Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae
TOR signaling pathway first described in yeast S. сerevisiae is the highly conserved regulator of eukaryotic cell growth, aging and stress resistance. The effect of nitrogen sources, in particular amino acids, on the activity of TOR signaling pathway is well studied, however its relation to carbohyd...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Homza_1_14.pdf |
| _version_ | 1797641810494881792 |
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| author | B. V. Homza R. A. Vasylkovska H. М. Semchyshyn |
| author_facet | B. V. Homza R. A. Vasylkovska H. М. Semchyshyn |
| author_sort | B. V. Homza |
| collection | DOAJ |
| description | TOR signaling pathway first described in yeast S. сerevisiae is the highly conserved regulator of eukaryotic cell growth, aging and stress resistance. The effect of nitrogen sources, in particular amino acids, on the activity of TOR signaling pathway is well studied, however its relation to carbohydrates is poor understood. The aim of the present study is expanding of our understanding of potential role of TOR regulatory complexes in development of carbonyl/oxidative stress that can result from yeast cultivation on glucose and fructose. It has been shown that the level of α-dicarbonyl compounds and protein carbonyl groups increased with time of yeast cultivation and was higher in cells grown on fructose that demonstrated their accelerated aging and carbonyl/oxidative stress development as compared with cells grown on glucose. The strains defective in TOR proteins cultivated in the presence of glucose as well as fructose demonstrated lower markers of the stress and aging than parental strain. Thus these data confirmed the previous conclusion on fructose more potent ability to cause carbonyl/oxidative stress and accelerated aging in S. cerevisiae as compared with glucose. However, defects in TOR regulatory complexes retard aging and development of the stress in yeast independent on the type of carbohydrate in the cultivation medium. |
| first_indexed | 2024-03-11T13:51:04Z |
| format | Article |
| id | doaj.art-41021c942ca64181afee8ecfb3db1f77 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:51:04Z |
| publishDate | 2014-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-41021c942ca64181afee8ecfb3db1f772023-11-02T09:03:57ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032014-02-01861859210.15407/ubj86.01.085Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiaeB. V. Homza0R. A. Vasylkovska1H. М. Semchyshyn2Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineVassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineVassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineTOR signaling pathway first described in yeast S. сerevisiae is the highly conserved regulator of eukaryotic cell growth, aging and stress resistance. The effect of nitrogen sources, in particular amino acids, on the activity of TOR signaling pathway is well studied, however its relation to carbohydrates is poor understood. The aim of the present study is expanding of our understanding of potential role of TOR regulatory complexes in development of carbonyl/oxidative stress that can result from yeast cultivation on glucose and fructose. It has been shown that the level of α-dicarbonyl compounds and protein carbonyl groups increased with time of yeast cultivation and was higher in cells grown on fructose that demonstrated their accelerated aging and carbonyl/oxidative stress development as compared with cells grown on glucose. The strains defective in TOR proteins cultivated in the presence of glucose as well as fructose demonstrated lower markers of the stress and aging than parental strain. Thus these data confirmed the previous conclusion on fructose more potent ability to cause carbonyl/oxidative stress and accelerated aging in S. cerevisiae as compared with glucose. However, defects in TOR regulatory complexes retard aging and development of the stress in yeast independent on the type of carbohydrate in the cultivation medium.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Homza_1_14.pdfagingcarbonyl/oxidative stressfructoseglucoseSaccharomyces cerevisiaeTOR signaling pathway |
| spellingShingle | B. V. Homza R. A. Vasylkovska H. М. Semchyshyn Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae The Ukrainian Biochemical Journal aging carbonyl/oxidative stress fructose glucose Saccharomyces cerevisiae TOR signaling pathway |
| title | Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae |
| title_full | Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae |
| title_fullStr | Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae |
| title_full_unstemmed | Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae |
| title_short | Defects in tor regulatory complexes retard aging and carbonyl/oxidative stress development in yeast Sассharomyces cerevisiae |
| title_sort | defects in tor regulatory complexes retard aging and carbonyl oxidative stress development in yeast sассharomyces cerevisiae |
| topic | aging carbonyl/oxidative stress fructose glucose Saccharomyces cerevisiae TOR signaling pathway |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Homza_1_14.pdf |
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