Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles
The discovery of plastic and metal nanoparticles in organisms, foods, and beverages has generated numerous studies on the effects of these particles on the barrier cells and their subsequent absorption into the body. Following ingestion, nanoparticles travel down the gastrointestinal tract (GIT), an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/8/708 |
| _version_ | 1797583130661486592 |
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| author | Zainab Jabor Steven C. Sutton |
| author_facet | Zainab Jabor Steven C. Sutton |
| author_sort | Zainab Jabor |
| collection | DOAJ |
| description | The discovery of plastic and metal nanoparticles in organisms, foods, and beverages has generated numerous studies on the effects of these particles on the barrier cells and their subsequent absorption into the body. Following ingestion, nanoparticles travel down the gastrointestinal tract (GIT), and their physicochemical characteristics change in response to the change in proteins and pH during their digestion. We measured the translocation of digested nanoparticles across a co-culture monolayer of Caco-2 and various combinations (1:9, 5:5, and 9:1) of HT29-MTX-E12. The in vitro model of the intestine was used to determine the translocation of digested 20 nm polymethacrylate (PMA) particles and the accompanying monolayer barrier effects after a 72 h exposure. The in vitro digestion increased the agglomeration and hydrodynamic diameters and decreased the surface charge of the nanoparticles. For NH<sub>2</sub>-functionalized polymethacrylate nanoparticles (PMA-NH<sub>2</sub>), the diameters increased from 57 nm (water) to 3800 nm (media), or 2660 nm (chyme). These nanoparticles compromised the integrity of the monolayer (trans-epithelial electrical resistance, Lucifer yellow translocation) and translocated across all the cell ratio configurations. Digestion can have a large effect on nanoparticle agglomeration and surface charge. Excess mucous was not seen as a barrier to the translocation of PMA-NH<sub>2</sub>. |
| first_indexed | 2024-03-10T23:31:29Z |
| format | Article |
| id | doaj.art-412b842059c045d58243759c0be956f5 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T23:31:29Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-412b842059c045d58243759c0be956f52023-11-19T03:14:56ZengMDPI AGToxics2305-63042023-08-0111870810.3390/toxics11080708Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate NanoparticlesZainab Jabor0Steven C. Sutton1School of Pharmacy, Westbrook College of Health Professions, University of New England, 716 Stevens Ave, Portland, ME 04103, USADepartment of Pharmaceutical and Administrative Sciences, School of Pharmacy, Westbrook College of Health Professions, University of New England, 716 Stevens Ave, Portland, ME 04103, USAThe discovery of plastic and metal nanoparticles in organisms, foods, and beverages has generated numerous studies on the effects of these particles on the barrier cells and their subsequent absorption into the body. Following ingestion, nanoparticles travel down the gastrointestinal tract (GIT), and their physicochemical characteristics change in response to the change in proteins and pH during their digestion. We measured the translocation of digested nanoparticles across a co-culture monolayer of Caco-2 and various combinations (1:9, 5:5, and 9:1) of HT29-MTX-E12. The in vitro model of the intestine was used to determine the translocation of digested 20 nm polymethacrylate (PMA) particles and the accompanying monolayer barrier effects after a 72 h exposure. The in vitro digestion increased the agglomeration and hydrodynamic diameters and decreased the surface charge of the nanoparticles. For NH<sub>2</sub>-functionalized polymethacrylate nanoparticles (PMA-NH<sub>2</sub>), the diameters increased from 57 nm (water) to 3800 nm (media), or 2660 nm (chyme). These nanoparticles compromised the integrity of the monolayer (trans-epithelial electrical resistance, Lucifer yellow translocation) and translocated across all the cell ratio configurations. Digestion can have a large effect on nanoparticle agglomeration and surface charge. Excess mucous was not seen as a barrier to the translocation of PMA-NH<sub>2</sub>.https://www.mdpi.com/2305-6304/11/8/708nanoplasticdigestionagglomerationzeta potentialpolystyrenepolymethacrylate |
| spellingShingle | Zainab Jabor Steven C. Sutton Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles Toxics nanoplastic digestion agglomeration zeta potential polystyrene polymethacrylate |
| title | Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles |
| title_full | Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles |
| title_fullStr | Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles |
| title_full_unstemmed | Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles |
| title_short | Effects of Digestion, Cell Culture Media, and Mucous on the Physical Properties, Cellular Effects, and Translocation of Polystyrene and Polymethacrylate Nanoparticles |
| title_sort | effects of digestion cell culture media and mucous on the physical properties cellular effects and translocation of polystyrene and polymethacrylate nanoparticles |
| topic | nanoplastic digestion agglomeration zeta potential polystyrene polymethacrylate |
| url | https://www.mdpi.com/2305-6304/11/8/708 |
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