Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14
<p>Abstract</p> <p>Background</p> <p>To support the positional cloning of the mouse mutation <it>wobbler</it> (<it>wr</it>) the corresponding regions on human Chr2p13-14 and mouse Chr11 were analyzed in detail and compared with respect to gene co...
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| Format: | Article |
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BMC
2002-08-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/3/14 |
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| author | Platzer Matthias Ulbrich Michael Thiel Cora Resch Karin Fuchs Sonja Jockusch Harald Schmitt-John Thomas |
| author_facet | Platzer Matthias Ulbrich Michael Thiel Cora Resch Karin Fuchs Sonja Jockusch Harald Schmitt-John Thomas |
| author_sort | Platzer Matthias |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To support the positional cloning of the mouse mutation <it>wobbler</it> (<it>wr</it>) the corresponding regions on human Chr2p13-14 and mouse Chr11 were analyzed in detail and compared with respect to gene content, order, and orientation.</p> <p>Results</p> <p>The gene content of the investigated regions was highly conserved between the two species: 20 orthologous genes were identified on our BAC/YAC contig comprising 4.5 Mb between REL/<it>Rel</it> and RAB1A/<it>Rab1a</it>. Exceptions were pseudogenes ELP and PX19 whose mouse counterparts were not located within the analyzed region. Two independently isolated genomic clones indicate an inversion between man and mouse with the inverted segment being identical to the <it>wobbler</it> critical interval. We investigated the <it>wobbler</it> critical region by extensive STS/EST mapping and genomic sequencing. Additionally, the full-length cDNA sequences of four newly mapped genes as well as the previously mapped gene <it>Otx1</it> were established and subjected to mutation analysis. Our data indicate that all genes in the <it>wr</it> critical region have been identified.</p> <p>Conclusion</p> <p>Unexpectedly, neither mutation analysis of cDNAs nor levels of mRNAs indicated which of the candidate genes might be affected by the <it>wr</it> mutation. The possibility arises that there might be hitherto unknown effects of mutations, in addition to structural changes of the mRNA or regulatory abnormalities.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-13T04:49:34Z |
| publishDate | 2002-08-01 |
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| series | BMC Genetics |
| spelling | doaj.art-410e9d084c33448d94546de69709f7282022-12-22T03:01:43ZengBMCBMC Genetics1471-21562002-08-01311410.1186/1471-2156-3-14Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14Platzer MatthiasUlbrich MichaelThiel CoraResch KarinFuchs SonjaJockusch HaraldSchmitt-John Thomas<p>Abstract</p> <p>Background</p> <p>To support the positional cloning of the mouse mutation <it>wobbler</it> (<it>wr</it>) the corresponding regions on human Chr2p13-14 and mouse Chr11 were analyzed in detail and compared with respect to gene content, order, and orientation.</p> <p>Results</p> <p>The gene content of the investigated regions was highly conserved between the two species: 20 orthologous genes were identified on our BAC/YAC contig comprising 4.5 Mb between REL/<it>Rel</it> and RAB1A/<it>Rab1a</it>. Exceptions were pseudogenes ELP and PX19 whose mouse counterparts were not located within the analyzed region. Two independently isolated genomic clones indicate an inversion between man and mouse with the inverted segment being identical to the <it>wobbler</it> critical interval. We investigated the <it>wobbler</it> critical region by extensive STS/EST mapping and genomic sequencing. Additionally, the full-length cDNA sequences of four newly mapped genes as well as the previously mapped gene <it>Otx1</it> were established and subjected to mutation analysis. Our data indicate that all genes in the <it>wr</it> critical region have been identified.</p> <p>Conclusion</p> <p>Unexpectedly, neither mutation analysis of cDNAs nor levels of mRNAs indicated which of the candidate genes might be affected by the <it>wr</it> mutation. The possibility arises that there might be hitherto unknown effects of mutations, in addition to structural changes of the mRNA or regulatory abnormalities.</p>http://www.biomedcentral.com/1471-2156/3/14 |
| spellingShingle | Platzer Matthias Ulbrich Michael Thiel Cora Resch Karin Fuchs Sonja Jockusch Harald Schmitt-John Thomas Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 BMC Genetics |
| title | Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 |
| title_full | Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 |
| title_fullStr | Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 |
| title_full_unstemmed | Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 |
| title_short | Comparative transcription map of the <it>wobbler</it> critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14 |
| title_sort | comparative transcription map of the it wobbler it critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13 14 |
| url | http://www.biomedcentral.com/1471-2156/3/14 |
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