Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows
(1) Background: There is lack of published studies validating specific cow-side glucometers such as Centrivet GK (CVGK). (2) Methods: The aims were (1) to measure and compare the blood glucose concentrations in 52 tropic highland grassing cows by using CVGK and the traditional enzymatic/photometric...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/22/3536 |
| _version_ | 1797460385477951488 |
|---|---|
| author | Catalina López Valentina Hincapié Jorge U. Carmona |
| author_facet | Catalina López Valentina Hincapié Jorge U. Carmona |
| author_sort | Catalina López |
| collection | DOAJ |
| description | (1) Background: There is lack of published studies validating specific cow-side glucometers such as Centrivet GK (CVGK). (2) Methods: The aims were (1) to measure and compare the blood glucose concentrations in 52 tropic highland grassing cows by using CVGK and the traditional enzymatic/photometric assay (EPA) in plasma and serum (reference method) and (2) to establish if glucose concentrations obtained via these methods could be affected by several demographic and zootechnical parameters of the dairy herd evaluated. (3) Results: Glucose concentrations were significantly (<i>p</i> = 0.00) affected by the method used for their measurement. The intra-assay coefficient of variation (CV) for glucose concentrations in plasma EPA and for CVGK was 14% for both methods with serum EPA, whereas the inter-assay CV for plasma EPA and CVGK was 8% and 13.7%, respectively, with serum EPA. Pearson correlation coefficient calculations between the reference method in serum and plasma presented a slightly positive significant (<i>p</i> = <0.000) correlation (r = 0.56), whereas there was not a significant (<i>p</i> = 0.413) correlation between serum EPA and CVGK (r = 0.135). The Passing and Bablok regressions were out of the ideal expected values for the slope (β = 1) and the intercept (α = 0) (11), whereas the Bland–Altman plots showed a bias of 5.29 ± 11.73 (mg/dL) for serum and plasma and 11.01 ± 15.74 (mg/dL) for serum and CVGK. The ROC curve showed no sensitivity in detecting normoglycemic cows (area = 53.7 %, e.d = 12.5 %, <i>p</i> = 0.759) for CVGK when compared to plasma EPA (area = 36.1 %, e.d = 14.2 %, <i>p</i> = 0.256). Plasma EPA exhibited a better but not significant effect in detecting hyperglycemic cows (area = 63.9%, e.d = 14.2%, <i>p</i> = 0.256) when compared to HHD (area = 46.3 %, e.d = 12.5 %, <i>p</i> = 0.759). General glucose concentrations, independently of the method used, were significantly (<i>p</i> = <0.001) greater in young cows when compared to adult and old cows. (4) Conclusions: Glucose concentration measurement in plasma by using EPA or in capillary blood via CVGK were not reliable methods when compared with the reference method. |
| first_indexed | 2024-03-09T17:05:18Z |
| format | Article |
| id | doaj.art-412d2e3647f04f218cb72241f196a51a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:05:18Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-412d2e3647f04f218cb72241f196a51a2023-11-24T14:24:54ZengMDPI AGAnimals2076-26152023-11-011322353610.3390/ani13223536Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy CowsCatalina López0Valentina Hincapié1Jorge U. Carmona2Grupo de Investigación Patología Clínica Veterinaria, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales 170004, ColombiaGrupo de Investigación Patología Clínica Veterinaria, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales 170004, ColombiaGrupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales 170004, Colombia(1) Background: There is lack of published studies validating specific cow-side glucometers such as Centrivet GK (CVGK). (2) Methods: The aims were (1) to measure and compare the blood glucose concentrations in 52 tropic highland grassing cows by using CVGK and the traditional enzymatic/photometric assay (EPA) in plasma and serum (reference method) and (2) to establish if glucose concentrations obtained via these methods could be affected by several demographic and zootechnical parameters of the dairy herd evaluated. (3) Results: Glucose concentrations were significantly (<i>p</i> = 0.00) affected by the method used for their measurement. The intra-assay coefficient of variation (CV) for glucose concentrations in plasma EPA and for CVGK was 14% for both methods with serum EPA, whereas the inter-assay CV for plasma EPA and CVGK was 8% and 13.7%, respectively, with serum EPA. Pearson correlation coefficient calculations between the reference method in serum and plasma presented a slightly positive significant (<i>p</i> = <0.000) correlation (r = 0.56), whereas there was not a significant (<i>p</i> = 0.413) correlation between serum EPA and CVGK (r = 0.135). The Passing and Bablok regressions were out of the ideal expected values for the slope (β = 1) and the intercept (α = 0) (11), whereas the Bland–Altman plots showed a bias of 5.29 ± 11.73 (mg/dL) for serum and plasma and 11.01 ± 15.74 (mg/dL) for serum and CVGK. The ROC curve showed no sensitivity in detecting normoglycemic cows (area = 53.7 %, e.d = 12.5 %, <i>p</i> = 0.759) for CVGK when compared to plasma EPA (area = 36.1 %, e.d = 14.2 %, <i>p</i> = 0.256). Plasma EPA exhibited a better but not significant effect in detecting hyperglycemic cows (area = 63.9%, e.d = 14.2%, <i>p</i> = 0.256) when compared to HHD (area = 46.3 %, e.d = 12.5 %, <i>p</i> = 0.759). General glucose concentrations, independently of the method used, were significantly (<i>p</i> = <0.001) greater in young cows when compared to adult and old cows. (4) Conclusions: Glucose concentration measurement in plasma by using EPA or in capillary blood via CVGK were not reliable methods when compared with the reference method.https://www.mdpi.com/2076-2615/13/22/3536bovineglucometer validationenergy metabolism |
| spellingShingle | Catalina López Valentina Hincapié Jorge U. Carmona Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows Animals bovine glucometer validation energy metabolism |
| title | Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows |
| title_full | Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows |
| title_fullStr | Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows |
| title_full_unstemmed | Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows |
| title_short | Comparison of Two Methods for the Measurement of Blood Plasma and Capillary Blood Glucose in Tropical Highland Grassing Dairy Cows |
| title_sort | comparison of two methods for the measurement of blood plasma and capillary blood glucose in tropical highland grassing dairy cows |
| topic | bovine glucometer validation energy metabolism |
| url | https://www.mdpi.com/2076-2615/13/22/3536 |
| work_keys_str_mv | AT catalinalopez comparisonoftwomethodsforthemeasurementofbloodplasmaandcapillarybloodglucoseintropicalhighlandgrassingdairycows AT valentinahincapie comparisonoftwomethodsforthemeasurementofbloodplasmaandcapillarybloodglucoseintropicalhighlandgrassingdairycows AT jorgeucarmona comparisonoftwomethodsforthemeasurementofbloodplasmaandcapillarybloodglucoseintropicalhighlandgrassingdairycows |