Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities
The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human ce...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2019.00303/full |
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| author | Juliana Minardi Nascimento Juliana Minardi Nascimento Verônica M. Saia-Cereda Rafaela C. Sartore Rafaela C. Sartore Rodrigo Madeiro da Costa Clarissa S. Schitine Clarissa S. Schitine Hercules Rezende Freitas Hercules Rezende Freitas Michael Murgu Ricardo A. de Melo Reis Stevens K. Rehen Stevens K. Rehen Daniel Martins-de-Souza Daniel Martins-de-Souza Daniel Martins-de-Souza |
| author_facet | Juliana Minardi Nascimento Juliana Minardi Nascimento Verônica M. Saia-Cereda Rafaela C. Sartore Rafaela C. Sartore Rodrigo Madeiro da Costa Clarissa S. Schitine Clarissa S. Schitine Hercules Rezende Freitas Hercules Rezende Freitas Michael Murgu Ricardo A. de Melo Reis Stevens K. Rehen Stevens K. Rehen Daniel Martins-de-Souza Daniel Martins-de-Souza Daniel Martins-de-Souza |
| author_sort | Juliana Minardi Nascimento |
| collection | DOAJ |
| description | The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases. |
| first_indexed | 2024-12-11T03:33:27Z |
| format | Article |
| id | doaj.art-410dbd5994b742e292b6f83126e97324 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-11T03:33:27Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-410dbd5994b742e292b6f83126e973242022-12-22T01:22:19ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2019-11-01710.3389/fcell.2019.00303489908Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic SimilaritiesJuliana Minardi Nascimento0Juliana Minardi Nascimento1Verônica M. Saia-Cereda2Rafaela C. Sartore3Rafaela C. Sartore4Rodrigo Madeiro da Costa5Clarissa S. Schitine6Clarissa S. Schitine7Hercules Rezende Freitas8Hercules Rezende Freitas9Michael Murgu10Ricardo A. de Melo Reis11Stevens K. Rehen12Stevens K. Rehen13Daniel Martins-de-Souza14Daniel Martins-de-Souza15Daniel Martins-de-Souza16Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilD’Or Institute for Research and Education (IDOR), Rio de Janeiro, BrazilLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilD’Or Institute for Research and Education (IDOR), Rio de Janeiro, BrazilNational Institute of Traumatology and Orthopedics, Rio de Janeiro, BrazilD’Or Institute for Research and Education (IDOR), Rio de Janeiro, BrazilInstitute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilSchool of Health Sciences, IBMR – University Center, Rio de Janeiro, BrazilWaters Corporation, Barueri, BrazilInstitute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilD’Or Institute for Research and Education (IDOR), Rio de Janeiro, BrazilInstitute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilInstituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Científico e Tecnológico, São Paulo, BrazilExperimental Medicine Research Cluster (EMRC), University of Campinas, Campinas, BrazilThe limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases.https://www.frontiersin.org/article/10.3389/fcell.2019.00303/fullbrain organoidsneural cellsproteomicsoligodendrocyte progenitorsstem cells |
| spellingShingle | Juliana Minardi Nascimento Juliana Minardi Nascimento Verônica M. Saia-Cereda Rafaela C. Sartore Rafaela C. Sartore Rodrigo Madeiro da Costa Clarissa S. Schitine Clarissa S. Schitine Hercules Rezende Freitas Hercules Rezende Freitas Michael Murgu Ricardo A. de Melo Reis Stevens K. Rehen Stevens K. Rehen Daniel Martins-de-Souza Daniel Martins-de-Souza Daniel Martins-de-Souza Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities Frontiers in Cell and Developmental Biology brain organoids neural cells proteomics oligodendrocyte progenitors stem cells |
| title | Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities |
| title_full | Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities |
| title_fullStr | Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities |
| title_full_unstemmed | Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities |
| title_short | Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities |
| title_sort | human cerebral organoids and fetal brain tissue share proteomic similarities |
| topic | brain organoids neural cells proteomics oligodendrocyte progenitors stem cells |
| url | https://www.frontiersin.org/article/10.3389/fcell.2019.00303/full |
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