A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts
Easy and reliable identification of pathogenic species such as yeasts, emerging as problematic microbes originating from the genus <i>Candida</i>, is a task in the management and treatment of infections, especially in hospitals and other healthcare environments. Aptamers are seizing an a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/856 |
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| author | Katharina Kneißle Markus Krämer Ann-Kathrin Kissmann Hu Xing Franziska Müller Valerie Amann Reiner Noschka Kay-Eberhard Gottschalk Anil Bozdogan Jakob Andersson Tanja Weil Barbara Spellerberg Steffen Stenger Frank Rosenau |
| author_facet | Katharina Kneißle Markus Krämer Ann-Kathrin Kissmann Hu Xing Franziska Müller Valerie Amann Reiner Noschka Kay-Eberhard Gottschalk Anil Bozdogan Jakob Andersson Tanja Weil Barbara Spellerberg Steffen Stenger Frank Rosenau |
| author_sort | Katharina Kneißle |
| collection | DOAJ |
| description | Easy and reliable identification of pathogenic species such as yeasts, emerging as problematic microbes originating from the genus <i>Candida</i>, is a task in the management and treatment of infections, especially in hospitals and other healthcare environments. Aptamers are seizing an already indispensable role in different sensing applications as binding entities with almost arbitrarily tunable specificities and optimizable affinities. Here, we describe a polyclonal SELEX library that not only can specifically recognize and fluorescently label <i>Candida</i> cells, but is also capable to differentiate <i>C. albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> cells in flow-cytometry, fluorometric microtiter plate assays and fluorescence microscopy from human cells, exemplified here by human dermal fibroblasts. This offers the opportunity to develop diagnostic tools based on this library. Moreover, these specific and robust affinity molecules could also serve in the future as potent binding entities on biomaterials and as constituents of technical devices and will thus open avenues for the development of cost-effective and easily accessible next generations of electronic biosensors in clinical diagnostics and novel materials for the specific removal of pathogenic cells from human bio-samples. |
| first_indexed | 2024-03-09T13:08:30Z |
| format | Article |
| id | doaj.art-4116f3a396cd4116aeb297163ecfa96a |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T13:08:30Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-4116f3a396cd4116aeb297163ecfa96a2023-11-30T21:45:14ZengMDPI AGJournal of Fungi2309-608X2022-08-018885610.3390/jof8080856A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal FibroblastsKatharina Kneißle0Markus Krämer1Ann-Kathrin Kissmann2Hu Xing3Franziska Müller4Valerie Amann5Reiner Noschka6Kay-Eberhard Gottschalk7Anil Bozdogan8Jakob Andersson9Tanja Weil10Barbara Spellerberg11Steffen Stenger12Frank Rosenau13Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyInstitute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, GermanyInstitute of Experimental Physics, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyCenter for Electrochemical Surface Technology (CEST), Austrian Institute of Technology, 3420 Tulln, AustriaAIT Austrian Institute of Technology, Biosensor Technologies, Giefinggasse 4, 1210 Vienna, AustriaMax Planck Institute for Polymer Research Mainz, Ackermannweg 10, 55128 Mainz, GermanyInstitute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, GermanyInstitute of Medical Microbiology and Hygiene, University Clinic of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, GermanyInstitute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, GermanyEasy and reliable identification of pathogenic species such as yeasts, emerging as problematic microbes originating from the genus <i>Candida</i>, is a task in the management and treatment of infections, especially in hospitals and other healthcare environments. Aptamers are seizing an already indispensable role in different sensing applications as binding entities with almost arbitrarily tunable specificities and optimizable affinities. Here, we describe a polyclonal SELEX library that not only can specifically recognize and fluorescently label <i>Candida</i> cells, but is also capable to differentiate <i>C. albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> cells in flow-cytometry, fluorometric microtiter plate assays and fluorescence microscopy from human cells, exemplified here by human dermal fibroblasts. This offers the opportunity to develop diagnostic tools based on this library. Moreover, these specific and robust affinity molecules could also serve in the future as potent binding entities on biomaterials and as constituents of technical devices and will thus open avenues for the development of cost-effective and easily accessible next generations of electronic biosensors in clinical diagnostics and novel materials for the specific removal of pathogenic cells from human bio-samples.https://www.mdpi.com/2309-608X/8/8/856aptamerbiosensingpathogenic yeastspolyclonal librarySELEX |
| spellingShingle | Katharina Kneißle Markus Krämer Ann-Kathrin Kissmann Hu Xing Franziska Müller Valerie Amann Reiner Noschka Kay-Eberhard Gottschalk Anil Bozdogan Jakob Andersson Tanja Weil Barbara Spellerberg Steffen Stenger Frank Rosenau A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts Journal of Fungi aptamer biosensing pathogenic yeasts polyclonal library SELEX |
| title | A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts |
| title_full | A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts |
| title_fullStr | A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts |
| title_full_unstemmed | A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts |
| title_short | A Polyclonal SELEX Aptamer Library Allows Differentiation of <i>Candida albicans</i>, <i>C. auris</i> and <i>C. parapsilosis</i> Cells from Human Dermal Fibroblasts |
| title_sort | polyclonal selex aptamer library allows differentiation of i candida albicans i i c auris i and i c parapsilosis i cells from human dermal fibroblasts |
| topic | aptamer biosensing pathogenic yeasts polyclonal library SELEX |
| url | https://www.mdpi.com/2309-608X/8/8/856 |
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