Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility
The Corning Epic<sup>®</sup> label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufac...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/8/2043 |
| _version_ | 1797583473969463296 |
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| author | Valentina Bergamini Elisa Resca Alberto Portone Tiziana Petrachi Francesco Ganzerli Stefano Truzzi Giorgio Mari Luigi Rovati Massimo Dominici Elena Veronesi |
| author_facet | Valentina Bergamini Elisa Resca Alberto Portone Tiziana Petrachi Francesco Ganzerli Stefano Truzzi Giorgio Mari Luigi Rovati Massimo Dominici Elena Veronesi |
| author_sort | Valentina Bergamini |
| collection | DOAJ |
| description | The Corning Epic<sup>®</sup> label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufacturing of blood filters, a category of medical devices representing life-saving therapies for the increasing number of patients with kidney failure. The biocompatibility assays were carried out by developing a cell model aimed at mimicking the clinical use of the blood filters and complementing the existing cytotoxicity assay requested by ISO10993-5. Experiments were performed by putting fibroblasts in both direct contact with two types of selected resins, and indirect contact by means of homemade customized well inserts that were precisely designed and developed for this technology. For both types of contact, fibroblasts were cultured in medium and human plasma. ELF tests confirmed the biocompatibility of both resins, highlighting a statistically significant different biological behavior of a polyaromatic resin compared to control and ion-exchanged resin, when materials were in indirect contact and soaking with plasma. Overall, the ELF test is able to mimic clinical scenarios and represents a promising approach to investigate biocompatibility, showing peculiar biological behaviors and suggesting the activation of specific intracellular pathways. |
| first_indexed | 2024-03-10T23:39:23Z |
| format | Article |
| id | doaj.art-4100a4be082f4cb2a0778dd1056c1454 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T23:39:23Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-4100a4be082f4cb2a0778dd1056c14542023-11-19T02:35:51ZengMDPI AGPharmaceutics1999-49232023-07-01158204310.3390/pharmaceutics15082043Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and BiocompatibilityValentina Bergamini0Elisa Resca1Alberto Portone2Tiziana Petrachi3Francesco Ganzerli4Stefano Truzzi5Giorgio Mari6Luigi Rovati7Massimo Dominici8Elena Veronesi9Clinical and Experimental Medicine PhD Program, University of Modena and Reggio Emilia, 41125 Modena, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio, 41037 Mirandola, ItalyThe Corning Epic<sup>®</sup> label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufacturing of blood filters, a category of medical devices representing life-saving therapies for the increasing number of patients with kidney failure. The biocompatibility assays were carried out by developing a cell model aimed at mimicking the clinical use of the blood filters and complementing the existing cytotoxicity assay requested by ISO10993-5. Experiments were performed by putting fibroblasts in both direct contact with two types of selected resins, and indirect contact by means of homemade customized well inserts that were precisely designed and developed for this technology. For both types of contact, fibroblasts were cultured in medium and human plasma. ELF tests confirmed the biocompatibility of both resins, highlighting a statistically significant different biological behavior of a polyaromatic resin compared to control and ion-exchanged resin, when materials were in indirect contact and soaking with plasma. Overall, the ELF test is able to mimic clinical scenarios and represents a promising approach to investigate biocompatibility, showing peculiar biological behaviors and suggesting the activation of specific intracellular pathways.https://www.mdpi.com/1999-4923/15/8/2043label-free technologybiocompatibilitymedical device |
| spellingShingle | Valentina Bergamini Elisa Resca Alberto Portone Tiziana Petrachi Francesco Ganzerli Stefano Truzzi Giorgio Mari Luigi Rovati Massimo Dominici Elena Veronesi Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility Pharmaceutics label-free technology biocompatibility medical device |
| title | Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility |
| title_full | Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility |
| title_fullStr | Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility |
| title_full_unstemmed | Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility |
| title_short | Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility |
| title_sort | label free optical sensing and medical grade resins an advanced approach to investigate cell material interaction and biocompatibility |
| topic | label-free technology biocompatibility medical device |
| url | https://www.mdpi.com/1999-4923/15/8/2043 |
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