Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia
The recently discovered high-level natural background radiation area (HBRA) of Mamuju in Indonesia provides a unique opportunity to study the biological effects of chronic low-dose radiation exposure on a human population. The mean total effective dose in the HBRA was approximately 69.6 mSv y<sup...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/12/2384 |
| _version_ | 1797461680318316544 |
|---|---|
| author | Masaru Yamaguchi Yota Tatara Eka Djatnika Nugraha Yuki Tamakuma Yoshiaki Sato Tomisato Miura Masahiro Hosoda Shinji Yoshinaga Mukh Syaifudin Shinji Tokonami Ikuo Kashiwakura |
| author_facet | Masaru Yamaguchi Yota Tatara Eka Djatnika Nugraha Yuki Tamakuma Yoshiaki Sato Tomisato Miura Masahiro Hosoda Shinji Yoshinaga Mukh Syaifudin Shinji Tokonami Ikuo Kashiwakura |
| author_sort | Masaru Yamaguchi |
| collection | DOAJ |
| description | The recently discovered high-level natural background radiation area (HBRA) of Mamuju in Indonesia provides a unique opportunity to study the biological effects of chronic low-dose radiation exposure on a human population. The mean total effective dose in the HBRA was approximately 69.6 mSv y<sup>−1</sup> (range: 47.1 to 115.2 mSv y<sup>−1</sup>), based on a re-evaluation of the individual radiation exposure dose; therefore, proteomic analyses of serum components and oxidative modification profiling of residents living in the HBRA were reconducted using liquid chromatography-tandem mass spectrometry. The analysis of the oxidative modification sequences of human serum albumin revealed significant moderate correlations between the radiation dose and the modification of 12 sequences, especially the 111th methionine, 162nd tyrosine, 356th tyrosine, and 470th methionine residues. In addition, a dose-dependent variation in 15 proteins of the serum components was detected in the serum of residents exposed to chronic low-dose radiation. These findings suggest that the alterations in the expression of specific proteins and the oxidative modification responses of serum albumin found in exposed humans may be important indicators for considering the effects of chronic low-dose radiation exposure on living organisms, implying their potential utility as biomarkers of radiation dose estimation. |
| first_indexed | 2024-03-09T17:23:51Z |
| format | Article |
| id | doaj.art-41216c93b2ce41f28632abdd63c1e7f3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:23:51Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-41216c93b2ce41f28632abdd63c1e7f32023-11-24T12:57:09ZengMDPI AGAntioxidants2076-39212022-12-011112238410.3390/antiox11122384Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, IndonesiaMasaru Yamaguchi0Yota Tatara1Eka Djatnika Nugraha2Yuki Tamakuma3Yoshiaki Sato4Tomisato Miura5Masahiro Hosoda6Shinji Yoshinaga7Mukh Syaifudin8Shinji Tokonami9Ikuo Kashiwakura10Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanGraduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki 036-8562, JapanThe Research Center for Safety, Metrology, and Nuclear Quality Technology, National Research and Innovation Agency of Indonesia, JI. Lebak Bulus Raya No. 49, Jakarta Selatan 12440, IndonesiaCenter for Radiation Research and Education, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, JapanGraduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanInstitute of Radiation Emergency Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanGraduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanResearch Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku 734-8553, JapanThe Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Building 71, Kawasan Puspiptek, Setu, Tangerang Selatan 15310, IndonesiaInstitute of Radiation Emergency Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanGraduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, JapanThe recently discovered high-level natural background radiation area (HBRA) of Mamuju in Indonesia provides a unique opportunity to study the biological effects of chronic low-dose radiation exposure on a human population. The mean total effective dose in the HBRA was approximately 69.6 mSv y<sup>−1</sup> (range: 47.1 to 115.2 mSv y<sup>−1</sup>), based on a re-evaluation of the individual radiation exposure dose; therefore, proteomic analyses of serum components and oxidative modification profiling of residents living in the HBRA were reconducted using liquid chromatography-tandem mass spectrometry. The analysis of the oxidative modification sequences of human serum albumin revealed significant moderate correlations between the radiation dose and the modification of 12 sequences, especially the 111th methionine, 162nd tyrosine, 356th tyrosine, and 470th methionine residues. In addition, a dose-dependent variation in 15 proteins of the serum components was detected in the serum of residents exposed to chronic low-dose radiation. These findings suggest that the alterations in the expression of specific proteins and the oxidative modification responses of serum albumin found in exposed humans may be important indicators for considering the effects of chronic low-dose radiation exposure on living organisms, implying their potential utility as biomarkers of radiation dose estimation.https://www.mdpi.com/2076-3921/11/12/2384high-level natural background radiation arealiquid chromatography-tandem mass spectrometryoxidative modification profilinghuman serum albuminradiation dose estimation |
| spellingShingle | Masaru Yamaguchi Yota Tatara Eka Djatnika Nugraha Yuki Tamakuma Yoshiaki Sato Tomisato Miura Masahiro Hosoda Shinji Yoshinaga Mukh Syaifudin Shinji Tokonami Ikuo Kashiwakura Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia Antioxidants high-level natural background radiation area liquid chromatography-tandem mass spectrometry oxidative modification profiling human serum albumin radiation dose estimation |
| title | Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia |
| title_full | Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia |
| title_fullStr | Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia |
| title_full_unstemmed | Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia |
| title_short | Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia |
| title_sort | oxidative modification status of human serum albumin caused by chronic low dose radiation exposure in mamuju sulawesi indonesia |
| topic | high-level natural background radiation area liquid chromatography-tandem mass spectrometry oxidative modification profiling human serum albumin radiation dose estimation |
| url | https://www.mdpi.com/2076-3921/11/12/2384 |
| work_keys_str_mv | AT masaruyamaguchi oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT yotatatara oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT ekadjatnikanugraha oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT yukitamakuma oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT yoshiakisato oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT tomisatomiura oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT masahirohosoda oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT shinjiyoshinaga oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT mukhsyaifudin oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT shinjitokonami oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia AT ikuokashiwakura oxidativemodificationstatusofhumanserumalbumincausedbychroniclowdoseradiationexposureinmamujusulawesiindonesia |