Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI
Magnetite mineralization in human tissue is associated with various pathological processes, especially neurodegenerative disorders. Ferritin’s mineral core is believed to be a precursor of magnetite mineralization. Magnetoferritin (MF) was prepared with different iron loading factors (<i>LFs&l...
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MDPI AG
2021-08-01
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| author | Oliver Strbak Lucia Balejcikova Martina Kmetova Jan Gombos Jozef Kovac Dusan Dobrota Peter Kopcansky |
| author_facet | Oliver Strbak Lucia Balejcikova Martina Kmetova Jan Gombos Jozef Kovac Dusan Dobrota Peter Kopcansky |
| author_sort | Oliver Strbak |
| collection | DOAJ |
| description | Magnetite mineralization in human tissue is associated with various pathological processes, especially neurodegenerative disorders. Ferritin’s mineral core is believed to be a precursor of magnetite mineralization. Magnetoferritin (MF) was prepared with different iron loading factors (<i>LFs</i>) as a model system for pathological ferritin to analyze its MRI relaxivity properties compared to those of native ferritin (NF). The results revealed that MF differs statistically significantly from NF, with the same <i>LF</i>, for all studied relaxation parameters at 7 T: <i>r</i><sub>1</sub>, <i>r</i><sub>2</sub>, <i>r</i><sub>2</sub><i>*</i>, <i>r</i><sub>2</sub><i>/r</i><sub>1</sub>, <i>r</i><sub>2</sub><i>*/r</i><sub>1</sub>. Distinguishability of MF from NF may be useful in non-invasive MRI diagnosis of pathological processes associated with iron accumulation and magnetite mineralization (e.g., neurodegenerative disorders, cancer, and diseases of the heart, lung and liver). In addition, it was found that MF samples possess very strong correlation and MF’s relaxivity is linearly dependent on the <i>LF</i>, and the transverse and longitudinal ratios <i>r</i><sub>2</sub><i>/r</i><sub>1</sub> and <i>r</i><sub>2</sub><i>*/r</i><sub>1</sub> possess complementary information. This is useful in eliminating false-positive hypointensive artefacts and diagnosis of the different stages of pathology. These findings could contribute to the exploitation of MRI techniques in the non-invasive diagnosis of iron-related pathological processes in human tissue. |
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| spelling | doaj.art-4132d575ff60420ab633a514d2bf9db62023-11-22T07:55:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216848710.3390/ijms22168487Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRIOliver Strbak0Lucia Balejcikova1Martina Kmetova2Jan Gombos3Jozef Kovac4Dusan Dobrota5Peter Kopcansky6Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, SlovakiaInstitute of Hydrology, Slovak Academy of Sciences, Dubravska Cesta 9, 841 04 Bratislava, SlovakiaDepartment of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, SlovakiaDepartment of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, SlovakiaInstitute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, SlovakiaDepartment of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, SlovakiaInstitute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, SlovakiaMagnetite mineralization in human tissue is associated with various pathological processes, especially neurodegenerative disorders. Ferritin’s mineral core is believed to be a precursor of magnetite mineralization. Magnetoferritin (MF) was prepared with different iron loading factors (<i>LFs</i>) as a model system for pathological ferritin to analyze its MRI relaxivity properties compared to those of native ferritin (NF). The results revealed that MF differs statistically significantly from NF, with the same <i>LF</i>, for all studied relaxation parameters at 7 T: <i>r</i><sub>1</sub>, <i>r</i><sub>2</sub>, <i>r</i><sub>2</sub><i>*</i>, <i>r</i><sub>2</sub><i>/r</i><sub>1</sub>, <i>r</i><sub>2</sub><i>*/r</i><sub>1</sub>. Distinguishability of MF from NF may be useful in non-invasive MRI diagnosis of pathological processes associated with iron accumulation and magnetite mineralization (e.g., neurodegenerative disorders, cancer, and diseases of the heart, lung and liver). In addition, it was found that MF samples possess very strong correlation and MF’s relaxivity is linearly dependent on the <i>LF</i>, and the transverse and longitudinal ratios <i>r</i><sub>2</sub><i>/r</i><sub>1</sub> and <i>r</i><sub>2</sub><i>*/r</i><sub>1</sub> possess complementary information. This is useful in eliminating false-positive hypointensive artefacts and diagnosis of the different stages of pathology. These findings could contribute to the exploitation of MRI techniques in the non-invasive diagnosis of iron-related pathological processes in human tissue.https://www.mdpi.com/1422-0067/22/16/8487native ferritinmagnetoferritinloading factorMRIrelaxationlongitudinal and transverse relaxivity |
| spellingShingle | Oliver Strbak Lucia Balejcikova Martina Kmetova Jan Gombos Jozef Kovac Dusan Dobrota Peter Kopcansky Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI International Journal of Molecular Sciences native ferritin magnetoferritin loading factor MRI relaxation longitudinal and transverse relaxivity |
| title | Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI |
| title_full | Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI |
| title_fullStr | Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI |
| title_full_unstemmed | Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI |
| title_short | Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI |
| title_sort | longitudinal and transverse relaxivity analysis of native ferritin and magnetoferritin at 7 t mri |
| topic | native ferritin magnetoferritin loading factor MRI relaxation longitudinal and transverse relaxivity |
| url | https://www.mdpi.com/1422-0067/22/16/8487 |
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