Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest
Introduction:Cinnamomi ramulus (CR) is one of the most widely used traditional Chinese medicine (TCM) with anti-cancer effects. Analyzing transcriptomic responses of different human cell lines to TCM treatment is a promising approach to understand the unbiased mechanism of TCM.Methods: This study tr...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1121799/full |
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| author | Jing Li Jing Li Hsi-Yuan Huang Hsi-Yuan Huang Yang-Chi-Dung Lin Yang-Chi-Dung Lin Huali Zuo Huali Zuo Yun Tang Yun Tang Hsien-Da Huang Hsien-Da Huang |
| author_facet | Jing Li Jing Li Hsi-Yuan Huang Hsi-Yuan Huang Yang-Chi-Dung Lin Yang-Chi-Dung Lin Huali Zuo Huali Zuo Yun Tang Yun Tang Hsien-Da Huang Hsien-Da Huang |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Introduction:Cinnamomi ramulus (CR) is one of the most widely used traditional Chinese medicine (TCM) with anti-cancer effects. Analyzing transcriptomic responses of different human cell lines to TCM treatment is a promising approach to understand the unbiased mechanism of TCM.Methods: This study treated ten cancer cell lines with different CR concentrations, followed by mRNA sequencing. Differential expression (DE) analysis and gene set enrichment analysis (GSEA) were utilized to analyze transcriptomic data. Finally, the in silico screening results were verified by in vitro experiments.Results: Both DE and GSEA analysis suggested the Cell cycle pathway was the most perturbated pathway by CR across these cell lines. By analyzing the clinical significance and prognosis of G2/M related genes (PLK1, CDK1, CCNB1, and CCNB2) in various cancer tissues, we found that they were up-regulated in most cancer types, and their down-regulation showed better overall survival rates in cancer patients. Finally, in vitro experiments validation on A549, Hep G2, and HeLa cells suggested that CR can inhibit cell growth by suppressing the PLK1/CDK1/ Cyclin B axis.Discussion: This is the first study to apply transcriptomic analysis to investigate the cancer cell growth inhibition of CR on various human cancer cell lines. The core effect of CR on ten cancer cell lines is to induce G2/M arrest by inhibiting the PLK1/CDK1/Cyclin B axis. |
| first_indexed | 2024-04-09T23:54:30Z |
| format | Article |
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| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T23:54:30Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-41276ba4d83b496cb01a9598088882822023-03-17T04:21:34ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-03-011410.3389/fphar.2023.11217991121799Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrestJing Li0Jing Li1Hsi-Yuan Huang2Hsi-Yuan Huang3Yang-Chi-Dung Lin4Yang-Chi-Dung Lin5Huali Zuo6Huali Zuo7Yun Tang8Yun Tang9Hsien-Da Huang10Hsien-Da Huang11School of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaSchool of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaSchool of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaSchool of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaSchool of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaSchool of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaWarshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong, ChinaIntroduction:Cinnamomi ramulus (CR) is one of the most widely used traditional Chinese medicine (TCM) with anti-cancer effects. Analyzing transcriptomic responses of different human cell lines to TCM treatment is a promising approach to understand the unbiased mechanism of TCM.Methods: This study treated ten cancer cell lines with different CR concentrations, followed by mRNA sequencing. Differential expression (DE) analysis and gene set enrichment analysis (GSEA) were utilized to analyze transcriptomic data. Finally, the in silico screening results were verified by in vitro experiments.Results: Both DE and GSEA analysis suggested the Cell cycle pathway was the most perturbated pathway by CR across these cell lines. By analyzing the clinical significance and prognosis of G2/M related genes (PLK1, CDK1, CCNB1, and CCNB2) in various cancer tissues, we found that they were up-regulated in most cancer types, and their down-regulation showed better overall survival rates in cancer patients. Finally, in vitro experiments validation on A549, Hep G2, and HeLa cells suggested that CR can inhibit cell growth by suppressing the PLK1/CDK1/ Cyclin B axis.Discussion: This is the first study to apply transcriptomic analysis to investigate the cancer cell growth inhibition of CR on various human cancer cell lines. The core effect of CR on ten cancer cell lines is to induce G2/M arrest by inhibiting the PLK1/CDK1/Cyclin B axis.https://www.frontiersin.org/articles/10.3389/fphar.2023.1121799/fullCinnamomi ramulustranscriptomic analysisdifferential expression analysis (DE)gene set enrichment analysis (GSEA)cell cycle |
| spellingShingle | Jing Li Jing Li Hsi-Yuan Huang Hsi-Yuan Huang Yang-Chi-Dung Lin Yang-Chi-Dung Lin Huali Zuo Huali Zuo Yun Tang Yun Tang Hsien-Da Huang Hsien-Da Huang Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest Frontiers in Pharmacology Cinnamomi ramulus transcriptomic analysis differential expression analysis (DE) gene set enrichment analysis (GSEA) cell cycle |
| title | Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest |
| title_full | Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest |
| title_fullStr | Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest |
| title_full_unstemmed | Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest |
| title_short | Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest |
| title_sort | cinnamomi ramulus inhibits cancer cells growth by inducing g2 m arrest |
| topic | Cinnamomi ramulus transcriptomic analysis differential expression analysis (DE) gene set enrichment analysis (GSEA) cell cycle |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1121799/full |
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