Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae
<i>Spodoptera ornithogalli</i> (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/12/2520 |
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| author | Gloria Patricia Barrera Laura Fernanda Villamizar Gustavo Adolfo Araque Juliana Andrea Gómez Elsa Judith Guevara Carolina Susana Cerrudo Mariano Nicolás Belaich |
| author_facet | Gloria Patricia Barrera Laura Fernanda Villamizar Gustavo Adolfo Araque Juliana Andrea Gómez Elsa Judith Guevara Carolina Susana Cerrudo Mariano Nicolás Belaich |
| author_sort | Gloria Patricia Barrera |
| collection | DOAJ |
| description | <i>Spodoptera ornithogalli</i> (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting <i>S. ornithogalli</i> were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in <i>Baculoviridae</i>, named as <i>Spodoptera ornithogalli nucleopolyhedrovirus</i> (SporNPV) and <i>Spodoptera ornithogalli granulovirus</i> (SporGV). The bioassays carried out in larvae of <i>S. ornithogalli</i> and <i>S. frugiperda</i> showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype. |
| first_indexed | 2024-03-10T03:53:59Z |
| format | Article |
| id | doaj.art-4117acd6cdbf4b1493afad5658dfa98c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T03:53:59Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4117acd6cdbf4b1493afad5658dfa98c2023-11-23T10:59:05ZengMDPI AGViruses1999-49152021-12-011312252010.3390/v13122520Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> LarvaeGloria Patricia Barrera0Laura Fernanda Villamizar1Gustavo Adolfo Araque2Juliana Andrea Gómez3Elsa Judith Guevara4Carolina Susana Cerrudo5Mariano Nicolás Belaich6Corporación Colombiana de Investigación Agropecuaria-Agrosavia, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera, Bogotá 250047, Cundinamarca, ColombiaMicrobial Solutions, AgResearch Ltd., Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New ZealandCorporación Colombiana de Investigación Agropecuaria-Agrosavia, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera, Bogotá 250047, Cundinamarca, ColombiaCorporación Colombiana de Investigación Agropecuaria-Agrosavia, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera, Bogotá 250047, Cundinamarca, ColombiaCorporación Colombiana de Investigación Agropecuaria-Agrosavia, Centro de Investigación Tibaitatá, Kilómetro 14 vía Mosquera, Bogotá 250047, Cundinamarca, ColombiaLaboratorio de Ingeniería Genética y Biología Celular y Molecular—Área Virosis de Insectos (LIGBCM—AVI), Dto. de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal 1876, Provincia de Buenos Aires, ArgentinaLaboratorio de Ingeniería Genética y Biología Celular y Molecular—Área Virosis de Insectos (LIGBCM—AVI), Dto. de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal 1876, Provincia de Buenos Aires, Argentina<i>Spodoptera ornithogalli</i> (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting <i>S. ornithogalli</i> were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in <i>Baculoviridae</i>, named as <i>Spodoptera ornithogalli nucleopolyhedrovirus</i> (SporNPV) and <i>Spodoptera ornithogalli granulovirus</i> (SporGV). The bioassays carried out in larvae of <i>S. ornithogalli</i> and <i>S. frugiperda</i> showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype.https://www.mdpi.com/1999-4915/13/12/2520<i>Spodoptera ornithogalli</i><i>Spodoptera frugiperda</i>SporNPVSporGVnatural coinfection |
| spellingShingle | Gloria Patricia Barrera Laura Fernanda Villamizar Gustavo Adolfo Araque Juliana Andrea Gómez Elsa Judith Guevara Carolina Susana Cerrudo Mariano Nicolás Belaich Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae Viruses <i>Spodoptera ornithogalli</i> <i>Spodoptera frugiperda</i> SporNPV SporGV natural coinfection |
| title | Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae |
| title_full | Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae |
| title_fullStr | Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae |
| title_full_unstemmed | Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae |
| title_short | Natural Coinfection between Novel Species of Baculoviruses in <i>Spodoptera ornithogalli</i> Larvae |
| title_sort | natural coinfection between novel species of baculoviruses in i spodoptera ornithogalli i larvae |
| topic | <i>Spodoptera ornithogalli</i> <i>Spodoptera frugiperda</i> SporNPV SporGV natural coinfection |
| url | https://www.mdpi.com/1999-4915/13/12/2520 |
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