3-D Nucleus Architecture in Oat × Maize Addition Lines
The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chroma...
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4280 |
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| author | Dominika Idziak-Helmcke Tomasz Warzecha Marta Sowa Marzena Warchoł Kinga Dziurka Ilona Czyczyło-Mysza Edyta Skrzypek |
| author_facet | Dominika Idziak-Helmcke Tomasz Warzecha Marta Sowa Marzena Warchoł Kinga Dziurka Ilona Czyczyło-Mysza Edyta Skrzypek |
| author_sort | Dominika Idziak-Helmcke |
| collection | DOAJ |
| description | The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids. |
| first_indexed | 2024-03-10T19:09:01Z |
| format | Article |
| id | doaj.art-410a317a18ab45e7938e15894288034a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:09:01Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-410a317a18ab45e7938e15894288034a2023-11-20T03:58:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112428010.3390/ijms211242803-D Nucleus Architecture in Oat × Maize Addition LinesDominika Idziak-Helmcke0Tomasz Warzecha1Marta Sowa2Marzena Warchoł3Kinga Dziurka4Ilona Czyczyło-Mysza5Edyta Skrzypek6Institute of Biology, Biotechnology, and Environmental Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandDepartment of Plant Breeding, Physiology, and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, PolandInstitute of Biology, Biotechnology, and Environmental Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandDepartment of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, PolandDepartment of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, PolandDepartment of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, PolandDepartment of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, PolandThe nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids.https://www.mdpi.com/1422-0067/21/12/4280addition line<i>Avena sativa</i> L.chromosome territoryGISHhybridnucleus |
| spellingShingle | Dominika Idziak-Helmcke Tomasz Warzecha Marta Sowa Marzena Warchoł Kinga Dziurka Ilona Czyczyło-Mysza Edyta Skrzypek 3-D Nucleus Architecture in Oat × Maize Addition Lines International Journal of Molecular Sciences addition line <i>Avena sativa</i> L. chromosome territory GISH hybrid nucleus |
| title | 3-D Nucleus Architecture in Oat × Maize Addition Lines |
| title_full | 3-D Nucleus Architecture in Oat × Maize Addition Lines |
| title_fullStr | 3-D Nucleus Architecture in Oat × Maize Addition Lines |
| title_full_unstemmed | 3-D Nucleus Architecture in Oat × Maize Addition Lines |
| title_short | 3-D Nucleus Architecture in Oat × Maize Addition Lines |
| title_sort | 3 d nucleus architecture in oat maize addition lines |
| topic | addition line <i>Avena sativa</i> L. chromosome territory GISH hybrid nucleus |
| url | https://www.mdpi.com/1422-0067/21/12/4280 |
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