Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions
We investigated the dietary supplementation of protected organic acids and essential Oils P(OA+EO) in pullets raised under commercial conditions. A total of 208,000 pullets Lohmann at 2-weeks-old were assigned to receive 1 of 2 treatments: T1, control diet used in the farm; T2, control diet and P(OA...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2022.1001189/full |
| _version_ | 1811180882553208832 |
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| author | Marcia de Souza Vieira Mariana Lemos de Moraes Thais Bastos Stefanello Francisco Bertolini Junior Guilherme Moreira de Melo Silva João Marcos Novaes Tavares Carlos Yassuharu Nakamatsu Leticia Cury Rocha Veloso Arantes Elizabeth Santin |
| author_facet | Marcia de Souza Vieira Mariana Lemos de Moraes Thais Bastos Stefanello Francisco Bertolini Junior Guilherme Moreira de Melo Silva João Marcos Novaes Tavares Carlos Yassuharu Nakamatsu Leticia Cury Rocha Veloso Arantes Elizabeth Santin |
| author_sort | Marcia de Souza Vieira |
| collection | DOAJ |
| description | We investigated the dietary supplementation of protected organic acids and essential Oils P(OA+EO) in pullets raised under commercial conditions. A total of 208,000 pullets Lohmann at 2-weeks-old were assigned to receive 1 of 2 treatments: T1, control diet used in the farm; T2, control diet and P(OA+EO) at 300 g/t (fumaric, sorbic, citric and malic acids + thymol, eugenol and vanillin microencapsulated in hydrogenated vegetable fat - Jefo Nutrition Inc. Canada). P(OA+EO) was supplemented from 2 to 18 weeks and the trial lasted 21 weeks. At weeks 6, 12 and 21, 12 pullets/treatment were used for blood sampling and necropsy for ISI – I See Inside. A completely randomized design consisting of 2 treatments, each with 12 replicates of 1 hen/replicate, was used. To evaluate intestinal integrity, birds were inoculated with fluorescein-isothiocyanate labelled dextran (FITC-d) and blood samples were collected after 1.5 h. The macroscopic ISI score of alterations were classified to be presented as: overall health ISI (sum of the scores assigned for intestine, liver, proventriculus, annex glands, locomotor, and respiratory systems) and macro-intestinal ISI (sum of the scores assigned for duodenum, jejunum, ileum, and cecum). The histologic intestinal ISI alterations were evaluated in the ileum. A low ISI index represents better health status. Pullets on P(OA+EO) had lower (P< 0.001) levels of FITC-d recovered in the blood, which is related to reduced leaky gut. They also presented lower overall health ISI score at weeks 6 (P = 0.002) and 12 (P = 0.003), lower macro intestinal ISI score at weeks 6 (P = 0.0001) and 21 (P = 0.004) and, lower histologic intestinal ISI score of alterations at weeks 6 (P = 0.09), 12 (P = 0.0006), and 21 (P< 0.0001), which is associated to better overall health. In addition, at week 21, pullets on P(OA+EO) did not present Eimeria oocysts while the control treatment did (P< 0.0001). In conclusion, the blend of protected organic acids and essential oils evaluated can be used to improve intestinal and overall health status in commercial pullets. |
| first_indexed | 2024-04-11T09:09:09Z |
| format | Article |
| id | doaj.art-411964741bc549d78903e962e1509938 |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-04-11T09:09:09Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Animal Science |
| spelling | doaj.art-411964741bc549d78903e962e15099382022-12-22T04:32:32ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252022-09-01310.3389/fanim.2022.10011891001189Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditionsMarcia de Souza Vieira0Mariana Lemos de Moraes1Thais Bastos Stefanello2Francisco Bertolini Junior3Guilherme Moreira de Melo Silva4João Marcos Novaes Tavares5Carlos Yassuharu Nakamatsu6Leticia Cury Rocha Veloso Arantes7Elizabeth Santin8Jefo Nutrition Inc., St-Hyacinthe, QC, CanadaJefo Nutrition Inc., St-Hyacinthe, QC, CanadaJefo Nutrition Inc., St-Hyacinthe, QC, CanadaSafeeds Animal Nutrition, Toledo, BrazilGrupo Mantiqueira, Primavera do Leste, BrazilGrupo Mantiqueira, Primavera do Leste, BrazilGrupo Mantiqueira, Primavera do Leste, BrazilGrupo Mantiqueira, Primavera do Leste, BrazilJefo Nutrition Inc., St-Hyacinthe, QC, CanadaWe investigated the dietary supplementation of protected organic acids and essential Oils P(OA+EO) in pullets raised under commercial conditions. A total of 208,000 pullets Lohmann at 2-weeks-old were assigned to receive 1 of 2 treatments: T1, control diet used in the farm; T2, control diet and P(OA+EO) at 300 g/t (fumaric, sorbic, citric and malic acids + thymol, eugenol and vanillin microencapsulated in hydrogenated vegetable fat - Jefo Nutrition Inc. Canada). P(OA+EO) was supplemented from 2 to 18 weeks and the trial lasted 21 weeks. At weeks 6, 12 and 21, 12 pullets/treatment were used for blood sampling and necropsy for ISI – I See Inside. A completely randomized design consisting of 2 treatments, each with 12 replicates of 1 hen/replicate, was used. To evaluate intestinal integrity, birds were inoculated with fluorescein-isothiocyanate labelled dextran (FITC-d) and blood samples were collected after 1.5 h. The macroscopic ISI score of alterations were classified to be presented as: overall health ISI (sum of the scores assigned for intestine, liver, proventriculus, annex glands, locomotor, and respiratory systems) and macro-intestinal ISI (sum of the scores assigned for duodenum, jejunum, ileum, and cecum). The histologic intestinal ISI alterations were evaluated in the ileum. A low ISI index represents better health status. Pullets on P(OA+EO) had lower (P< 0.001) levels of FITC-d recovered in the blood, which is related to reduced leaky gut. They also presented lower overall health ISI score at weeks 6 (P = 0.002) and 12 (P = 0.003), lower macro intestinal ISI score at weeks 6 (P = 0.0001) and 21 (P = 0.004) and, lower histologic intestinal ISI score of alterations at weeks 6 (P = 0.09), 12 (P = 0.0006), and 21 (P< 0.0001), which is associated to better overall health. In addition, at week 21, pullets on P(OA+EO) did not present Eimeria oocysts while the control treatment did (P< 0.0001). In conclusion, the blend of protected organic acids and essential oils evaluated can be used to improve intestinal and overall health status in commercial pullets.https://www.frontiersin.org/articles/10.3389/fanim.2022.1001189/fullessential oilintestinal healthintestinal permeabilityorganic acidpullets |
| spellingShingle | Marcia de Souza Vieira Mariana Lemos de Moraes Thais Bastos Stefanello Francisco Bertolini Junior Guilherme Moreira de Melo Silva João Marcos Novaes Tavares Carlos Yassuharu Nakamatsu Leticia Cury Rocha Veloso Arantes Elizabeth Santin Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions Frontiers in Animal Science essential oil intestinal health intestinal permeability organic acid pullets |
| title | Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| title_full | Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| title_fullStr | Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| title_full_unstemmed | Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| title_short | Intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| title_sort | intestinal health improvement with protected organic acids and essential oils for pullets raised under field conditions |
| topic | essential oil intestinal health intestinal permeability organic acid pullets |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2022.1001189/full |
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