ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK
Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Syiah Kuala, Faculty of Mathematics and Natural Science
2019-11-01
|
| Series: | Jurnal Natural |
| Subjects: | |
| Online Access: | http://jurnal.unsyiah.ac.id/natural/article/view/12618 |
| _version_ | 1797230920524103680 |
|---|---|
| author | Ilham Maulana Binawati Ginting Nurdin Nurdin Saiful Fakri |
| author_facet | Ilham Maulana Binawati Ginting Nurdin Nurdin Saiful Fakri |
| author_sort | Ilham Maulana |
| collection | DOAJ |
| description | Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions. |
| first_indexed | 2024-04-24T15:36:09Z |
| format | Article |
| id | doaj.art-4134a49eccb44d2aae9b5da6bea14452 |
| institution | Directory Open Access Journal |
| issn | 1411-8513 2541-4062 |
| language | English |
| last_indexed | 2024-04-24T15:36:09Z |
| publishDate | 2019-11-01 |
| publisher | Universitas Syiah Kuala, Faculty of Mathematics and Natural Science |
| record_format | Article |
| series | Jurnal Natural |
| spelling | doaj.art-4134a49eccb44d2aae9b5da6bea144522024-04-02T01:40:42ZengUniversitas Syiah Kuala, Faculty of Mathematics and Natural ScienceJurnal Natural1411-85132541-40622019-11-01193586310.24815/jn.v19i3.1261810476ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARKIlham Maulana0Binawati Ginting1Nurdin Nurdin2Saiful Fakri3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, IndonesiaAntioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.http://jurnal.unsyiah.ac.id/natural/article/view/12618nutmeg stem bark (myristica fragranshoutt), ethyl acetate extract, dpph, antioksidant |
| spellingShingle | Ilham Maulana Binawati Ginting Nurdin Nurdin Saiful Fakri ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK Jurnal Natural nutmeg stem bark (myristica fragranshoutt), ethyl acetate extract, dpph, antioksidant |
| title | ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK |
| title_full | ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK |
| title_fullStr | ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK |
| title_full_unstemmed | ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK |
| title_short | ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK |
| title_sort | antioxidant activity of ethyl acetate extract of nutmeg myristica fragrans houtt stem bark |
| topic | nutmeg stem bark (myristica fragranshoutt), ethyl acetate extract, dpph, antioksidant |
| url | http://jurnal.unsyiah.ac.id/natural/article/view/12618 |
| work_keys_str_mv | AT ilhammaulana antioxidantactivityofethylacetateextractofnutmegmyristicafragranshouttstembark AT binawatiginting antioxidantactivityofethylacetateextractofnutmegmyristicafragranshouttstembark AT nurdinnurdin antioxidantactivityofethylacetateextractofnutmegmyristicafragranshouttstembark AT saifulfakri antioxidantactivityofethylacetateextractofnutmegmyristicafragranshouttstembark |