Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation
In recent years, multiple types of substrates have been applied for regulating cell orientation. Among them, surface topography patterns with grooves or ridges have been widely utilizing for cell culturing. However, this construction is still complicated, low cost-effective and exhibits some technol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/9/9/386 |
| _version_ | 1811325713962237952 |
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| author | Abdelrahman Elbaz Jie Lu Bingbing Gao Fuyin Zheng Zhongde Mu Yuanjin Zhao Zhongze Gu |
| author_facet | Abdelrahman Elbaz Jie Lu Bingbing Gao Fuyin Zheng Zhongde Mu Yuanjin Zhao Zhongze Gu |
| author_sort | Abdelrahman Elbaz |
| collection | DOAJ |
| description | In recent years, multiple types of substrates have been applied for regulating cell orientation. Among them, surface topography patterns with grooves or ridges have been widely utilizing for cell culturing. However, this construction is still complicated, low cost-effective and exhibits some technological limitations with either “top-down” or “bottom-up” approaches. Here, a simple and green method was developed by utilizing butterfly wings (Morpho menelaus, Papilio ulysses telegonus and Ornithoptera croesus lydius) with natural anisotropic nanostructures to generate cell alignment. A two-step chemical treatment was proposed to achieve more hydrophilic butterfly wings preceding cell culturing. Furthermore, calcein acetoxymethyl ester (Calcein-AM) staining and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay results demonstrated the appropriate viability of NIH-3T3 fibroblast cells on those butterfly wings. Moreover, the cells displayed a high degree of alignment in each specimen of these wings. We anticipate that those originating from natural butterfly wings will pose important applications for tissue engineering. |
| first_indexed | 2024-04-13T14:38:43Z |
| format | Article |
| id | doaj.art-412faa1d52f2447aad8fa5f93e497a39 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-04-13T14:38:43Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-412faa1d52f2447aad8fa5f93e497a392022-12-22T02:42:58ZengMDPI AGPolymers2073-43602017-08-019938610.3390/polym9090386polym9090386Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells OrientationAbdelrahman Elbaz0Jie Lu1Bingbing Gao2Fuyin Zheng3Zhongde Mu4Yuanjin Zhao5Zhongze Gu6State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaIn recent years, multiple types of substrates have been applied for regulating cell orientation. Among them, surface topography patterns with grooves or ridges have been widely utilizing for cell culturing. However, this construction is still complicated, low cost-effective and exhibits some technological limitations with either “top-down” or “bottom-up” approaches. Here, a simple and green method was developed by utilizing butterfly wings (Morpho menelaus, Papilio ulysses telegonus and Ornithoptera croesus lydius) with natural anisotropic nanostructures to generate cell alignment. A two-step chemical treatment was proposed to achieve more hydrophilic butterfly wings preceding cell culturing. Furthermore, calcein acetoxymethyl ester (Calcein-AM) staining and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay results demonstrated the appropriate viability of NIH-3T3 fibroblast cells on those butterfly wings. Moreover, the cells displayed a high degree of alignment in each specimen of these wings. We anticipate that those originating from natural butterfly wings will pose important applications for tissue engineering.https://www.mdpi.com/2073-4360/9/9/386butterfly wingsanisotropic nanostructurechemical treatmentcell alignment |
| spellingShingle | Abdelrahman Elbaz Jie Lu Bingbing Gao Fuyin Zheng Zhongde Mu Yuanjin Zhao Zhongze Gu Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation Polymers butterfly wings anisotropic nanostructure chemical treatment cell alignment |
| title | Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation |
| title_full | Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation |
| title_fullStr | Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation |
| title_full_unstemmed | Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation |
| title_short | Chitin-Based Anisotropic Nanostructures of Butterfly Wings for Regulating Cells Orientation |
| title_sort | chitin based anisotropic nanostructures of butterfly wings for regulating cells orientation |
| topic | butterfly wings anisotropic nanostructure chemical treatment cell alignment |
| url | https://www.mdpi.com/2073-4360/9/9/386 |
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