cagE as a biomarker of the pathogenicity of Helicobacter pylori
Introduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a...
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| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2013-04-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200185 |
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| author | Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva |
| author_facet | Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva |
| author_sort | Ivy Bastos Ramis |
| collection | DOAJ |
| description | Introduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa. |
| first_indexed | 2024-12-12T11:20:28Z |
| format | Article |
| id | doaj.art-41142588d89c4f39ac8dafe1391f27bc |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T11:20:28Z |
| publishDate | 2013-04-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-41142588d89c4f39ac8dafe1391f27bc2022-12-22T00:26:01ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98492013-04-01462185189cagE as a biomarker of the pathogenicity of Helicobacter pyloriIvy Bastos RamisJúlia Silveira ViannaLande Vieira da Silva JuniorAndrea Von GrollPedro Eduardo Almeida da SilvaIntroduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200185Helicobacter pyloriPathogenicity genesEndoscopic diagnosis |
| spellingShingle | Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva cagE as a biomarker of the pathogenicity of Helicobacter pylori Revista da Sociedade Brasileira de Medicina Tropical Helicobacter pylori Pathogenicity genes Endoscopic diagnosis |
| title | cagE as a biomarker of the pathogenicity of Helicobacter pylori |
| title_full | cagE as a biomarker of the pathogenicity of Helicobacter pylori |
| title_fullStr | cagE as a biomarker of the pathogenicity of Helicobacter pylori |
| title_full_unstemmed | cagE as a biomarker of the pathogenicity of Helicobacter pylori |
| title_short | cagE as a biomarker of the pathogenicity of Helicobacter pylori |
| title_sort | cage as a biomarker of the pathogenicity of helicobacter pylori |
| topic | Helicobacter pylori Pathogenicity genes Endoscopic diagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200185 |
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