Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method
This experiment established a relative quantitative detection method for bovine derived components in milk powder based on Real time PCR. The specificity, sensitivity, and stability of cattle-specific primers and probes were tested. By simulating mixed samples of cow’s milk powder and horse’s milk p...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2024-03-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240221 |
| _version_ | 1797238101033091072 |
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| author | CHEN Chen SHI Guo-hua CHEN Bo-xu ZHANG Rui WANG Yu-xin JIA Wen-shen CHEN Jia ZHOU Wei |
| author_facet | CHEN Chen SHI Guo-hua CHEN Bo-xu ZHANG Rui WANG Yu-xin JIA Wen-shen CHEN Jia ZHOU Wei |
| author_sort | CHEN Chen |
| collection | DOAJ |
| description | This experiment established a relative quantitative detection method for bovine derived components in milk powder based on Real time PCR. The specificity, sensitivity, and stability of cattle-specific primers and probes were tested. By simulating mixed samples of cow’s milk powder and horse’s milk powder with different concentrations, linear fitting was performed based on the functional relationship of their △Ct values, and a standard curve was drawn to establish a relative quantitative detection of bovine derived components in milk powder. The results showed that the minimum detection limit of this method was 0.000 01 mg/mL. The recovery rate was 91.11%~119.2%, with an inter group coefficient of variation of ≤0.58% and an intra group coefficient of variation of ≤1.44%. This method is suitable for detecting the adulteration of bovine derived components and content in milk powder in terms of specificity and stability. |
| first_indexed | 2024-04-24T17:30:17Z |
| format | Article |
| id | doaj.art-41072a6444004878bed12a40739c05cb |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-24T17:30:17Z |
| publishDate | 2024-03-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-41072a6444004878bed12a40739c05cb2024-03-28T06:10:10ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612024-03-0132215916410.16210/j.cnki.1007-7561.2024.02.020Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR MethodCHEN Chen0SHI Guo-hua1CHEN Bo-xu2ZHANG Rui3WANG Yu-xin4JIA Wen-shen5 CHEN Jia6ZHOU Wei7Hebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaBeijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCollege of Chemical Technology, Shijiazhuang University, Shijiazhuang, Hebei 050035, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang, Hebei 050227, ChinaThis experiment established a relative quantitative detection method for bovine derived components in milk powder based on Real time PCR. The specificity, sensitivity, and stability of cattle-specific primers and probes were tested. By simulating mixed samples of cow’s milk powder and horse’s milk powder with different concentrations, linear fitting was performed based on the functional relationship of their △Ct values, and a standard curve was drawn to establish a relative quantitative detection of bovine derived components in milk powder. The results showed that the minimum detection limit of this method was 0.000 01 mg/mL. The recovery rate was 91.11%~119.2%, with an inter group coefficient of variation of ≤0.58% and an intra group coefficient of variation of ≤1.44%. This method is suitable for detecting the adulteration of bovine derived components and content in milk powder in terms of specificity and stability.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240221milk powderhorse milk powderreal time pcradulteration detection |
| spellingShingle | CHEN Chen SHI Guo-hua CHEN Bo-xu ZHANG Rui WANG Yu-xin JIA Wen-shen CHEN Jia ZHOU Wei Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method Liang you shipin ke-ji milk powder horse milk powder real time pcr adulteration detection |
| title | Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method |
| title_full | Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method |
| title_fullStr | Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method |
| title_full_unstemmed | Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method |
| title_short | Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method |
| title_sort | quantitative study on the detection of bovine derived components in dairy products based on real time pcr method |
| topic | milk powder horse milk powder real time pcr adulteration detection |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240221 |
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