Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i>
Fragrance is an important characteristic of rose flowers and is largely determined by the terpenes. Rose has a unique NUDX1 (NUDIX HYDROLASES 1)–dependent monoterpene geraniol biosynthesis pathway, but little is known about its transcriptional regulation. In this study, we characterized two China ro...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14684 |
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| author | Jiayao Yu Xiaoyu Liu Yifang Peng Qi Li Yu Han |
| author_facet | Jiayao Yu Xiaoyu Liu Yifang Peng Qi Li Yu Han |
| author_sort | Jiayao Yu |
| collection | DOAJ |
| description | Fragrance is an important characteristic of rose flowers and is largely determined by the terpenes. Rose has a unique NUDX1 (NUDIX HYDROLASES 1)–dependent monoterpene geraniol biosynthesis pathway, but little is known about its transcriptional regulation. In this study, we characterized two China rose (<i>Rosa chinensis</i>) materials from the ‘Old Blush’ variety with contrasting aromas. We profiled the volatile metabolome of both materials, and the results revealed that geraniol was the main component that distinguishes the aroma of these two materials. We performed a comparative transcriptome analysis of the two rose materials, from which we identified the hydrolase RcNUDX1 as a key factor affecting geraniol content, as well as 17 transcription factor genes co-expressed with <i>RcNUDX1</i>. We also determined that the transcription factor RcWRKY70 binds to four W–box motifs in the promoter of <i>RcNUDX1</i>, repressing <i>RcNUDX1</i> expression, based on yeast one-hybrid and transient dual-luciferase assays. These results provide important information concerning the transcriptional regulatory framework underlying the control of geraniol production in rose. |
| first_indexed | 2024-03-09T17:46:19Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:19Z |
| publishDate | 2022-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-41064cc287c54ac6b0eb24901b96abd82023-11-24T11:06:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231468410.3390/ijms232314684Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i>Jiayao Yu0Xiaoyu Liu1Yifang Peng2Qi Li3Yu Han4Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaFragrance is an important characteristic of rose flowers and is largely determined by the terpenes. Rose has a unique NUDX1 (NUDIX HYDROLASES 1)–dependent monoterpene geraniol biosynthesis pathway, but little is known about its transcriptional regulation. In this study, we characterized two China rose (<i>Rosa chinensis</i>) materials from the ‘Old Blush’ variety with contrasting aromas. We profiled the volatile metabolome of both materials, and the results revealed that geraniol was the main component that distinguishes the aroma of these two materials. We performed a comparative transcriptome analysis of the two rose materials, from which we identified the hydrolase RcNUDX1 as a key factor affecting geraniol content, as well as 17 transcription factor genes co-expressed with <i>RcNUDX1</i>. We also determined that the transcription factor RcWRKY70 binds to four W–box motifs in the promoter of <i>RcNUDX1</i>, repressing <i>RcNUDX1</i> expression, based on yeast one-hybrid and transient dual-luciferase assays. These results provide important information concerning the transcriptional regulatory framework underlying the control of geraniol production in rose.https://www.mdpi.com/1422-0067/23/23/14684<i>Rosa chinensis</i>geraniol biosynthesisWGCNA<i>RcNUDX1</i> promoterRcWRKY70 |
| spellingShingle | Jiayao Yu Xiaoyu Liu Yifang Peng Qi Li Yu Han Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> International Journal of Molecular Sciences <i>Rosa chinensis</i> geraniol biosynthesis WGCNA <i>RcNUDX1</i> promoter RcWRKY70 |
| title | Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> |
| title_full | Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> |
| title_fullStr | Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> |
| title_full_unstemmed | Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> |
| title_short | Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis in <i>Rosa chinensis</i> |
| title_sort | identification and characterization of transcription factors involved in geraniol biosynthesis in i rosa chinensis i |
| topic | <i>Rosa chinensis</i> geraniol biosynthesis WGCNA <i>RcNUDX1</i> promoter RcWRKY70 |
| url | https://www.mdpi.com/1422-0067/23/23/14684 |
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