> < 0.0001). Corneal superficial epithelial cell density was significantly lower in SS compared with control subjects (<i>p</i> < 0.0001). Corneal superficial epithelial cell area was significantly larger in SS compared with control subjects (<i>p</i> = 0.007). Corneal sub-basal nerve fiber density was lower in SS compared with control subjects (<i>p</i> < 0.0001). Morphological abnormality of nerve fibers was observed in SS patients. Corneal sub-basal inflammatory cell density was significantly higher in SS patients compared with control subjects (<i>p</i> < 0.0001). Furthermore, the mean corneal superficial epithelial cell density and area, inflammatory cell density, corneal sub-basal nerve fiber density, and morphological abnormality of nerve fibers, were improved with topical 3% diquafosol sodium treatment in the dry eye patients with SS (<i>p</i> < 0.05). Conclusions: The diagnostic modality using in vivo laser-scanning confocal microscopy was a useful method for the evaluation of the corneal cell density and area, nerve fiber density and morphology, and inflammatory cell density in patients with SS and also a useful tool in the assessment of treatment effect with topical 3% diquafosol sodium in the SS patients.https://www.mdpi.com/2075-4418/10/7/497confocal microscopycorneadry eyeSjögren’s syndrome |
| spellingShingle | Yukihiro Matsumoto Osama M. A. Ibrahim Takashi Kojima Murat Dogru Jun Shimazaki Kazuo Tsubota Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome Diagnostics confocal microscopy cornea dry eye Sjögren’s syndrome |
| title | Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome |
| title_full | Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome |
| title_fullStr | Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome |
| title_full_unstemmed | Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome |
| title_short | Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome |
| title_sort | corneal in vivo laser scanning confocal microscopy findings in dry eye patients with sjogren s syndrome |
| topic | confocal microscopy cornea dry eye Sjögren’s syndrome |
| url | https://www.mdpi.com/2075-4418/10/7/497 |
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