Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops
Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatograph...
| Main Authors: | , , , , , , , , , , , , , , , |
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PeerJ Inc.
2019-03-01
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| Online Access: | https://peerj.com/articles/6547.pdf |
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| author | Alina Chernova Pavel Mazin Svetlana Goryunova Denis Goryunov Yakov Demurin Lyudmila Gorlova Anna Vanyushkina Waltraud Mair Nikolai Anikanov Ekaterina Yushina Anna Pavlova Elena Martynova Sergei Garkusha Zhanna Mukhina Elena Savenko Philipp Khaitovich |
| author_facet | Alina Chernova Pavel Mazin Svetlana Goryunova Denis Goryunov Yakov Demurin Lyudmila Gorlova Anna Vanyushkina Waltraud Mair Nikolai Anikanov Ekaterina Yushina Anna Pavlova Elena Martynova Sergei Garkusha Zhanna Mukhina Elena Savenko Philipp Khaitovich |
| author_sort | Alina Chernova |
| collection | DOAJ |
| description | Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, while UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher numbers of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain FAs. The longest FA detected using GC-FID was an FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has great potential to be used for the assessment of FA profiles of oil crops. |
| first_indexed | 2024-03-09T07:03:03Z |
| format | Article |
| id | doaj.art-4109f119aa7c4e179c5d62db4eeef862 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:03:03Z |
| publishDate | 2019-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4109f119aa7c4e179c5d62db4eeef8622023-12-03T09:46:51ZengPeerJ Inc.PeerJ2167-83592019-03-017e654710.7717/peerj.6547Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed cropsAlina Chernova0Pavel Mazin1Svetlana Goryunova2Denis Goryunov3Yakov Demurin4Lyudmila Gorlova5Anna Vanyushkina6Waltraud Mair7Nikolai Anikanov8Ekaterina Yushina9Anna Pavlova10Elena Martynova11Sergei Garkusha12Zhanna Mukhina13Elena Savenko14Philipp Khaitovich15Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaPustovoit All-Russia Research Institute of Oil Crops, Krasnodar, RussiaPustovoit All-Russia Research Institute of Oil Crops, Krasnodar, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaAll-Russia Rice Research Institute, Krasnodar, RussiaAll-Russia Rice Research Institute, Krasnodar, RussiaAll-Russia Rice Research Institute, Krasnodar, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaOilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, while UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher numbers of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain FAs. The longest FA detected using GC-FID was an FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has great potential to be used for the assessment of FA profiles of oil crops.https://peerj.com/articles/6547.pdfUltra-performance liquid chromatography-mass spectrometryOil cropsRapeseedFatty acidsBreedingGas chromatography-flame ionization detection |
| spellingShingle | Alina Chernova Pavel Mazin Svetlana Goryunova Denis Goryunov Yakov Demurin Lyudmila Gorlova Anna Vanyushkina Waltraud Mair Nikolai Anikanov Ekaterina Yushina Anna Pavlova Elena Martynova Sergei Garkusha Zhanna Mukhina Elena Savenko Philipp Khaitovich Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops PeerJ Ultra-performance liquid chromatography-mass spectrometry Oil crops Rapeseed Fatty acids Breeding Gas chromatography-flame ionization detection |
| title | Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops |
| title_full | Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops |
| title_fullStr | Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops |
| title_full_unstemmed | Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops |
| title_short | Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops |
| title_sort | ultra performance liquid chromatography mass spectrometry for precise fatty acid profiling of oilseed crops |
| topic | Ultra-performance liquid chromatography-mass spectrometry Oil crops Rapeseed Fatty acids Breeding Gas chromatography-flame ionization detection |
| url | https://peerj.com/articles/6547.pdf |
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