NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity
Abstract Natural killer (NK) cells, key effector cells of the innate immune system, play an important role in the clearance and control of Mycobacterium tuberculosis and HIV infections. Here, we utilized peripheral blood specimens from the Improving Retreatment Success CAPRISA 011 study to character...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38766-7 |
| _version_ | 1827895171006595072 |
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| author | Thando Glory Maseko Santhuri Rambaran Slindile Ngubane Lara Lewis Sinaye Ngcapu Razia Hassan-Moosa Derseree Archary Rubeshan Perumal Nesri Padayatchi Kogieleum Naidoo Aida Sivro |
| author_facet | Thando Glory Maseko Santhuri Rambaran Slindile Ngubane Lara Lewis Sinaye Ngcapu Razia Hassan-Moosa Derseree Archary Rubeshan Perumal Nesri Padayatchi Kogieleum Naidoo Aida Sivro |
| author_sort | Thando Glory Maseko |
| collection | DOAJ |
| description | Abstract Natural killer (NK) cells, key effector cells of the innate immune system, play an important role in the clearance and control of Mycobacterium tuberculosis and HIV infections. Here, we utilized peripheral blood specimens from the Improving Retreatment Success CAPRISA 011 study to characterize NK cell phenotypes during active TB in individuals with or without HIV co-infection. We further assessed the effects of TB treatment on NK cell phenotype, and characterized the effects of NK cell phenotypes during active TB on mycobacterial clearance and TB disease severity measured by the presence of lung cavitation. TB/HIV co-infection led to the expansion of functionally impaired CD56neg NK cell subset. TB treatment completion resulted in restoration of total NK cells, NK cell subset redistribution and downregulation of several NK cell activating and inhibitory receptors. Higher percentage of peripheral CD56bright cells was associated with longer time to culture conversion, while higher expression of NKp46 on CD56dim NK cells was associated with lower odds of lung cavitation in the overall cohort and the TB/HIV co-infected participants. Together these results provide a detailed description of peripheral NK cells in TB and TB/HIV co-infection and yield insights into their role in TB disease pathology. |
| first_indexed | 2024-03-12T22:17:42Z |
| format | Article |
| id | doaj.art-410705ff17ce4b16a4d0c4193c6f43ac |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:17:42Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-410705ff17ce4b16a4d0c4193c6f43ac2023-07-23T11:13:09ZengNature PortfolioScientific Reports2045-23222023-07-0113111010.1038/s41598-023-38766-7NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severityThando Glory Maseko0Santhuri Rambaran1Slindile Ngubane2Lara Lewis3Sinaye Ngcapu4Razia Hassan-Moosa5Derseree Archary6Rubeshan Perumal7Nesri Padayatchi8Kogieleum Naidoo9Aida Sivro10Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Abstract Natural killer (NK) cells, key effector cells of the innate immune system, play an important role in the clearance and control of Mycobacterium tuberculosis and HIV infections. Here, we utilized peripheral blood specimens from the Improving Retreatment Success CAPRISA 011 study to characterize NK cell phenotypes during active TB in individuals with or without HIV co-infection. We further assessed the effects of TB treatment on NK cell phenotype, and characterized the effects of NK cell phenotypes during active TB on mycobacterial clearance and TB disease severity measured by the presence of lung cavitation. TB/HIV co-infection led to the expansion of functionally impaired CD56neg NK cell subset. TB treatment completion resulted in restoration of total NK cells, NK cell subset redistribution and downregulation of several NK cell activating and inhibitory receptors. Higher percentage of peripheral CD56bright cells was associated with longer time to culture conversion, while higher expression of NKp46 on CD56dim NK cells was associated with lower odds of lung cavitation in the overall cohort and the TB/HIV co-infected participants. Together these results provide a detailed description of peripheral NK cells in TB and TB/HIV co-infection and yield insights into their role in TB disease pathology.https://doi.org/10.1038/s41598-023-38766-7 |
| spellingShingle | Thando Glory Maseko Santhuri Rambaran Slindile Ngubane Lara Lewis Sinaye Ngcapu Razia Hassan-Moosa Derseree Archary Rubeshan Perumal Nesri Padayatchi Kogieleum Naidoo Aida Sivro NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity Scientific Reports |
| title | NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity |
| title_full | NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity |
| title_fullStr | NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity |
| title_full_unstemmed | NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity |
| title_short | NK cell phenotypic profile during active TB in people living with HIV-evolution during TB treatment and implications for bacterial clearance and disease severity |
| title_sort | nk cell phenotypic profile during active tb in people living with hiv evolution during tb treatment and implications for bacterial clearance and disease severity |
| url | https://doi.org/10.1038/s41598-023-38766-7 |
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