Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis
Background: As a major disease affecting dairy cow production worldwide, bovine mastitis is caused by a variety of pathogenic microorganisms that eventually cause mammary gland inflammation. Acremonium terricola culture (ATC) is a new type of affordable feed additive produced by the solid fermentati...
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2020-11-01
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| Series: | Food & Nutrition Research |
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| Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/3649/11607 |
| _version_ | 1818554865208524800 |
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| author | Yang Li Xin Jiang Hongjian Xu Jingyi Lv Guangning Zhang Xiujing Dou Yonggen Zhang Xiaoxiang Li |
| author_facet | Yang Li Xin Jiang Hongjian Xu Jingyi Lv Guangning Zhang Xiujing Dou Yonggen Zhang Xiaoxiang Li |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Background: As a major disease affecting dairy cow production worldwide, bovine mastitis is caused by a variety of pathogenic microorganisms that eventually cause mammary gland inflammation. Acremonium terricola culture (ATC) is a new type of affordable feed additive produced by the solid fermentation of A. terricola isolated from Cordyceps gunnii and exerted its anti-inflammatory effect. Objectives: To evaluate the protective effects of ATC on mastitis and investigate its active mechanism, a lipopolysaccharide (LPS)-induced rat mastitis model was used in two experiments. Design: In Experiment 1, a total of 40 female Sprague–Dawley rats were used to determine the optimal supplementary dose of ATC via gavage trial. In Experiment 2, we examined the effects of an optimal dose of ATC on LPS-induced mastitis in rats. Results: The results of Experiment 1 showed that administration of ATC improved growth performance and antioxidant functions in the serum and the liver, as well as immunoglobulin A, G, and M levels in rat serum, and it decreased the content of alanine aminotransferase, aspartate aminotransferase, triglyceride, cholesterol, low-density lipoprotein, and serum urea nitrogen in rat serum; a dosage of 250–1,250 mg/kg/day was shown to be high enough to be effective. The results of Experiment 2 indicated that ATC can relieve the inflammatory reaction of mammary glands in rats, and the LPS-induced expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase significantly decreased after ATC treatment. Moreover, our results demonstrated that ATC markedly enhanced the activity of antioxidase in this rat mastitis model. The results of Western blot analysis revealed that ATC could suppress the expression of toll-like receptor 4, phosphorylation of extracellular signal-regulated kinase, and activity of c-Jun N-terminal kinase in the LPS-stimulated mastitis model. Conclusion: Taken together, ATC was shown to exert its anti-inflammatory effect by blocking mitogen-activated protein kinase signaling pathways. These results demonstrate that ATC exerts anti-inflammatory and antioxidant effects in mastitis prevention. |
| first_indexed | 2024-12-12T09:45:41Z |
| format | Article |
| id | doaj.art-412364110a5b4e549983db2e1ed5caf2 |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-12-12T09:45:41Z |
| publishDate | 2020-11-01 |
| publisher | Swedish Nutrition Foundation |
| record_format | Article |
| series | Food & Nutrition Research |
| spelling | doaj.art-412364110a5b4e549983db2e1ed5caf22022-12-22T00:28:25ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2020-11-0164011310.29219/fnr.v64.36493649Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitisYang Li0Xin Jiang1Hongjian Xu2Jingyi Lv3Guangning Zhang4Xiujing Dou5Yonggen Zhang6Xiaoxiang Li7College of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaMicrobial Biological Engineering Company Limited, Hefei, ChinaBackground: As a major disease affecting dairy cow production worldwide, bovine mastitis is caused by a variety of pathogenic microorganisms that eventually cause mammary gland inflammation. Acremonium terricola culture (ATC) is a new type of affordable feed additive produced by the solid fermentation of A. terricola isolated from Cordyceps gunnii and exerted its anti-inflammatory effect. Objectives: To evaluate the protective effects of ATC on mastitis and investigate its active mechanism, a lipopolysaccharide (LPS)-induced rat mastitis model was used in two experiments. Design: In Experiment 1, a total of 40 female Sprague–Dawley rats were used to determine the optimal supplementary dose of ATC via gavage trial. In Experiment 2, we examined the effects of an optimal dose of ATC on LPS-induced mastitis in rats. Results: The results of Experiment 1 showed that administration of ATC improved growth performance and antioxidant functions in the serum and the liver, as well as immunoglobulin A, G, and M levels in rat serum, and it decreased the content of alanine aminotransferase, aspartate aminotransferase, triglyceride, cholesterol, low-density lipoprotein, and serum urea nitrogen in rat serum; a dosage of 250–1,250 mg/kg/day was shown to be high enough to be effective. The results of Experiment 2 indicated that ATC can relieve the inflammatory reaction of mammary glands in rats, and the LPS-induced expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase significantly decreased after ATC treatment. Moreover, our results demonstrated that ATC markedly enhanced the activity of antioxidase in this rat mastitis model. The results of Western blot analysis revealed that ATC could suppress the expression of toll-like receptor 4, phosphorylation of extracellular signal-regulated kinase, and activity of c-Jun N-terminal kinase in the LPS-stimulated mastitis model. Conclusion: Taken together, ATC was shown to exert its anti-inflammatory effect by blocking mitogen-activated protein kinase signaling pathways. These results demonstrate that ATC exerts anti-inflammatory and antioxidant effects in mastitis prevention.https://foodandnutritionresearch.net/index.php/fnr/article/view/3649/11607acrermonium terricola culturemastitisanti-inflammationantioxidant |
| spellingShingle | Yang Li Xin Jiang Hongjian Xu Jingyi Lv Guangning Zhang Xiujing Dou Yonggen Zhang Xiaoxiang Li Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis Food & Nutrition Research acrermonium terricola culture mastitis anti-inflammation antioxidant |
| title | Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis |
| title_full | Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis |
| title_fullStr | Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis |
| title_full_unstemmed | Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis |
| title_short | Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis |
| title_sort | acremonium terricola culture plays anti inflammatory and antioxidant roles by modulating mapk signaling pathways in rats with lipopolysaccharide induced mastitis |
| topic | acrermonium terricola culture mastitis anti-inflammation antioxidant |
| url | https://foodandnutritionresearch.net/index.php/fnr/article/view/3649/11607 |
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