Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet
Objective To evaluate the inflammation of perirenal adipose tissue induced by high-fat diet in C57BL/6J mice. Methods Mice were randomly divided into normal diet group (control group, n=6) and high-fat diet group (HFD group, n=6). The mRNA expression of TNF-α, CD11c, IL-1β, IL-10, TGF-β1, CD2...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-09-01
|
| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201436.pdf |
| _version_ | 1797365777375952896 |
|---|---|
| author | YANG Yong, LI Zi-yu, YANG Wei, LIU Yong |
| author_facet | YANG Yong, LI Zi-yu, YANG Wei, LIU Yong |
| author_sort | YANG Yong, LI Zi-yu, YANG Wei, LIU Yong |
| collection | DOAJ |
| description | Objective To evaluate the inflammation of perirenal adipose tissue induced by high-fat diet in C57BL/6J mice. Methods Mice were randomly divided into normal diet group (control group, n=6) and high-fat diet group (HFD group, n=6). The mRNA expression of TNF-α, CD11c, IL-1β, IL-10, TGF-β1, CD206 in perirenal adipocytes were detected by RT-qPCR. The expressions of F4/80, CD68 and LCA in perirenal adipose tissue and renal parenchyma were detected by immunohistochemistry method. Results Compared with the control group, the mRNA expression by TNF-α and IL-1β in perirenal adipose tissue of mice in HFD group was increased(P＜0.05); The expression of F4/80, CD68 and LCA in perirenal fat increased in HFD group(P＜0.05). Conclusions High-fat diet induces inflammation in perirenal adipose tissue of C57BL/6J mice. |
| first_indexed | 2024-03-08T16:53:46Z |
| format | Article |
| id | doaj.art-412599a35bf442bc950e4682631dc608 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:46Z |
| publishDate | 2021-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-412599a35bf442bc950e4682631dc6082024-01-05T03:07:03ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-09-0141913181322Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat dietYANG Yong, LI Zi-yu, YANG Wei, LIU Yong01. Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000;;2. Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, ChinaObjective To evaluate the inflammation of perirenal adipose tissue induced by high-fat diet in C57BL/6J mice. Methods Mice were randomly divided into normal diet group (control group, n=6) and high-fat diet group (HFD group, n=6). The mRNA expression of TNF-α, CD11c, IL-1β, IL-10, TGF-β1, CD206 in perirenal adipocytes were detected by RT-qPCR. The expressions of F4/80, CD68 and LCA in perirenal adipose tissue and renal parenchyma were detected by immunohistochemistry method. Results Compared with the control group, the mRNA expression by TNF-α and IL-1β in perirenal adipose tissue of mice in HFD group was increased(P＜0.05); The expression of F4/80, CD68 and LCA in perirenal fat increased in HFD group(P＜0.05). Conclusions High-fat diet induces inflammation in perirenal adipose tissue of C57BL/6J mice.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201436.pdfadipose tissue|high-fat diet|inflammation|kidney|macrophages |
| spellingShingle | YANG Yong, LI Zi-yu, YANG Wei, LIU Yong Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet Jichu yixue yu linchuang adipose tissue|high-fat diet|inflammation|kidney|macrophages |
| title | Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet |
| title_full | Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet |
| title_fullStr | Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet |
| title_full_unstemmed | Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet |
| title_short | Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet |
| title_sort | evaluation of inflammation of perirenal adipose tissue in c57bl 6j mice with high fat diet |
| topic | adipose tissue|high-fat diet|inflammation|kidney|macrophages |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201436.pdf |
| work_keys_str_mv | AT yangyongliziyuyangweiliuyong evaluationofinflammationofperirenaladiposetissueinc57bl6jmicewithhighfatdiet |