The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line
Pyramiding useful QTLs into an elite variety is a promising strategy to develop tolerant varieties against multiple abiotic stresses. However, some QTLs may not be functionally compatible when they are introgressed into the same variety. Here, we tested the functional compatibility of <i>Pup1&...
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MDPI AG
2021-07-01
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| author | Na-Hyun Shin O New Lee Jae-Hyuk Han Kihwan Song Hee-Jong Koh Soo-Cheul Yoo Joong Hyoun Chin |
| author_facet | Na-Hyun Shin O New Lee Jae-Hyuk Han Kihwan Song Hee-Jong Koh Soo-Cheul Yoo Joong Hyoun Chin |
| author_sort | Na-Hyun Shin |
| collection | DOAJ |
| description | Pyramiding useful QTLs into an elite variety is a promising strategy to develop tolerant varieties against multiple abiotic stresses. However, some QTLs may not be functionally compatible when they are introgressed into the same variety. Here, we tested the functional compatibility of <i>Pup1</i> and <i>Sub1</i>, major QTLs for tolerance to phosphorus (P)-deficiency and submergence conditions, respectively. Phenotypic analysis revealed that IR64-Pup1+Sub1 (IPS) plants harboring both <i>Pup1</i> and <i>Sub1</i> QTLs show significant tolerance to submerged conditions, similarly to IR64-Sub1, while IPS failed to tolerate P deficiency and mild drought conditions; only IR64-Pup1 showed P deficiency tolerance. In submerged conditions, <i>Sub1A</i> and <i>OsPSTOL1</i>, major genes for <i>Sub1</i> and <i>Pup1</i> QTLs, respectively, were expressed at the same levels as in IPS and IR64-Sub1 and in IPS and IR64-Pup1, respectively. On the other hand, in P-non-supplied condition, crown root number, root length, and <i>OsPSTOL1</i> expression level were significantly lower in IPS compared to those of IR64-Pup1. However, there was no significant difference in P content between IPS and IR64-Pup1. These results imply that <i>Pup1</i> does not compromise <i>Sub1</i> function in submerged condition, while <i>Sub1</i> suppresses <i>Pup1</i> function in P-non-supplied condition, possibly by regulating the transcript level of <i>Pup1</i>. In conclusion, <i>Pup1</i> and <i>Sub1</i> are regarded as functionally compatible under submergence condition but not under P-non-supplied condition. Further study is needed to elucidate the functional incompatibility of <i>Pup1</i> and <i>Sub1</i> QTLs in IPS under P-non-supplied condition. |
| first_indexed | 2024-03-10T08:28:11Z |
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| language | English |
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| spelling | doaj.art-412999b49e464317a56ec00ebb45954a2023-11-22T09:17:33ZengMDPI AGPlants2223-77472021-07-01108152310.3390/plants10081523The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding LineNa-Hyun Shin0O New Lee1Jae-Hyuk Han2Kihwan Song3Hee-Jong Koh4Soo-Cheul Yoo5Joong Hyoun Chin6Department of Integrative Biological Sciences and Industry, Sejong University, Seoul 05006, KoreaDepartment of Bioindustry and Bioresource Engineering, Plant Engineering Research Institute, Sejong University, Seoul 05006, KoreaDepartment of Integrative Biological Sciences and Industry, Sejong University, Seoul 05006, KoreaDepartment of Bioindustry and Bioresource Engineering, Plant Engineering Research Institute, Sejong University, Seoul 05006, KoreaDepartment of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Plant Life and Environmental Science, Hankyong National University, Anseong 17579, KoreaDepartment of Integrative Biological Sciences and Industry, Sejong University, Seoul 05006, KoreaPyramiding useful QTLs into an elite variety is a promising strategy to develop tolerant varieties against multiple abiotic stresses. However, some QTLs may not be functionally compatible when they are introgressed into the same variety. Here, we tested the functional compatibility of <i>Pup1</i> and <i>Sub1</i>, major QTLs for tolerance to phosphorus (P)-deficiency and submergence conditions, respectively. Phenotypic analysis revealed that IR64-Pup1+Sub1 (IPS) plants harboring both <i>Pup1</i> and <i>Sub1</i> QTLs show significant tolerance to submerged conditions, similarly to IR64-Sub1, while IPS failed to tolerate P deficiency and mild drought conditions; only IR64-Pup1 showed P deficiency tolerance. In submerged conditions, <i>Sub1A</i> and <i>OsPSTOL1</i>, major genes for <i>Sub1</i> and <i>Pup1</i> QTLs, respectively, were expressed at the same levels as in IPS and IR64-Sub1 and in IPS and IR64-Pup1, respectively. On the other hand, in P-non-supplied condition, crown root number, root length, and <i>OsPSTOL1</i> expression level were significantly lower in IPS compared to those of IR64-Pup1. However, there was no significant difference in P content between IPS and IR64-Pup1. These results imply that <i>Pup1</i> does not compromise <i>Sub1</i> function in submerged condition, while <i>Sub1</i> suppresses <i>Pup1</i> function in P-non-supplied condition, possibly by regulating the transcript level of <i>Pup1</i>. In conclusion, <i>Pup1</i> and <i>Sub1</i> are regarded as functionally compatible under submergence condition but not under P-non-supplied condition. Further study is needed to elucidate the functional incompatibility of <i>Pup1</i> and <i>Sub1</i> QTLs in IPS under P-non-supplied condition.https://www.mdpi.com/2223-7747/10/8/1523rice<i>Pup1</i><i>Sub1</i>P deficiency tolerancesubmergence toleranceQTL |
| spellingShingle | Na-Hyun Shin O New Lee Jae-Hyuk Han Kihwan Song Hee-Jong Koh Soo-Cheul Yoo Joong Hyoun Chin The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line Plants rice <i>Pup1</i> <i>Sub1</i> P deficiency tolerance submergence tolerance QTL |
| title | The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line |
| title_full | The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line |
| title_fullStr | The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line |
| title_full_unstemmed | The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line |
| title_short | The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of <i>Pup1</i> in a <i>Pup1</i>+<i>Sub1</i> Breeding Line |
| title_sort | effect of water level in rice cropping system on phosphorus uptake activity of i pup1 i in a i pup1 i i sub1 i breeding line |
| topic | rice <i>Pup1</i> <i>Sub1</i> P deficiency tolerance submergence tolerance QTL |
| url | https://www.mdpi.com/2223-7747/10/8/1523 |
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