Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized Electroporation

The introduction of plasmids into <i>Agrobacterium</i> cells is one of the key steps in the <i>Agrobacterium</i>-mediated transformation of plants for gene editing applications. Depending on chromosomal background, some <i>Agrobacterium</i> strains exhibit a very...

Full description

Bibliographic Details
Main Authors: Xiang Liu, Joseph F. Miceli, Sabrina Patton, Melissa Murray, John Evans, Xiaoping Wei, Pohao Wang
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Life
Subjects:
Online Access:https://www.mdpi.com/2075-1729/13/11/2217