Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes
Aims: Obesity is characterized as a chronic state of low-grade inflammation with progressive immune cell infiltration into adipose tissue. Adipose tissue macrophages play a critical role in the establishment of chronic inflammatory states and metabolic dysfunctions. Inonotus (I.) sanghuang and its e...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00286/full |
| _version_ | 1831705900466831360 |
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| author | Mengdi Zhang Yu Xie Yu Xie Xing Su Kun Liu Yijie Zhang Wuyan Pang Junpeng Wang |
| author_facet | Mengdi Zhang Yu Xie Yu Xie Xing Su Kun Liu Yijie Zhang Wuyan Pang Junpeng Wang |
| author_sort | Mengdi Zhang |
| collection | DOAJ |
| description | Aims: Obesity is characterized as a chronic state of low-grade inflammation with progressive immune cell infiltration into adipose tissue. Adipose tissue macrophages play a critical role in the establishment of chronic inflammatory states and metabolic dysfunctions. Inonotus (I.) sanghuang and its extract polyphenols exhibit anti-carcinogenesis, anti-inflammatory, and anti-oxidant activities. However, the action of I. sanghuang polyphenols in obesity-related inflammation has not been reported. The aim of this study was to explore the anti-inflammatory action of polyphenols from I. sanghuang extract (ISE) in macrophages and the interaction between macrophages and adipocytes.Materials and Methods: RAW264.7 macrophages were stimulated with LPS or conditioned medium of hypertrophied 3T3-L1 adipocytes or cocultured with differentiated adipocytes in the presence of different doses of ISE. The inflammatory cytokines were evaluated by ELISA, the MAPK, NF-κB, and IL-6/STAT3 signals were determined by immunoblotting, and the migrated function of macrophages was determined by migration assay.Results: ISE suppressed the inflammatory mediators including NO, TNF-α, IL-6, and MCP-1 induced by either LPS or conditioned medium derived from 3T3-L1 adipocytes. ISE also decreased the production of these inflammatory mediators in cocultures of 3T3-L1 adipocytes and RAW264.7 macrophages. Furthermore, ISE blocked RAW264.7 macrophages migration toward 3T3-L1 adipocytes in cocultures. Finally, this effect of ISE might be mediated via inhibiting ERK, p38, and STAT3 activation.Conclusions: Our findings indicate the possibility that ISE suppresses the interaction between macrophages and adipocytes, attenuates chronic inflammation in adipose tissue and improves obesity-related insulin resistance and complication, suggesting that ISE might be a valuable medicinal food effective in improving insulin resistance and metabolic syndrome. |
| first_indexed | 2024-12-20T16:28:11Z |
| format | Article |
| id | doaj.art-411d65cd4aab4752a93174a3d4ee41db |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T16:28:11Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-411d65cd4aab4752a93174a3d4ee41db2022-12-21T19:33:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-02-011010.3389/fimmu.2019.00286440474Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and AdipocytesMengdi Zhang0Yu Xie1Yu Xie2Xing Su3Kun Liu4Yijie Zhang5Wuyan Pang6Junpeng Wang7Institute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaInstitute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaSchool of Physical Education, Henan University, Kaifeng, ChinaInstitute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaCollege of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang, Hebei, ChinaInstitute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaInstitute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaInstitute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, ChinaAims: Obesity is characterized as a chronic state of low-grade inflammation with progressive immune cell infiltration into adipose tissue. Adipose tissue macrophages play a critical role in the establishment of chronic inflammatory states and metabolic dysfunctions. Inonotus (I.) sanghuang and its extract polyphenols exhibit anti-carcinogenesis, anti-inflammatory, and anti-oxidant activities. However, the action of I. sanghuang polyphenols in obesity-related inflammation has not been reported. The aim of this study was to explore the anti-inflammatory action of polyphenols from I. sanghuang extract (ISE) in macrophages and the interaction between macrophages and adipocytes.Materials and Methods: RAW264.7 macrophages were stimulated with LPS or conditioned medium of hypertrophied 3T3-L1 adipocytes or cocultured with differentiated adipocytes in the presence of different doses of ISE. The inflammatory cytokines were evaluated by ELISA, the MAPK, NF-κB, and IL-6/STAT3 signals were determined by immunoblotting, and the migrated function of macrophages was determined by migration assay.Results: ISE suppressed the inflammatory mediators including NO, TNF-α, IL-6, and MCP-1 induced by either LPS or conditioned medium derived from 3T3-L1 adipocytes. ISE also decreased the production of these inflammatory mediators in cocultures of 3T3-L1 adipocytes and RAW264.7 macrophages. Furthermore, ISE blocked RAW264.7 macrophages migration toward 3T3-L1 adipocytes in cocultures. Finally, this effect of ISE might be mediated via inhibiting ERK, p38, and STAT3 activation.Conclusions: Our findings indicate the possibility that ISE suppresses the interaction between macrophages and adipocytes, attenuates chronic inflammation in adipose tissue and improves obesity-related insulin resistance and complication, suggesting that ISE might be a valuable medicinal food effective in improving insulin resistance and metabolic syndrome.https://www.frontiersin.org/article/10.3389/fimmu.2019.00286/fullInonotus sanghuangpolyphenolsinflammationobesityNF-κB signalingMAPK signaling |
| spellingShingle | Mengdi Zhang Yu Xie Yu Xie Xing Su Kun Liu Yijie Zhang Wuyan Pang Junpeng Wang Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes Frontiers in Immunology Inonotus sanghuang polyphenols inflammation obesity NF-κB signaling MAPK signaling |
| title | Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes |
| title_full | Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes |
| title_fullStr | Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes |
| title_full_unstemmed | Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes |
| title_short | Inonotus sanghuang Polyphenols Attenuate Inflammatory Response Via Modulating the Crosstalk Between Macrophages and Adipocytes |
| title_sort | inonotus sanghuang polyphenols attenuate inflammatory response via modulating the crosstalk between macrophages and adipocytes |
| topic | Inonotus sanghuang polyphenols inflammation obesity NF-κB signaling MAPK signaling |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00286/full |
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