Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges
Various biological processes involve the translocation of macromolecules across nanopores; these pores are basically protein channels embedded in membranes. Understanding the mechanism of translocation is crucial to a range of technological applications, including DNA sequencing, single molecule det...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6153 |
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| author | Swarn Lata Singh Keerti Chauhan Atul S. Bharadwaj Vimal Kishore Peter Laux Andreas Luch Ajay Vikram Singh |
| author_facet | Swarn Lata Singh Keerti Chauhan Atul S. Bharadwaj Vimal Kishore Peter Laux Andreas Luch Ajay Vikram Singh |
| author_sort | Swarn Lata Singh |
| collection | DOAJ |
| description | Various biological processes involve the translocation of macromolecules across nanopores; these pores are basically protein channels embedded in membranes. Understanding the mechanism of translocation is crucial to a range of technological applications, including DNA sequencing, single molecule detection, and controlled drug delivery. In this spirit, numerous efforts have been made to develop polymer translocation-based sequencing devices, these efforts include findings and insights from theoretical modeling, simulations, and experimental studies. As much as the past and ongoing studies have added to the knowledge, the practical realization of low-cost, high-throughput sequencing devices, however, has still not been realized. There are challenges, the foremost of which is controlling the speed of translocation at the single monomer level, which remain to be addressed in order to use polymer translocation-based methods for sensing applications. In this article, we review the recent studies aimed at developing control over the dynamics of polymer translocation through nanopores. |
| first_indexed | 2024-03-11T05:35:40Z |
| format | Article |
| id | doaj.art-41157c8f201849c689885abfb7350414 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:35:40Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-41157c8f201849c689885abfb73504142023-11-17T16:47:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247615310.3390/ijms24076153Polymer Translocation and Nanopore Sequencing: A Review of Advances and ChallengesSwarn Lata Singh0Keerti Chauhan1Atul S. Bharadwaj2Vimal Kishore3Peter Laux4Andreas Luch5Ajay Vikram Singh6Department of Physics, Mahila Mahavidyalaya (MMV), Banaras Hindu University, Varanasi 221005, UP, IndiaDepartment of Physics, Banaras Hindu University, Varanasi 221005, UP, IndiaDepartment of Physics, CMP Degree College, University of Allahabad, Prayagraj 211002, UP, IndiaDepartment of Physics, Banaras Hindu University, Varanasi 221005, UP, IndiaDepartment of Chemical and Product Safety, German Federal Institute of Risk Assessment (BfR) Maxdohrnstrasse 8-10, 10589 Berlin, GermanyDepartment of Chemical and Product Safety, German Federal Institute of Risk Assessment (BfR) Maxdohrnstrasse 8-10, 10589 Berlin, GermanyDepartment of Chemical and Product Safety, German Federal Institute of Risk Assessment (BfR) Maxdohrnstrasse 8-10, 10589 Berlin, GermanyVarious biological processes involve the translocation of macromolecules across nanopores; these pores are basically protein channels embedded in membranes. Understanding the mechanism of translocation is crucial to a range of technological applications, including DNA sequencing, single molecule detection, and controlled drug delivery. In this spirit, numerous efforts have been made to develop polymer translocation-based sequencing devices, these efforts include findings and insights from theoretical modeling, simulations, and experimental studies. As much as the past and ongoing studies have added to the knowledge, the practical realization of low-cost, high-throughput sequencing devices, however, has still not been realized. There are challenges, the foremost of which is controlling the speed of translocation at the single monomer level, which remain to be addressed in order to use polymer translocation-based methods for sensing applications. In this article, we review the recent studies aimed at developing control over the dynamics of polymer translocation through nanopores.https://www.mdpi.com/1422-0067/24/7/6153polymer translocationnanopore sequencingtranslocation dynamicsnanopores |
| spellingShingle | Swarn Lata Singh Keerti Chauhan Atul S. Bharadwaj Vimal Kishore Peter Laux Andreas Luch Ajay Vikram Singh Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges International Journal of Molecular Sciences polymer translocation nanopore sequencing translocation dynamics nanopores |
| title | Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges |
| title_full | Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges |
| title_fullStr | Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges |
| title_full_unstemmed | Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges |
| title_short | Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges |
| title_sort | polymer translocation and nanopore sequencing a review of advances and challenges |
| topic | polymer translocation nanopore sequencing translocation dynamics nanopores |
| url | https://www.mdpi.com/1422-0067/24/7/6153 |
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