| Summary: | <p>Abstract</p> <p>Background</p> <p>To support the positional cloning of the mouse mutation <it>wobbler</it> (<it>wr</it>) the corresponding regions on human Chr2p13-14 and mouse Chr11 were analyzed in detail and compared with respect to gene content, order, and orientation.</p> <p>Results</p> <p>The gene content of the investigated regions was highly conserved between the two species: 20 orthologous genes were identified on our BAC/YAC contig comprising 4.5 Mb between REL/<it>Rel</it> and RAB1A/<it>Rab1a</it>. Exceptions were pseudogenes ELP and PX19 whose mouse counterparts were not located within the analyzed region. Two independently isolated genomic clones indicate an inversion between man and mouse with the inverted segment being identical to the <it>wobbler</it> critical interval. We investigated the <it>wobbler</it> critical region by extensive STS/EST mapping and genomic sequencing. Additionally, the full-length cDNA sequences of four newly mapped genes as well as the previously mapped gene <it>Otx1</it> were established and subjected to mutation analysis. Our data indicate that all genes in the <it>wr</it> critical region have been identified.</p> <p>Conclusion</p> <p>Unexpectedly, neither mutation analysis of cDNAs nor levels of mRNAs indicated which of the candidate genes might be affected by the <it>wr</it> mutation. The possibility arises that there might be hitherto unknown effects of mutations, in addition to structural changes of the mRNA or regulatory abnormalities.</p>
|
|---|