Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3
Background: Despite being one of the safest, most effective treatments for severe mood disorders, the therapeutic mechanisms of electroconvulsive therapy remain unknown. Electroconvulsive seizure (ECS) induces rapid, high-level expression of immediate early genes (IEGs) and brain-derived neurotrophi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Brain Stimulation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1935861X23017679 |
| _version_ | 1797798062903525376 |
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| author | K.T. Meyers C.C. Damphousse A.B. Ozols J.M. Campbell J.M. Newbern C. Hu D.F. Marrone A.L. Gallitano |
| author_facet | K.T. Meyers C.C. Damphousse A.B. Ozols J.M. Campbell J.M. Newbern C. Hu D.F. Marrone A.L. Gallitano |
| author_sort | K.T. Meyers |
| collection | DOAJ |
| description | Background: Despite being one of the safest, most effective treatments for severe mood disorders, the therapeutic mechanisms of electroconvulsive therapy remain unknown. Electroconvulsive seizure (ECS) induces rapid, high-level expression of immediate early genes (IEGs) and brain-derived neurotrophic factor (BDNF), in addition to stimulation of neurogenesis and dendritic remodeling of dentate gyrus (DG) neurons. We have previously shown that this upregulation of BDNF fails to occur in the hippocampus of mice lacking the IEG Egr3. Since BDNF influences neurogenesis and dendritic remodeling, we hypothesized that Egr3−/− mice will exhibit deficits in neurogenesis and dendritic remodeling in response to ECS. Objective: To test this hypothesis, we examined dendritic remodeling and cellular proliferation in the DG of Egr3−/− and wild-type mice following repeated ECS. Methods: Mice received 10 daily ECSs. Dendritic morphology was examined in Golgi-Cox-stained tissue and cellular proliferation was analyzed through bromodeoxyuridine (BrdU) immunohistochemistry and confocal imaging. Results: Serial ECS in mice results in dendritic remodeling, increased spine density, and cellular proliferation in the DG. Loss of Egr3 alters the dendritic remodeling induced by serial ECS but does not change the number of dendritic spines or cellular proliferation consequences of ECS. Conclusion: Egr3 influences the dendritic remodeling induced by ECS but is not required for ECS-induced proliferation of hippocampal DG cells. |
| first_indexed | 2024-03-13T03:58:52Z |
| format | Article |
| id | doaj.art-411738107aed4d8c8d9e80f5173ba75c |
| institution | Directory Open Access Journal |
| issn | 1935-861X |
| language | English |
| last_indexed | 2024-03-13T03:58:52Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Stimulation |
| spelling | doaj.art-411738107aed4d8c8d9e80f5173ba75c2023-06-22T05:02:44ZengElsevierBrain Stimulation1935-861X2023-05-01163889900Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3K.T. Meyers0C.C. Damphousse1A.B. Ozols2J.M. Campbell3J.M. Newbern4C. Hu5D.F. Marrone6A.L. Gallitano7Interdisciplinary Graduate Program in Neuroscience, Arizona State University, Tempe, AZ, 85281, USA; Basic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, 85004, USAPsychology, Wilfrid Laurier University, Waterloo, ON, N2L 3C5, CanadaBasic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, 85004, USABasic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, 85004, USASchool of Life Sciences, Arizona State University, Tempe, AZ, 85287, USAEpidemiology and Biostatistics, University of Arizona Mel and Enid Zuckerman College of Public Health – Phoenix, 714 E Van Buren St #119, Phoenix, AZ, 85006, USAPsychology, Wilfrid Laurier University, Waterloo, ON, N2L 3C5, Canada; Corresponding author.Basic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, 85004, USA; Corresponding author.Background: Despite being one of the safest, most effective treatments for severe mood disorders, the therapeutic mechanisms of electroconvulsive therapy remain unknown. Electroconvulsive seizure (ECS) induces rapid, high-level expression of immediate early genes (IEGs) and brain-derived neurotrophic factor (BDNF), in addition to stimulation of neurogenesis and dendritic remodeling of dentate gyrus (DG) neurons. We have previously shown that this upregulation of BDNF fails to occur in the hippocampus of mice lacking the IEG Egr3. Since BDNF influences neurogenesis and dendritic remodeling, we hypothesized that Egr3−/− mice will exhibit deficits in neurogenesis and dendritic remodeling in response to ECS. Objective: To test this hypothesis, we examined dendritic remodeling and cellular proliferation in the DG of Egr3−/− and wild-type mice following repeated ECS. Methods: Mice received 10 daily ECSs. Dendritic morphology was examined in Golgi-Cox-stained tissue and cellular proliferation was analyzed through bromodeoxyuridine (BrdU) immunohistochemistry and confocal imaging. Results: Serial ECS in mice results in dendritic remodeling, increased spine density, and cellular proliferation in the DG. Loss of Egr3 alters the dendritic remodeling induced by serial ECS but does not change the number of dendritic spines or cellular proliferation consequences of ECS. Conclusion: Egr3 influences the dendritic remodeling induced by ECS but is not required for ECS-induced proliferation of hippocampal DG cells.http://www.sciencedirect.com/science/article/pii/S1935861X23017679Early growth response 3Immediate early geneElectroconvulsive seizureElectroconvulsive therapyRepeated ECSNeurogenesis |
| spellingShingle | K.T. Meyers C.C. Damphousse A.B. Ozols J.M. Campbell J.M. Newbern C. Hu D.F. Marrone A.L. Gallitano Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 Brain Stimulation Early growth response 3 Immediate early gene Electroconvulsive seizure Electroconvulsive therapy Repeated ECS Neurogenesis |
| title | Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 |
| title_full | Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 |
| title_fullStr | Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 |
| title_full_unstemmed | Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 |
| title_short | Serial electroconvulsive Seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus; a role for Egr3 |
| title_sort | serial electroconvulsive seizure alters dendritic complexity and promotes cellular proliferation in the mouse dentate gyrus a role for egr3 |
| topic | Early growth response 3 Immediate early gene Electroconvulsive seizure Electroconvulsive therapy Repeated ECS Neurogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S1935861X23017679 |
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