SARS-CoV-2 entry into and evolution within a skilled nursing facility
Abstract SARS-CoV-2 belongs to the family Coronaviridae which includes multiple human pathogens that have an outsized impact on aging populations. As a novel human pathogen, SARS-CoV-2 is undergoing continuous adaptation to this new host species and there is evidence of this throughout the scientifi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38544-5 |
| _version_ | 1797774274043314176 |
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| author | Nicole R. Sexton Parker J. Cline Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Ashley J. Janich Kristy L. Pabilonia Nicole Ehrhart Gregory D. Ebel |
| author_facet | Nicole R. Sexton Parker J. Cline Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Ashley J. Janich Kristy L. Pabilonia Nicole Ehrhart Gregory D. Ebel |
| author_sort | Nicole R. Sexton |
| collection | DOAJ |
| description | Abstract SARS-CoV-2 belongs to the family Coronaviridae which includes multiple human pathogens that have an outsized impact on aging populations. As a novel human pathogen, SARS-CoV-2 is undergoing continuous adaptation to this new host species and there is evidence of this throughout the scientific and public literature. However, most investigations of SARS-CoV-2 evolution have focused on large-scale collections of data across diverse populations and/or living environments. Here we investigate SARS-CoV-2 evolution in epidemiologically linked individuals within a single outbreak at a skilled nursing facility beginning with initial introduction of the pathogen. The data demonstrate that SARS-CoV-2 was introduced to the facility multiple times without establishing an interfacility transmission chain, followed by a single introduction that infected many individuals within a week. This large-scale introduction by a single genotype then persisted in the facility. SARS-CoV-2 sequences were investigated at both the consensus and intra-host variation levels. Understanding the variability in SARS-CoV-2 during transmission chains will assist in understanding the spread of this disease and can ultimately inform best practices for mitigation strategies. |
| first_indexed | 2024-03-12T22:18:40Z |
| format | Article |
| id | doaj.art-411cea7b5dd842f19895d11babfb6a62 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:18:40Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-411cea7b5dd842f19895d11babfb6a622023-07-23T11:11:50ZengNature PortfolioScientific Reports2045-23222023-07-0113111210.1038/s41598-023-38544-5SARS-CoV-2 entry into and evolution within a skilled nursing facilityNicole R. Sexton0Parker J. Cline1Emily N. Gallichotte2Emily Fitzmeyer3Michael C. Young4Ashley J. Janich5Kristy L. Pabilonia6Nicole Ehrhart7Gregory D. Ebel8Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityColumbine Health Systems Center for Healthy Aging and Department of Clinical Sciences, Colorado State UniversityDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State UniversityAbstract SARS-CoV-2 belongs to the family Coronaviridae which includes multiple human pathogens that have an outsized impact on aging populations. As a novel human pathogen, SARS-CoV-2 is undergoing continuous adaptation to this new host species and there is evidence of this throughout the scientific and public literature. However, most investigations of SARS-CoV-2 evolution have focused on large-scale collections of data across diverse populations and/or living environments. Here we investigate SARS-CoV-2 evolution in epidemiologically linked individuals within a single outbreak at a skilled nursing facility beginning with initial introduction of the pathogen. The data demonstrate that SARS-CoV-2 was introduced to the facility multiple times without establishing an interfacility transmission chain, followed by a single introduction that infected many individuals within a week. This large-scale introduction by a single genotype then persisted in the facility. SARS-CoV-2 sequences were investigated at both the consensus and intra-host variation levels. Understanding the variability in SARS-CoV-2 during transmission chains will assist in understanding the spread of this disease and can ultimately inform best practices for mitigation strategies.https://doi.org/10.1038/s41598-023-38544-5 |
| spellingShingle | Nicole R. Sexton Parker J. Cline Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Ashley J. Janich Kristy L. Pabilonia Nicole Ehrhart Gregory D. Ebel SARS-CoV-2 entry into and evolution within a skilled nursing facility Scientific Reports |
| title | SARS-CoV-2 entry into and evolution within a skilled nursing facility |
| title_full | SARS-CoV-2 entry into and evolution within a skilled nursing facility |
| title_fullStr | SARS-CoV-2 entry into and evolution within a skilled nursing facility |
| title_full_unstemmed | SARS-CoV-2 entry into and evolution within a skilled nursing facility |
| title_short | SARS-CoV-2 entry into and evolution within a skilled nursing facility |
| title_sort | sars cov 2 entry into and evolution within a skilled nursing facility |
| url | https://doi.org/10.1038/s41598-023-38544-5 |
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