Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut
Abstract Background Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying p...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04545-9 |
| _version_ | 1797647321654099968 |
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| author | Xiaohui Wu Ziqi Sun Feiyan Qi Hua Liu Mingbo Zhao Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Wenzhao Dong Zheng Zheng Xinyou Zhang |
| author_facet | Xiaohui Wu Ziqi Sun Feiyan Qi Hua Liu Mingbo Zhao Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Wenzhao Dong Zheng Zheng Xinyou Zhang |
| author_sort | Xiaohui Wu |
| collection | DOAJ |
| description | Abstract Background Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying peanut resistance to web blotch is unknown. Results The cytological examination revealed no differences in the conidium germination rate between the web blotch-resistant variety ZH and the web blotch-susceptible variety PI at 12–48 hpi. The appressorium formation rate was significantly higher for PI than for ZH at 24 hpi. The papilla formation rate at 36 hpi and the hypersensitive response rate at 60 and 84 hpi were significantly higher for ZH than for PI. We also compared the transcriptional profiles of web blotch-infected ZH and PI plants at 0, 12, 24, 36, 48, 60, and 84 hpi using an RNA-seq technique. There were more differentially expressed genes (DEGs) in ZH and PI at 12, 36, 60, and 84 hpi than at 24 and 48 hpi. Moreover, there were more DEGs in PI than in ZH at each time-point. The analysis of metabolic pathways indicated that pantothenate and CoA biosynthesis; monobactam biosynthesis; cutin, suberine and wax biosynthesis; and ether lipid metabolism are specific to the active defense of ZH against YY187, whereas porphyrin metabolism as well as taurine and hypotaurine metabolism are pathways specifically involved in the passive defense of ZH against YY187. In the protein-protein interaction (PPI) network, most of the interacting proteins were serine acetyltransferases and cysteine synthases, which are involved in the cysteine synthesis pathway. The qRT-PCR data confirmed the reliability of the transcriptome analysis. Conclusion On the basis of the PPI network for the significantly enriched genes in the pathways which were specifically enriched at different time points in ZH, we hypothesize that serine acetyltransferases and cysteine synthases are crucial for the cysteine-related resistance of peanut to web blotch. The study results provide reference material for future research on the mechanism mediating peanut web blotch resistance. |
| first_indexed | 2024-03-11T15:15:40Z |
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| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-11T15:15:40Z |
| publishDate | 2023-10-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-4114bb5a5b904d50b1d8dec3ea8001562023-10-29T12:19:34ZengBMCBMC Plant Biology1471-22292023-10-0123111910.1186/s12870-023-04545-9Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in PeanutXiaohui Wu0Ziqi Sun1Feiyan Qi2Hua Liu3Mingbo Zhao4Juan Wang5Mengmeng Wang6Ruifang Zhao7Yue Wu8Wenzhao Dong9Zheng Zheng10Xinyou Zhang11The Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesThe Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural SciencesAbstract Background Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying peanut resistance to web blotch is unknown. Results The cytological examination revealed no differences in the conidium germination rate between the web blotch-resistant variety ZH and the web blotch-susceptible variety PI at 12–48 hpi. The appressorium formation rate was significantly higher for PI than for ZH at 24 hpi. The papilla formation rate at 36 hpi and the hypersensitive response rate at 60 and 84 hpi were significantly higher for ZH than for PI. We also compared the transcriptional profiles of web blotch-infected ZH and PI plants at 0, 12, 24, 36, 48, 60, and 84 hpi using an RNA-seq technique. There were more differentially expressed genes (DEGs) in ZH and PI at 12, 36, 60, and 84 hpi than at 24 and 48 hpi. Moreover, there were more DEGs in PI than in ZH at each time-point. The analysis of metabolic pathways indicated that pantothenate and CoA biosynthesis; monobactam biosynthesis; cutin, suberine and wax biosynthesis; and ether lipid metabolism are specific to the active defense of ZH against YY187, whereas porphyrin metabolism as well as taurine and hypotaurine metabolism are pathways specifically involved in the passive defense of ZH against YY187. In the protein-protein interaction (PPI) network, most of the interacting proteins were serine acetyltransferases and cysteine synthases, which are involved in the cysteine synthesis pathway. The qRT-PCR data confirmed the reliability of the transcriptome analysis. Conclusion On the basis of the PPI network for the significantly enriched genes in the pathways which were specifically enriched at different time points in ZH, we hypothesize that serine acetyltransferases and cysteine synthases are crucial for the cysteine-related resistance of peanut to web blotch. The study results provide reference material for future research on the mechanism mediating peanut web blotch resistance.https://doi.org/10.1186/s12870-023-04545-9PeanutWeb blotchCytological examinationRNA-seqCysteine |
| spellingShingle | Xiaohui Wu Ziqi Sun Feiyan Qi Hua Liu Mingbo Zhao Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Wenzhao Dong Zheng Zheng Xinyou Zhang Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut BMC Plant Biology Peanut Web blotch Cytological examination RNA-seq Cysteine |
| title | Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut |
| title_full | Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut |
| title_fullStr | Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut |
| title_full_unstemmed | Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut |
| title_short | Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut |
| title_sort | cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in peanut |
| topic | Peanut Web blotch Cytological examination RNA-seq Cysteine |
| url | https://doi.org/10.1186/s12870-023-04545-9 |
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